FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689156

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689156
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences396439
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATTATTACTGTGCAGCATGGGATGACAGCCTGAATGGTTGGGTGTTCGG14030.3539006000923219No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC12960.32691031911592955No Hit
GTAATATACAGCCGTGTCCTCGTCTCTCAGGCTGTCCATTTGCAGATACA12100.305217196088175No Hit
GTATATTACTGTGTGGGACGATCCGTGGGACCGTTTGAATACTGGGGCCA10980.27696568702877367No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACTC9240.2330749497400609No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG8490.21415652849492606No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCCCCGCCGAACACCCAACCATTCAGGCTGTCATCCCATG8340.21037284424589914No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT8280.2088593705462883No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG7960.20078751081503082No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT7790.19649933533280026No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7470.18842747560154274No Hit
GTAATAATTAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG7290.18388705450271037No Hit
CAGTAATATACAGCCGTGTCCTCGTCTCTCAGGCTGTCCATTTGCAGATA7240.1826258264197014No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA7050.17783315970426725No Hit
GAGTTGGGGCTGTGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGT6360.1604282121587432No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG6310.1591669840757342No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACTCCCGGGCAGAGGGTCAC6310.1591669840757342No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT6190.15614003667651266No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT5920.1493294050282641No Hit
CTCCTGTGCAGCCTCTGGATTCATCTTTAGAAAATATACCATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGG5900.14882491379506052No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC5840.14731144009544975No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA5750.14504122954603355No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC5680.143275510229821No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG5670.14302326461321918No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG5620.1417620365302102No Hit
CATCTATACTAATGATCATAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCAC5590.14100529968040482No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG5540.1397440715973958No Hit
GTAATATACAGCCGTGTCCTCGTCTCTCAGGCTGTCCATTTGCAGATACAGTGAGTCCTTGTCATTGTCTCTGAA5530.13949182598079402No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCCC5530.13949182598079402No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG5520.1392395803641922No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC5510.13898733474759042No Hit
GTATAGATGAGGAGTTTGGGGGCCAGCCCTGGGAGTTGCTGGTACCAGTTTACAGAATTAGTTCCGATGTTGGAA5490.13848284351438683No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC5440.13722161543137784No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC5430.13696936981477606No Hit
GCCCTGGGAGTTGCTGGTACCAGTTTACAGAATTAGTTCCGATGTTGGAACTGCTTCCAGAACAAGAGAAGGTGA5400.13621263296497066No Hit
GCATGGGATGACAGCCTGAATGGTTGGGTGTTCGGCGGGGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG5370.13545589611516526No Hit
GTCTGAGGATGAGGCTAATTATTACTGTGCAGCATGGGATGACAGCCTGAATGGTTGGGTGTTCGGCGGGGGGAC5260.1326811943325455No Hit
ACCATATATTACGCAGACTCTGTGAAGGGCCGCTTCACCATCTTCAGAGACAATGACAAGGACTCACTGTATCTG5260.1326811943325455No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG5150.1299064925499257No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG5090.12839301885031493No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT5070.12788852761711134No Hit
ATACTAATGATCATAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG5050.12738403638390774No Hit
GTGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGC5040.12713179076730594No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG5040.12713179076730594No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG4950.12486158021788975No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA4850.12233912405187179No Hit
GACCTAGGACGGTCAGCTTGGTCCCCCCGCCGAACACCCAACCATTCAGG4850.12233912405187179No Hit
ATATTACTGTGTGGGACGATCCGTGGGACCGTTTGAATACTGGGGCCAGG4780.12057340473565921No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG4740.11956442226925201No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC4700.11855543980284482No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA4670.11779870295303943No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT4620.11653747487003045No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC4610.11628522925342866No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT4600.11603298363682686No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCCCCGCCGAACAC4460.11250154500440168No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT4400.11098807130479091No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG4390.11073582568818911No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC4370.11023133445498552No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA4320.10897010637197652No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC4310.10871786075537472No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA4250.10720438705576393No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTGGGGCTGTGCTGGGTTTTC4220.10644765020595855No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTAATTATTACTGTGCAG4170.10518642212294957No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC4140.10442968527314415No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC4090.10316845719013518No Hit
CATTAGTATAGATGAGGAGTTTGGGGGCCAGCCCTGGGAGTTGCTGGTAC4090.10316845719013518No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA4040.10190722910712618No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC4030.10165498349052439No Hit
ATATATGGTAGAACTACTACCAGTAATGTATGAAACCCACTGCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGTT4030.10165498349052439No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT3990.10064600102411721No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG3980.10039375540751541No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)