FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689149

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689149
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1583933
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC47380.2991288141607No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG32350.20423843685307397No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCCAATTGTTGATCCCTGTATATGAGCTG32300.2039227669352176No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCCAATTGTTGATCCCTGTATATGAGCTGCAGTAATAAT31140.19659922484094972No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT31060.1960941529723795No Hit
CATATACAGGGATCAACAATTGGGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCC28640.18081572894813103No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA26420.166799984595308No Hit
GTCCTGGGCCCACTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCTTGGACAGTCAGTCACCATCTCCTG26260.16578984085816761No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG25680.16212806981103367No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTC25580.1614967299753209No Hit
GATTATTACTGCAGCTCATATACAGGGATCAACAATTGGGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGT25270.1595395764846114No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT23360.14748098562249792No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC23150.14615517196750116No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG22240.14040997946251516No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGCTATAACTATGTCTCCTGGTACCAACAGTA22170.13996804157751622No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG21820.1377583521525216No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC21700.1370007443496663No Hit
ATAATACACAGCCGAGTCCTCGACTCTCAGGTTATTCATGTGCAGAAATA21350.1347910549246717No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21250.13415971508895894No Hit
GGGCACAGGGTCCTGGGCCCACTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCTTGGACAGTCAGTCAC21180.13371777720396003No Hit
CTCTTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTT20880.13182375769682175No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA20700.1306873459925388No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG20410.12885646046897184No Hit
CTGTATATGAGCTGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGGCCGTGTTGC20370.12860392453468675No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCAGCTCATAT20280.1280357186825453No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT19990.12620483315897832No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG19900.12563662730683683No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG19010.12001770276899339No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC18980.11982830081827957No Hit
GTATATGAGCTGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGA18840.11894442504828172No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC18740.11831308521256896No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT18170.1147144481490063No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA18070.11408310831329356No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17990.11357803644472335No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17980.11351490246115208No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA17870.11282042864186805No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC17850.11269416067472551No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGCTATAACTATGTCTCCTGGTACCAACAGTACCCAGGCAGAGC17630.11130521303615747No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT17190.10852731775902137No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA17140.108211647841165No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC17090.10789597792330863No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC17050.10764344198902352No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG17020.1074540400383097No Hit
CAATAATACACAGCCGAGTCCTCGACTCTCAGGTTATTCATGTGCAGAAA16850.10638076231759803No Hit
GTGTATTATTGTGCGACGGATTTCTCCTGGGCCGCGGACGTCTGGGGCCA16470.10398167094188959No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC16390.10347659907331938No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGG16350.10322406313903427No Hit
CCCCTGAGGGCCGCTTAATGACTTGATAAATGATGAGTTTGGGGGCTCTGCCTGGGTACTGTTGGTACCAGGAGA16070.10145631159903859No Hit
CCCAATTGTTGATCCCTGTATATGAGCTGCAGTAATAATCAGCCTCATCC16040.10126690964832477No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT15900.10038303387832692No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10600.043.956561
ATCGCGT503.9999977E-641.1606137
TCGTATG1300.039.74970240
GTGGTAT2750.039.575491
TATACAC4250.036.7935943
GTACGTA500.002471018634.84998369
TTATACA4450.034.3760342
CGAGACT4550.033.62582823
CGTATGC1350.033.23336441
TGGTATC3400.032.994262
TACACAT4700.032.5475235
ACGAGAC4600.032.51915722
GACAGGT2950.032.2808531
AGGGGGG4600.032.08955870
CTTATAC4850.031.5558411
CGAGCCC4800.031.1632115
CCCACGA4800.031.16222819
CGCGTAT450.004694294730.57113639
TCTCCGA4900.030.525311
TCTCGTA1700.030.29990238
Produced by FastQC (version 0.11.5)