FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689155

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689155
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences396439
Sequences flagged as poor quality0
Sequence length21-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACAGCCGTGTCCTCGTCTCTCAGGCTGTCCATTTGCAGATACA14610.36853084585522616No Hit
AATTATTACTGTGCAGCATGGGATGACAGCCTGAATGGTTGGGTGTTCGG13760.34708996844407336No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC12540.31631600321865405No Hit
GTAATAATTAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG11910.30042452937274083No Hit
GTATATTACTGTGTGGGACGATCCGTGGGACCGTTTGAATACTGGGGCCA11730.29588410827390843No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCCCCGCCGAACACCCAACCATTCAGGCTGTCATCCCATG10500.26485789743188737No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG9060.22853452864122853No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT8790.22172389699298No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG8410.2121385635621117No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACTC8380.21138182671230632No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG8000.201796493281438No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA7560.19069768615095892No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA7330.18489603696911758No Hit
CAGTAATATACAGCCGTGTCCTCGTCTCTCAGGCTGTCCATTTGCAGATA7270.18338256326950678No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT7110.17934663340387802No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG6610.16673435257378816No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT6600.16648210695718635No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCCC6580.16597761572398276No Hit
GAGTTGGGGCTGTGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGT6300.15891473845913243No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT6280.15841024722592884No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC6280.15841024722592884No Hit
GACCTAGGACGGTCAGCTTGGTCCCCCCGCCGAACACCCAACCATTCAGG6230.15714901914291984No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG6230.15714901914291984No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT6220.15689677352631806No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC6200.15639228229311447No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG6140.15487880859350367No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTGGGGCTGTGCTGGGTTTTC6100.1538698261270965No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG6010.1515996155776803No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT5850.14756368571205153No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT5810.14655470324564435No Hit
GCCCTGGGAGTTGCTGGTACCAGTTTACAGAATTAGTTCCGATGTTGGAACTGCTTCCAGAACAAGAGAAGGTGA5670.14302326461321918No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG5660.1427710189966174No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG5660.1427710189966174No Hit
GTATAGATGAGGAGTTTGGGGGCCAGCCCTGGGAGTTGCTGGTACCAGTTTACAGAATTAGTTCCGATGTTGGAA5560.1402485628305994No Hit
CTCCTGTGCAGCCTCTGGATTCATCTTTAGAAAATATACCATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGG5460.13772610666458143No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC5310.13394242241555446No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG5210.1314199662495365No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG5140.12965424693332392No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC5070.12788852761711134No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC5020.12662729953410234No Hit
GTCTGAGGATGAGGCTAATTATTACTGTGCAGCATGGGATGACAGCCTGAATGGTTGGGTGTTCGGCGGGGGGAC5010.12637505391750056No Hit
TCCTCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACC4990.12587056268429697No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA4980.12561831706769516No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC4950.12486158021788975No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACTCCCGGGCAGAGGGTCAC4930.12435708898468616No Hit
GCATGGGATGACAGCCTGAATGGTTGGGTGTTCGGCGGGGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG4810.12133014158546461No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA4780.12057340473565921No Hit
ATATTACTGTGTGGGACGATCCGTGGGACCGTTTGAATACTGGGGCCAGG4760.12006891350245562No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC4700.11855543980284482No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT4690.11830319418624302No Hit
GTGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGC4670.11779870295303943No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC4670.11779870295303943No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG4650.11729421171983584No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC4630.11678972048663225No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA4610.11628522925342866No Hit
ACCATATATTACGCAGACTCTGTGAAGGGCCGCTTCACCATCTTCAGAGACAATGACAAGGACTCACTGTATCTG4610.11628522925342866No Hit
CATCTATACTAATGATCATAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCAC4580.11552849240362327No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC4570.11527624678702147No Hit
GTAATATACAGCCGTGTCCTCGTCTCTCAGGCTGTCCATTTGCAGATACAGTGAGTCCTTGTCATTGTCTCTGAA4540.11451950993721606No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC4500.11351052747080886No Hit
CCCCTGAGGGCCGCTGATTATTATGATCATTAGTATAGATGAGGAGTTTG4490.11325828185420707No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG4480.11300603623760527No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA4430.1117448081545963No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC4400.11098807130479091No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG4380.11048358007158732No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT4350.1097268432217819No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG4330.10922235198857831No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT4300.10846561513877293No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTAATTATTACTGTGCAG4230.10669989582256034No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT4220.10644765020595855No Hit
CCCTCAGCGTCTGGGACTCCCGGGCAGAGGGTCACCTTCTCTTGTTCTGG4200.10594315897275496No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT4200.10594315897275496No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC4170.10518642212294957No Hit
ATACTAATGATCATAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG4160.10493417650634777No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC4150.10468193088974598No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG4150.10468193088974598No Hit
CATTAGTATAGATGAGGAGTTTGGGGGCCAGCCCTGGGAGTTGCTGGTAC4130.10417743965654236No Hit
ATCCCATGCTGCACAGTAATAATTAGCCTCATCCTCAGACTGGAGCCCAC4120.10392519403994056No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC4090.10316845719013518No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT4090.10316845719013518No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCCCCGCCGAACAC3990.10064600102411721No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC3980.10039375540751541No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACATG150.002382903868.7909233
GCCCATC150.002409309868.600027
AGGGGGG755.3459735E-755.08847870
TTCCCGA200.00739227551.6847136
AGGTAAC200.007526517451.450027
CTTATAG200.007526517451.450022
AAAGGGG459.209489E-750.9304768
TCTCGTA352.2751816E-549.26723538
GATCTCG451.8744977E-645.94196736
CTAGATC451.8975243E-645.86061533
ACTAGAT451.9074669E-645.82583632
TACTAGA451.9091276E-645.82004531
CGTACTA451.915785E-645.79689829
ACCGTAC451.920791E-645.7795527
GACCGTA451.9224626E-645.7737726
GGTATCA2650.044.018671
ATGCCGT404.9067272E-543.2569544
TCGTATG404.9697213E-543.1635740
TCTGAAG5300.042.8754770
GTGGTAT903.6379788E-1241.932821
Produced by FastQC (version 0.11.5)