FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689154

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689154
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1064490
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG121401.1404522353427462No Hit
GTATTACTGTCAGCAGTATGTTCCCTCACTTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA41700.39173688808725304No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32330.3037135153923475No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCCGCTGATTGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA26460.2485697376208325No Hit
CTCCTGTAGGGCCAGTCAGAGTGTTAGCAGCAGCTACTTAGCCTGGTTCC22360.21005364071057503No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21370.20075341243224457No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20810.1954926772445021No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19350.18177718907645915No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA19110.1795225882817124No Hit
GTGTATTACTGTCAGCAGTATGTTCCCTCACTTCCGTACACTTTTGGCCA18390.17275878589747204No Hit
CATCTATGGTGCGTCCAACAGGGCCACTGGCATCTCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA18260.17153754380031752No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17560.16496162481563942No Hit
GTCTGGGACAGACTTCACTCTCACAATCAGCGGACTGGAGCCTGAAGATT16760.15744628883315015No Hit
CACTAATACTGACGGGAGTGTAACAATCTATGCGGACTCCGTGAAGGGCCGATTCACCACCTCCAGAGACAACGC16690.15678869693468234No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16420.15425227104059222No Hit
CCATAGATGAGGAGCCTGGGAACCTGGCCAGGTTTCTGCTGGAACCAGGCTAAGTAGCTGCTGCTAACACTCTGA16080.15105825324803426No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGTAGGGCCAGTCAGAGTGTTAGCAG15340.1441065674642317No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14810.13912765737583258No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14700.13809429867824027No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCCGCTGATTGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA14360.13490028088568234No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGAGATGCCAGTGGCCCTGTT14140.1328335634904978No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13560.12738494490319308No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13500.12682129470450637No Hit
TCTATGGTGCGTCCAACAGGGCCACTGGCATCTCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT13430.12616370280603859No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC13320.1251303441084463No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13030.12240603481479394No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG12840.12062114251895274No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGTAGGGCCAGTCAGA12570.1180847166248626No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12470.11714529962705146No Hit
ATAATACACAGCCGTGTCCTCGGCTGTCAGACTGTTCATTTGCAAATACA12430.11676953282792699No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACGGAAGTGAGGGAACATACTG12150.11413916523405575No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12130.1139512818344935No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11880.1116027393399656No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11830.11113303084106005No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA11790.11075726404193559No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT11750.11038149724281111No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11690.10981784704412441No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11640.10934813854521883No Hit
CAATAATACACAGCCGTGTCCTCGGCTGTCAGACTGTTCATTTGCAAATA11290.10606017905287977No Hit
GATCAGGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGG11120.10446317015660081No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGT11050.103805578258133No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAATTATCACTGGATGCATTGGGTCCGCCAAGCTCCAGGGGAGGGG10900.10239645276141626No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10840.10183280256272956No Hit
GGCCAAAAGTGTACGGAAGTGAGGGAACATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCCG10840.10183280256272956No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC10830.10173886086294846No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10800.1014570357636051No Hit
GTATGTTCCCTCACTTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT10730.1007994438651373No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC10700.10051761876579396No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10660.10014185196666948No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)