FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689160

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689160
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences553291
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31700.5729353992745229No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23460.4240083428069497No Hit
GAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGATCTATAAGGCCT20790.3757516388301997No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18420.332917036423871No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17630.31863883562176143No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16180.2924320113647249No Hit
CTATAGTAATTACCTCATGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT14370.2597186652231827No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14130.25538098396684566No Hit
TTACTATAGTGTTGGCAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCCGCTGATGGTGAGAGTGAAGTCTGTC13750.24851298864431193No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13710.24779004176825578No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA13520.24435604410698888No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12960.23423478784220236No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12790.23116226361896364No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC12380.22375205813938778No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12250.2214024807922052No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11790.2130885917175591No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11670.2109197510893906No Hit
GACTTCACTCTCACCATCAGCGGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGCCAACACTATAGTAATTAC11630.21019680421333437No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11510.20802796358516587No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG11300.20423249248587091No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10620.19194239559291584No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAA10290.18597808386545236No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA10200.18435145339432596No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG10050.18164040260911526No Hit
GAGTGAAGTCTGTCCCAGATCCACCGCCGCTGAACCTTGATGGGACCCCA9860.17820640494784842No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG9780.17676051119573608No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9700.1753146174436237No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9580.17314577681545515No Hit
GGCCAAAAGTGTACATGAGGTAATTACTATAGTGTTGGCAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCCG9580.17314577681545515No Hit
GTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGATCTATAAGGCCTCTA9480.1713384096253147No Hit
ACTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCGGTGGATCTGGGACAGACTTCACTCTCACCATCAGCGGC9450.17079619946827257No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9020.16302452055066863No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT8940.16157862679855628No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8830.1595905228894018No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT8690.15706020882320515No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT8630.1559757885091209No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8180.14784263615348886No Hit
CTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA8090.14621600568236245No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA8050.14549305880630625No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8030.14513158536827817No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC7980.14422790177320796No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG7880.1424205345830675No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG7630.13790211660771637No Hit
GGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGCTACTA7420.13410664550842144No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG7370.1332029619133512No Hit
GTCCCAGATCCACCGCCGCTGAACCTTGATGGGACCCCACTTTCTAAAGT7320.13229927831828098No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7250.13103412128518266No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT7140.12904601737602817No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT7050.12741938690490176No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG6980.12615422987180344No Hit
ATCTGGGACAGACTTCACTCTCACCATCAGCGGCCTGCAGCCTGAAGATT6880.124346862681663No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA6720.12145507517743828No Hit
GTAATTACCTCATGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA6590.11910549783025567No Hit
TTCTAAAGTAGAGGCCTTATAGATCAGGAGGTTAGGGGCTTTCCCTGGTT6530.11802107751617143No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC6430.11621371032603098No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA6310.11404486969786241No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC6300.11386413297884837No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC6220.11241823922673601No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC6130.1107916087556096No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAG6110.11043013531758153No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC6090.11006866187955344No Hit
GCCCCTAACCTCCTGATCTATAAGGCCTCTACTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCGGTGGAT6080.1098879251605394No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT6030.10898424156546915No Hit
GTGTACATGAGGTAATTACTATAGTGTTGGCAGTAATAAGTTGCAAAATC6010.10862276812744108No Hit
CACCATCAGCGGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGCCAACACTATAGTAATTACCTCATGTACA6000.10844203140842704No Hit
ACTTATTACTGCCAACACTATAGTAATTACCTCATGTACACTTTTGGCCA5950.10753834781335679No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC5920.10699613765631467No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA5910.10681540093730063No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA5870.10609245406124446No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA5840.10555024390420231No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC5840.10555024390420231No Hit
CATGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGG5660.1022969829619495No Hit
GGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAGCAGCAGGAGCCCCAGGAG5630.10175477280490737No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA5570.1006703524908231No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG200.004081598560.05681268
GCCGTAT200.00534055556.114366
TGTACGA200.006253247653.9209857
GTATCAA14950.037.087241
ACGGGAC558.78434E-636.69795616
AGTCATT800.002022423836.29141670
TGTTAGA7300.031.8171370
CAGTTCG9200.031.5577570
TCAACGC18250.030.5707074
TAGTACC553.79619E-430.5512036
CGCAAGG553.8082816E-430.5346312
TATCAAC18250.030.3865472
ATCAACG18250.030.3865473
AACGCAG18450.030.2365826
CAACGCA18650.029.9123275
TTATCTA902.5051122E-729.8749874
GGTTATC902.5051122E-729.8749872
TCTTACA500.00584447929.22725762
AATGGTA1255.109005E-429.0331370
GGAAACG1000.006032384529.0331370
Produced by FastQC (version 0.11.5)