FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689153

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689153
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1064490
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGTTCCCTCACTTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA43320.40695544345179385No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31530.2961981794098582No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28100.2639761763849355No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCCGCTGATTGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA28000.26303675938712434No Hit
CTCCTGTAGGGCCAGTCAGAGTGTTAGCAGCAGCTACTTAGCCTGGTTCC23370.21954175238846774No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20910.1964320942423132No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA20610.19361384324887973No Hit
CATCTATGGTGCGTCCAACAGGGCCACTGGCATCTCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA20360.19126530075435186No Hit
GTGTATTACTGTCAGCAGTATGTTCCCTCACTTCCGTACACTTTTGGCCA18350.17238301909834758No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18200.17097389360163082No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18040.16947082640513297No Hit
CACTAATACTGACGGGAGTGTAACAATCTATGCGGACTCCGTGAAGGGCCGATTCACCACCTCCAGAGACAACGC17810.16731016731016732No Hit
CCATAGATGAGGAGCCTGGGAACCTGGCCAGGTTTCTGCTGGAACCAGGCTAAGTAGCTGCTGCTAACACTCTGA17450.16392826611804714No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16600.15594322163665228No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG16470.1547219795394978No Hit
GTCTGGGACAGACTTCACTCTCACAATCAGCGGACTGGAGCCTGAAGATT16360.15368862084190552No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16170.15190372854606432No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15970.150024894550442No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGTAGGGCCAGTCAGAGTGTTAGCAG15830.14870971075350636No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14940.14034889947298707No Hit
TCTATGGTGCGTCCAACAGGGCCACTGGCATCTCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT14840.1394094824751759No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT14720.13828218207780252No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14600.13715488168042914No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14270.13405480558765231No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCCGCTGATTGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA14220.13358509708874672No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGAGATGCCAGTGGCCCTGTT13460.1264455279053819No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13240.12437881051019738No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC13020.12231209311501282No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT12800.12024537571982828No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12690.119212017022236No Hit
ATAATACACAGCCGTGTCCTCGGCTGTCAGACTGTTCATTTGCAAATACA12530.11770894982573814No Hit
GATCAGGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGG12480.11723924132683258No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12440.11686347452770811No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12260.11517252393164802No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGTAGGGCCAGTCAGA12200.11460887373296133No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12110.11376339843493129No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12000.112730039737339No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11950.11226033123843343No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA11910.11188456443930897No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGT11710.11000573044368665No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11600.10897237174609437No Hit
CAATAATACACAGCCGTGTCCTCGGCTGTCAGACTGTTCATTTGCAAATA11560.1085966049469699No Hit
GTCAGAGTGTTAGCAGCAGCTACTTAGCCTGGTTCCAGCAGAAACCTGGC11500.10803295474828321No Hit
GTATGTTCCCTCACTTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT11470.10775112964893988No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC11410.10718747945025317No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG11320.10634200415222313No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACGGAAGTGAGGGAACATACTG11130.10455711185638193No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT11100.10427528675703858No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAATTATCACTGGATGCATTGGGTCCGCCAAGCTCCAGGGGAGGGG10710.10061156046557507No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGC200.00827256650.240313
CGCGTAT1800.045.77842739
ATCGCGT2100.045.5437737
GTCGTAT405.146239E-542.9172839
GGTATCA7550.042.5998231
TATTCCG503.6719484E-641.6741843
CGTATGC4800.040.2655341
ATGGGGG3250.040.17683870
GCGTATG1900.039.85474840
TATACAC12200.039.8078963
CCGTCGT801.3860699E-939.4849547
CTTATAC12050.039.202661
TCGCGTA2100.039.12936838
TTATACA12250.039.0985832
GTATGCC5800.038.8158742
ATGCCGG1800.038.68518444
GTATCAA19850.037.8068921
CATCGCG3200.037.26194436
ACGAGAC12900.036.8965922
CACGAGA12900.036.88965221
Produced by FastQC (version 0.11.5)