FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689151

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689151
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1554821
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT47680.3066590945195621No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA43360.27887454568725273No Hit
CCCCTGTGCTGGTCATCTATCAAGATACCAAGCGGCCCTCAGGGATCCCT40510.2605444613881598No Hit
CCTATGAGCTGACTCAGCCACCCTCAGTGTCCGTGTCCCCAGGACAGACA39640.25494896197054195No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG37420.24067079104282746No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC37030.2381624637176884No Hit
GTAATACACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGAAACA36890.23726203852404873No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC35770.23005863697493154No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC33990.2186103737986559No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC33770.21719541992293645No Hit
GCACAGGGGACTGGCCTGGCTTCTGCTGATACCAACAAGCATATTTATCC32260.2074836910486802No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC29300.18844612981172754No Hit
CTATTACTGTCAGGCGTGGGACAGCAGCACTGCTCTTTATGTCTTCGGAC29170.18761002070334784No Hit
GACCTAGGACGGTGACCTTGGTCCCCGGTCCGAAGACATAAAGAGCAGTG28040.18034230306897064No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC28000.18008503872793075No Hit
GACTATTACTGTCAGGCGTGGGACAGCAGCACTGCTCTTTATGTCTTCGG27840.17905598136377113No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCCGGTCCGAAGACATAAAGAGCAGTGCTGCTGTCCCACGC27750.17847713659643136No Hit
ACCTAGGACGGTGACCTTGGTCCCCGGTCCGAAGACATAAAGAGCAGTGC27470.17667628620915204No Hit
GTGTATTACTGTGCAAAAGACTCAAGATACTGTGATAGTACCAGCTGCCA26420.16992309725685464No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT25690.16522802303287645No Hit
CCCTCAGTGTCCGTGTCCCCAGGACAGACAGCCAACATCACCTGCTCTGG25480.16387738524241696No Hit
CCCCAGGACAGACAGCCAACATCACCTGCTCTGGAGATAAGTTGGGGGAT25140.1616906383435778No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT24490.1575100928016794No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT24160.15538766198810025No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG23260.14959921431470247No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC22910.14734815133060333No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG22830.14683362264852354No Hit
GGCTATGGATGAGGCTGACTATTACTGTCAGGCGTGGGACAGCAGCACTGCTCTTTATGTCTTCGGACCGGGGAC22550.14503277226124422No Hit
AGCCAGGCCAGTCCCCTGTGCTGGTCATCTATCAAGATACCAAGCGGCCC21810.14027338195200606No Hit
TCCATAGCCTGGCTCCCGCTGATGGTCAGAGTGGCTGTGTTCCCAGAATTGGAGCCAGAGAATCGCTCAGGGATC21800.14020906586674609No Hit
GTAATAGTCAGCCTCATCCATAGCCTGGCTCCCGCTGATGGTCAGAGTGG21720.13969453718466626No Hit
CAGCCACTCTGACCATCAGCGGGAGCCAGGCTATGGATGAGGCTGACTATTACTGTCAGGCGTGGGACAGCAGCA21330.13718620985952723No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG21260.1367359972627074No Hit
GAGTGGCTGTGTTCCCAGAATTGGAGCCAGAGAATCGCTCAGGGATCCCT21220.1364787329216675No Hit
GGATCCGTGGCCTCCTATGAGCTGACTCAGCCACCCTCAGTGTCCGTGTCCCCAGGACAGACAGCCAACATCAC21160.13609283641010766No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG21150.1360285203248477No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG21140.13596420423958772No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA20030.12882511877573044No Hit
CAGTAATAGTCAGCCTCATCCATAGCCTGGCTCCCGCTGATGGTCAGAGT19520.12554499842747172No Hit
GGAGATAAGTTGGGGGATAAATATGCTTGTTGGTATCAGCAGAAGCCAGGCCAGTCCCCTGTGCTGGTCATCTAT19390.12470888931909205No Hit
GGCCAGTCCCCTGTGCTGGTCATCTATCAAGATACCAAGCGGCCCTCAGG18830.12110718854453342No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAATTCTGGGAACACAG18740.12052834377719365No Hit
ATCAAGATACCAAGCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAATTCTGGGAACACAGCCACTCTGA18540.11924202207199415No Hit
GTGCTGCTGTCCCACGCCTGACAGTAATAGTCAGCCTCATCCATAGCCTGGCTCCCGCTGATGGTCAGAGTGG18160.11679801083211508No Hit
GCATCGGAGGTGCCTCAGCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGGATCCGTGG17660.11358220656911631No Hit
GTGCTGGTCATCTATCAAGATACCAAGCGGCCCTCAGGGATCCCTGAGCG17530.11274609746073665No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC17440.11216725269339686No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC17380.11178135618183702No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC17310.11133114358501718No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT16550.10644312110525905No Hit
CAGCAGCACTGCTCTTTATGTCTTCGGACCGGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCA16340.10509248331479959No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG16250.1045136385474598No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA16240.10444932246219983No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA15740.10123351819920107No Hit
GGATGAGGCTGACTATTACTGTCAGGCGTGGGACAGCAGCACTGCTCTTTATGTCTTCGGACCGGGGACCAAGGT15710.10104056994342113No Hit
GCTCTTTATGTCTTCGGACCGGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACT15640.10059035734660132No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)