FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689157

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689157
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences674783
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACTTG25340.3755281327478612No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC23650.35048304417864706No Hit
GTCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGATCCCGTCCCCCTTGGT22760.33729361883746334No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACTTGAAAGCCAGTGCCCCACGTCTGACAGTAATAGTCAG18870.2796454563911658No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG18230.2701609258087415No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT16900.250450885692141No Hit
TCTTTAGACTGGAGGCTGGAGATGGTGAGGTAGCGCTCAGTCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGATC16520.24481944565882663No Hit
GACTATTACTGTCAGACGTGGGGCACTGGCTTTCAAGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG15520.2299998666237887No Hit
GCTCTGGGACTGAGCGCTACCTCACCATCTCCAGCCTCCAGTCTAAAGAT15170.2248130139615254No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA14990.22214548973521858No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC14950.22155270657381707No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG14930.22125631499311632No Hit
GTCTAAAGATGAGGCTGACTATTACTGTCAGACGTGGGGCACTGGCTTTCAAGTGTTCGGCGGAGGGACCAAG14770.21888518234751023No Hit
GGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCAC13840.20510297384492496No Hit
CTGTTAAGCTTCATCAAGTACCGAGGGCCCTTCTCTGGCTGCTGCTGATGCCATGCGATGGCGTAGCTGCTGTGC13410.19873055485985866No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT13350.19784138011775637No Hit
GCTTAACAGTGATGGCAGCCTCACCAAGGGGGACGGGATCCCTGATCGCT13260.19650761800460298No Hit
GTAATAGTCAGCCTCATCTTTAGACTGGAGGCTGGAGATGGTGAGGTAGC12950.1919135485037412No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC12600.1867266958414779No Hit
GTACTTGATGAAGCTTAACAGTGATGGCAGCCTCACCAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCTCCAG12410.18391097582482072No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA12400.18376278003447033No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG12310.18242901792131694No Hit
GCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCT12280.18198443055026578No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG12270.18183623475991542No Hit
CTTGAAAGCCAGTGCCCCACGTCTGACAGTAATAGTCAGCCTCATCTTTA12140.17990968948536049No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC12090.17916871053360858No Hit
CTATTACTGTCAGACGTGGGGCACTGGCTTTCAAGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCC11980.17753855683975442No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG11950.17709396946870326No Hit
CCTGCACTCTGAGCAGTGGGCACAGCAGCTACGCCATCGCATGGCATCAG11630.17235170417749113No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11490.17027696311258583No Hit
GTAATAAACGGCCGTGTCCTCGGCTCTCAGGCTGGTCATTTGCAGATACA11380.16864680941873167No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11260.1668684599345271No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11180.16568289361172409No Hit
CACCAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCTCCAGCTCTGGGACTGAGCGCTACCTCACCATCTCCAG11130.16494191465997218No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG11090.16434913149857067No Hit
CACCTGCACTCTGAGCAGTGGGCACAGCAGCTACGCCATCGCATGGCATC11090.16434913149857067No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC11010.16316356517576763No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA10770.1596068662073585No Hit
GCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAG10650.15782851672315396No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT10510.15575377565824863No Hit
CAGTAATAGTCAGCCTCATCTTTAGACTGGAGGCTGGAGATGGTGAGGTAGCGCTCAGTCCCAGAGCTGGAGC10260.15204888089948917No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA10170.15071511878633576No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG9880.14641744086617475No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC9840.14582465770477324No Hit
CCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGC9370.13885945555830542No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC9350.13856306397760465No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC9130.1353027565898963No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT8950.13263523236358948No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC8910.13204244920218797No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACTTGAAAGCCAGTGCCCCACGTCTGACAGTAATAGTCAGC8750.1296713165565819No Hit
CTCTGGGACTGAGCGCTACCTCACCATCTCCAGCCTCCAGTCTAAAGATG8620.12774477128202696No Hit
CCACTGCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGT8490.12581822600747203No Hit
CTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCACCTGCACT8450.1252254428460705No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACA8390.12433626810396824No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT8330.12344709336186598No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA8180.12122415650661028No Hit
GTTTATTACTGTGCGAAAGGAACAACAGCAGCCCCTGGGGCCTGTAGGTA8020.1188530238610042No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG7970.11811204490925231No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC7940.11766745753820117No Hit
GGCTTTCAAGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACT7900.11707467437679965No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG7670.11366617119874094No Hit
GTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCA7550.11188782171453637No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG7450.11040586381103258No Hit
ATCAAGTACCGAGGGCCCTTCTCTGGCTGCTGCTGATGCCATGCGATGGC7430.11010947223033182No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC7370.10922029748822956No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA7370.10922029748822956No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGTAGCTATGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG7340.1087757101171784No Hit
GAGCGCTACCTCACCATCTCCAGCCTCCAGTCTAAAGATGAGGCTGACTATTACTGTCAGACGTGGGGCACTGG7320.10847931853647766No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT7280.10788653537507614No Hit
CTACCTCACCATCTCCAGCCTCCAGTCTAAAGATGAGGCTGACTATTACT7250.107441948004025No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC7200.10670096905227311No Hit
CTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGC7080.10492261956806856No Hit
GCTTGGTCCCTCCGCCGAACACTTGAAAGCCAGTGCCCCACGTCTGACAGTAATAGTCAGCCTCATCTTTAGA7070.10477442377771816No Hit
GGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGT7020.10403344482596627No Hit
ATACTACACAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAACT6960.103144270083864No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT6940.10284787850316324No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT6910.1024032911321121No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG6870.10181050797071058No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCC150.002477622168.123235
ATCTCGT800.051.49307337
TACTCAC200.007588466651.34735533
GTTTACT200.00766246851.22147830
TCGTATG1150.050.92936740
CGTCTTC1100.050.62072448
GCCGTCT1100.050.49075346
GGTATCA3300.048.5299761
TCTCGTA850.048.5220538
AGGGGGG1551.2732926E-1147.14718670
GTGGTAT800.046.852081
CCGTCTT1200.046.34969347
CGTATGC1200.046.001841
ACGAGAC1350.045.42222222
TATACAC1300.044.542113
ATGCCGT1250.044.3247544
GTATGCC1250.044.2447542
GACAGGT705.002221E-1043.809741
CACGAGA1400.043.79999521
ACAGATC405.3798918E-542.59281
Produced by FastQC (version 0.11.5)