EGAF00004689158 FastQC Report

Basic Statistics

Measure	Value
Filename	EGAF00004689158
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	674783
Sequences flagged as poor quality	0
Sequence length	20-76
%GC	57

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

$Per base sequence content$

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCAC	2210	0.32751269667433824	No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC	2162	0.3203992987375201	No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACTTG	2147	0.31817636188226434	No Hit
GTCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGATCCCGTCCCCCTTGGT	1996	0.2957987975393571	No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG	1959	0.290315553296393	No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT	1558	0.23088904136589097	No Hit
GCTCTGGGACTGAGCGCTACCTCACCATCTCCAGCCTCCAGTCTAAAGAT	1544	0.22881430030098565	No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACTTGAAAGCCAGTGCCCCACGTCTGACAGTAATAGTCAG	1518	0.2249612097518758	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	1516	0.224664818171175	No Hit
TCTTTAGACTGGAGGCTGGAGATGGTGAGGTAGCGCTCAGTCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGATC	1502	0.2225900771062697	No Hit
GACTATTACTGTCAGACGTGGGGCACTGGCTTTCAAGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG	1421	0.210586218087889	No Hit
GTCTAAAGATGAGGCTGACTATTACTGTCAGACGTGGGGCACTGGCTTTCAAGTGTTCGGCGGAGGGACCAAG	1406	0.2083632812326333	No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG	1383	0.20495477805457457	No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA	1364	0.20213905803791737	No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG	1342	0.19887875065020905	No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG	1321	0.19576663905285108	No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC	1311	0.19428468114934727	No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT	1254	0.18583752109937565	No Hit
GTACTTGATGAAGCTTAACAGTGATGGCAGCCTCACCAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCTCCAG	1248	0.18494834635727336	No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG	1235	0.18302180108271843	No Hit
GCTTAACAGTGATGGCAGCCTCACCAAGGGGGACGGGATCCCTGATCGCT	1227	0.18183623475991542	No Hit
CTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCACCTGCACT	1222	0.18109525580816352	No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC	1219	0.1806506684371124	No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC	1214	0.17990968948536049	No Hit
GTAATAGTCAGCCTCATCTTTAGACTGGAGGCTGGAGATGGTGAGGTAGC	1209	0.17916871053360858	No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA	1206	0.17872412316255742	No Hit
GCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCT	1203	0.1782795357915063	No Hit
CTGTTAAGCTTCATCAAGTACCGAGGGCCCTTCTCTGGCTGCTGCTGATGCCATGCGATGGCGTAGCTGCTGTGC	1125	0.1667202641441767	No Hit
CCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGC	1114	0.16509011045032254	No Hit
CCTGCACTCTGAGCAGTGGGCACAGCAGCTACGCCATCGCATGGCATCAG	1111	0.1646455230792714	No Hit
GCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAG	1105	0.16375634833716912	No Hit
CACCTGCACTCTGAGCAGTGGGCACAGCAGCTACGCCATCGCATGGCATC	1093	0.16197799885296457	No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG	1077	0.1596068662073585	No Hit
CTATTACTGTCAGACGTGGGGCACTGGCTTTCAAGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCC	1074	0.15916227883630737	No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG	1059	0.1569393419810517	No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC	1053	0.1560501672389494	No Hit
CACCAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCTCCAGCTCTGGGACTGAGCGCTACCTCACCATCTCCAG	1048	0.1553091882871975	No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA	1033	0.15308625143194182	No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT	1032	0.15293805564159146	No Hit
CCACTGCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGT	1027	0.15219707668983956	No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG	1019	0.1510115103670365	No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACA	982	0.14552826612407246	No Hit
GTAATAAACGGCCGTGTCCTCGGCTCTCAGGCTGGTCATTTGCAGATACA	981	0.1453800703337221	No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA	961	0.14241615452671452	No Hit
CAGTAATAGTCAGCCTCATCTTTAGACTGGAGGCTGGAGATGGTGAGGTAGCGCTCAGTCCCAGAGCTGGAGC	937	0.13885945555830542	No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT	927	0.13737749765480162	No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA	910	0.13485816921884516	No Hit
CTTGAAAGCCAGTGCCCCACGTCTGACAGTAATAGTCAGCCTCATCTTTA	909	0.1347099734284948	No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT	888	0.13159786183113684	No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC	871	0.12907853339518038	No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG	869	0.1287821418144796	No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC	865	0.1281893586530781	No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT	865	0.1281893586530781	No Hit
CTCTGGGACTGAGCGCTACCTCACCATCTCCAGCCTCCAGTCTAAAGATG	865	0.1281893586530781	No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC	864	0.1280411628627277	No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC	843	0.12492905126536977	No Hit
GTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCA	831	0.1231507017811652	No Hit
GGCTTTCAAGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACT	821	0.12166874387766141	No Hit
GGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGT	764	0.11322158382768979	No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC	764	0.11322158382768979	No Hit
GAGCGCTACCTCACCATCTCCAGCCTCCAGTCTAAAGATGAGGCTGACTATTACTGTCAGACGTGGGGCACTGG	750	0.11114684276278448	No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA	748	0.11085045118208373	No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGTAGCTATGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG	747	0.11070225539173335	No Hit
CCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCACCTGCACTCT	743	0.11010947223033182	No Hit
GTTTATTACTGTGCGAAAGGAACAACAGCAGCCCCTGGGGCCTGTAGGTA	740	0.10966488485928069	No Hit
CTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGC	732	0.10847931853647766	No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT	729	0.1080347311654265	No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC	725	0.107441948004025	No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC	715	0.1059599901005212	No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT	708	0.10492261956806856	No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACTTGAAAGCCAGTGCCCCACGTCTGACAGTAATAGTCAGC	702	0.10403344482596627	No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA	696	0.103144270083864	No Hit
TCCTCACCCTCCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCT	687	0.10181050797071058	No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG	683	0.10121772480930907	No Hit
GAGTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTG	681	0.10092133322860831	No Hit
GTATCAACGCAGAGTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTC	678	0.10047674585755717	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCATGT	30	0.0015940357	76.12413	70
CGCAAAG	20	0.007761053	51.05611	8
AGTAGTT	65	5.762723E-4	46.84562	70
GGTATCA	505	0.0	43.817028	1
GTATCAA	1125	0.0	36.614555	1
GAGTATA	40	0.0021285606	35.923344	64
GACAGCC	105	1.8189894E-11	35.66353	1
GTGGTAT	115	1.8189894E-12	35.522564	1
TCGAACG	40	0.0024733543	34.840874	49
TCTGGAG	620	0.0	34.37864	70
GACAGGT	90	5.9044396E-9	34.04246	1
ACGGGAA	50	2.0133049E-4	34.039932	5
TGGTATC	130	7.2759576E-12	31.421476	2
GACAGGA	120	9.094947E-11	31.205585	1
GATTGAC	55	3.5206808E-4	30.94769	1
TAAGCTA	45	0.004556796	30.75491	43
TATCAAC	1330	0.0	30.71272	2
AATGGTA	125	3.8668493E-4	30.449652	70
GGGCTAC	45	0.0049331854	30.259966	1
ATCAACG	1350	0.0	30.003345	3

Summary