FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689159

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689159
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences553291
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23350.42202023889779516No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22700.4102723521618823No Hit
GAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGATCTATAAGGCCT22190.40105477949216595No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18270.3302059856386603No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT16660.30110737387739905No Hit
CTATAGTAATTACCTCATGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT16060.29026317073655633No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15480.2797804410337417No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15410.2785152840006434No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA14540.2627911894464215No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14360.2595379285041687No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13960.2523084597436069No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13880.25086256599149453No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13360.24146425660276416No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG12800.23134300033797764No Hit
TTACTATAGTGTTGGCAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCCGCTGATGGTGAGAGTGAAGTCTGTC12760.2306200534619215No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12480.22555942532952825No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC12420.22447500501544393No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12370.2235713214203737No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12040.21760700969291025No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11520.20820870030417987No Hit
GACTTCACTCTCACCATCAGCGGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGCCAACACTATAGTAATTAC11350.20513617608094115No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAA11180.20206365185770234No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10910.19718376044432312No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG10370.1874239776175647No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA10350.18706250417953663No Hit
ACTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCGGTGGATCTGGGACAGACTTCACTCTCACCATCAGCGGC9920.17929082526193268No Hit
GAGTGAAGTCTGTCCCAGATCCACCGCCGCTGAACCTTGATGGGACCCCA9830.17766419479080628No Hit
GGCCAAAAGTGTACATGAGGTAATTACTATAGTGTTGGCAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCCG9660.17459167056756753No Hit
GTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGATCTATAAGGCCTCTA9390.1697117791541883No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9230.16681999164996358No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9090.16428967758376695No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8950.1617593635175703No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8880.16049420648447202No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT8860.1601327330464439No Hit
CTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA8800.15904831273235964No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8610.1556143150710928No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT8430.15236105412884No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8190.1480233728725029No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC8080.1460352689633484No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG7840.14169758770701132No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7820.14133611426898324No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC7810.14115537754996918No Hit
GTAATTACCTCATGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA7740.13989022051687086No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG7720.13952874707884277No Hit
GGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGCTACTA7660.1384443267647585No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT7570.1368176962936321No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7480.1351910658225057No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG7420.13410664550842144No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT7400.13374517207039333No Hit
CATGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGG7340.13266075175630906No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC7260.13121485800419672No Hit
ATCTGGGACAGACTTCACTCTCACCATCAGCGGCCTGCAGCCTGAAGATT7230.13067264784715457No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT7100.128323070499972No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG7060.12760012362391582No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA6990.1263349665908175No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC6900.1247083361196911No Hit
TTCTAAAGTAGAGGCCTTATAGATCAGGAGGTTAGGGGCTTTCCCTGGTT6670.12055139158236804No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG6630.11982844470631188No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC6580.11892476111124163No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA6380.11531002673096075No Hit
GCCCCTAACCTCCTGATCTATAAGGCCTCTACTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCGGTGGAT6360.11494855329293266No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT6360.11494855329293266No Hit
GTCCCAGATCCACCGCCGCTGAACCTTGATGGGACCCCACTTTCTAAAGT6350.11476781657391862No Hit
CACCATCAGCGGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGCCAACACTATAGTAATTACCTCATGTACA6300.11386413297884837No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAG6280.1135026595408203No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC6280.1135026595408203No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC6220.11241823922673601No Hit
GTGTACATGAGGTAATTACTATAGTGTTGGCAGTAATAAGTTGCAAAATC6210.11223750250772196No Hit
CTATAAGGCCTCTACTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCGGTGGATCTGGGACAGACTTCACTCT6210.11223750250772196No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA6190.11187602906969389No Hit
ACTTATTACTGCCAACACTATAGTAATTACCTCATGTACACTTTTGGCCA6160.11133381891265176No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA6150.11115308219363772No Hit
CATCAAGGTTCAGCGGCGGTGGATCTGGGACAGACTTCACTCTCACCATCAGCGGCCTGCAGCCTGAAGATTTTG6130.1107916087556096No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA6120.11061087203659556No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA6030.10898424156546915No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGATCTATAAGGCCTCTACTTTAGAAAGTGGGGTCCCAT6010.10862276812744108No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG5910.10681540093730063No Hit
GTAATAAGTTGCAAAATCTTCAGGCTGCAGGCCGCTGATGGTGAGAGTGAAGTCTGTCCCAGATCCACCGCCG5910.10681540093730063No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT5870.10609245406124446No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT5790.1046465603091321No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC5710.10320066655701973No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA5700.10301992983800569No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT5700.10301992983800569No Hit
ACACTATAGTAATTACCTCATGTACACTTTTGGCCAGGGGACCAAGCTGG5680.10265845639997757No Hit
ACTATAGTGTTGGCAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCCGC5680.10265845639997757No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)