FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689150

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689150
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1583933
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG46110.2911107982471481No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC43570.2750747664200443No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG34910.2204007366473203No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCCAATTGTTGATCCCTGTATATGAGCTG32700.20644812627806855No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCCAATTGTTGATCCCTGTATATGAGCTGCAGTAATAAT30250.19098030030310625No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT29440.185866447633833No Hit
CATATACAGGGATCAACAATTGGGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCC27100.17109309547815468No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG25120.15859256673104227No Hit
GTCCTGGGCCCACTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCTTGGACAGTCAGTCACCATCTCCTG24270.15322617812748393No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC23970.15133215862034569No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTC23930.1510796226860606No Hit
GGGCACAGGGTCCTGGGCCCACTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCTTGGACAGTCAGTCAC23920.1510164887024893No Hit
GATTATTACTGCAGCTCATATACAGGGATCAACAATTGGGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGT23910.15095335471891805No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA23820.15038514886677656No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT23660.14937500512963617No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG22980.14508189424678947No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG22810.1440086165260778No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC21090.13314957135181854No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGCTATAACTATGTCTCCTGGTACCAACAGTA20930.13213942761467815No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG20690.13062421200896757No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA20240.1277831827482602No Hit
CTCTTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTT20100.12689930697826235No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG19430.12266933007898692No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG18930.11951263090042319No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCAGCTCATAT18900.11932322894970937No Hit
CTGTATATGAGCTGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGGCCGTGTTGC18850.11900755903185298No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT18670.11787114732757004No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18580.11730294147542858No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACAC18450.11648219968900199No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGCTATAACTATGTCTCCTGGTACCAACAGTACCCAGGCAGAGC18390.11610339578757435No Hit
ATAATACACAGCCGAGTCCTCGACTCTCAGGTTATTCATGTGCAGAAATA18320.11566145790257543No Hit
GTATATGAGCTGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGA17360.10960059547973305No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT17310.10928492556187668No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA17220.10871671970973519No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC17180.10846418377545008No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA17170.10840104979187883No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC17170.10840104979187883No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC16820.1061913603668842No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC16590.1047392787447449No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG16120.10177198151689497No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA16110.1017088475333237No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC16110.1017088475333237No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC16080.10151944558260988No Hit
CCCCTGAGGGCCGCTTAATGACTTGATAAATGATGAGTTTGGGGGCTCTGCCTGGGTACTGTTGGTACCAGGAGA15950.10069870379618329No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15870.1001936319276131No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11000.042.0008961
CGGGATA451.0952501E-437.7566417
GTATCAA30950.031.3919091
GTGGTAT3800.031.2893681
TGGTATC4000.029.7230192
TATCAAC31150.029.4435792
TTGCCGA853.61588E-629.16213456
ATCAACG31400.028.7755183
AATGGTA5100.028.42020670
TCAACGC32050.028.193714
CAACGCA32800.027.5472935
AACGCAG32850.027.5053656
ACGCAGA34600.026.2123727
CGCAGAG34600.026.1141998
AGAGTAC35650.025.33785211
CTCAGCA13850.025.11648470
CAGAGTA35850.025.10891210
TAACCGT951.1856764E-525.029127
AGGTATC1651.4006218E-1024.704852
CGAGTTC901.664154E-423.84776760
Produced by FastQC (version 0.11.5)