FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689163

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689163
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1100838
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACATGGAAAGAATTACCGCTTTCCG45450.4128672883748562No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC44720.4062359765923778No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT37780.343193094715117No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG33650.30567622120602667No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC31680.28778076338207803No Hit
CCCCAGGACAGACGGCCATGATCACCTGCTCTGGAGATGCATTGCCAACA31440.28560060608372895No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT28610.2598929179406961No Hit
GGCTCTGAGGCCTCCTATGAATTGACACAGCCACCCTCGGTGTCGGTGTCCCCAGGACAGACGGCCATGATCAC26750.24299669887849076No Hit
CTATTATTGTCAATCAGCGGAAAGCGGTAATTCTTTCCATGTGGTTTTCG26750.24299669887849076No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT25750.23391271013536963No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23660.2149271736622464No Hit
GTAATATATGGCCGTGTCCTCGGCTCTCAGACTGTTCAGTTGAAGATAGAGTGTGTTCTTGGATTTGTCCCTGGA23510.21356457535077825No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG22790.20702410345573102No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT22780.20693326356829977No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT21260.19312560067875564No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC21240.19294392090389323No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGGTCAGGCGTCAGGCTCAGATAG20830.18921948551921355No Hit
CCCTTAGGGATCCCTGAGCGATTCTCTGGCTCCAGCTCAGGGACAACAGC20710.188129406870039No Hit
GAATTACCGCTTTCCGCTGATTGACAATAATAGTCAGCCTCGTCTTCTGC20370.1850408506973778No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC20360.1849500108099466No Hit
GGTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA20150.18304237317389116No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG20090.1824973338493039No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACATGGAAAGAA19870.18049885632581725No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19640.17840953891489936No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC19280.17513930296737576No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA19020.17277746589416426No Hit
CCTATGAATTGACACAGCCACCCTCGGTGTCGGTGTCCCCAGGACAGACGGCCATGATCACCTGCTCTGGAGATG18800.17077898837067762No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC18610.1690530305094846No Hit
GTCCAGGAGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTAC18530.1683263114100349No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGGTCAGGCGTCAGGCTCAGATAGCTG18010.1636026372636119No Hit
CTCTACTTCTCCCCCTCTTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAATTGACACAGCCACCCTCGGTGT17740.1611499603029692No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA17600.15987820187893223No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA17480.1587881232297577No Hit
CAATAATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAAGGT17450.15851560356746405No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGGTCAGGCGTCAGGCTCAGATAGCTGCTGG17070.155063687845078No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC16090.1461613788768193No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC16030.14561633955223202No Hit
CTCCTGTGCAGTGTCTGAGTTTTCCGTCGACAGCATGGACATAACCTGGGTCCGCCAGGCTCCAGGGAAGGGG15780.14334534236645174No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC15490.14071098563094658No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT15360.13953006709434085No Hit
GGCCAGGCCCCTGTGGCGGTGATATATAAAGATACTGAGAGGCCCTTAGG15210.13816746878287267No Hit
ATATATTACTGTGCGAGAGTGAAGAGGGAGATACTTACGGGGGCCTGGTT15140.1375315895708542No Hit
CTGCACAGGCTCTGAGGCCTCCTATGAATTGACACAGCCACCCTCGGTGT15090.13707739013369816No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC14840.13480639294791785No Hit
GCTTGGTCCCTCCGCCGAAAACCACATGGAAAGAATTACCGCTTTCCGCTGATTGACAATAATAGTCAGCCTCGT14650.13308043508672485No Hit
CTCTTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAATTGACACAGCCACCCTCGGTGTCGGTGTCCCCAGGA14110.1281750811654394No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACATGGAAAGAATTACCGCTTTCCGCTGATTGACAAT13990.1270850025162649No Hit
GTCCTGGGGACACCGACACCGAGGGTGGCTGTGTCAATTCATAGGAGGCC13870.12599492386709032No Hit
GCTCTGGAGATGCATTGCCAACACAATATGTTTATTGGTATCAGCAGAAG13840.1257224042047967No Hit
CCCTAAGGGCCTCTCAGTATCTTTATATATCACCGCCACAGGGGCCTGGC13790.12526820476764064No Hit
CTCTCTGCACAGGCTCTGAGGCCTCCTATGAATTGACACAGCCACCCTCGGTGTCGGTGTCCCCAGGACAGACGG13750.12490484521791581No Hit
GACTATTATTGTCAATCAGCGGAAAGCGGTAATTCTTTCCATGTGGTTTTCGGCGGAGGGACCAAGCTGACCGTC13640.12390560645617248No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA13410.12181628904525461No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGGTCAGGCGTCAGGCTCAGATAGC13400.12172544915782342No Hit
CACAATATGTTTATTGGTATCAGCAGAAGCCAGGCCAGGCCCCTGTGGCGGTGATATATAAAGATACTGAGAGGC13120.11918193230974947No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACATGGAAAGAAT12720.11554833681250101No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12640.11482161771305133No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC12590.11436741827589526No Hit
GTGGAGTCCAGGCAGAAGACGAGGCTGACTATTATTGTCAATCAGCGGAA12470.11327733962672074No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG12440.1130048199644271No Hit
GTGTCGGTGTCCCCAGGACAGACGGCCATGATCACCTGCTCTGGAGATGC12300.11173306154039014No Hit
GGCCTGGCCTGGCTTCTGCTGATACCAATAAACATATTGTGTTGGCAATGCATCTCCAGAGCAGGTGATCATGGC12220.11100634244094044No Hit
TCAGTGGAGTCCAGGCAGAAGACGAGGCTGACTATTATTGTCAATCAGCG11860.10773610649341683No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC11750.1067368677316735No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11740.10664602784424229No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT11740.10664602784424229No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG11580.10519258964534291No Hit
AGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTACTTCTCCCCCTCTTCACTCTCTGCACAGGCT11560.10501090987048049No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC11390.10346663178414989No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG11390.10346663178414989No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT11360.10319411212185625No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG11330.10292159245956262No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT11280.10246739302240655No Hit
CCACTGATGGTCAAGGTGGCTGTTGTCCCTGAGCTGGAGCCAGAGAATCG11240.10210403347268172No Hit
AGCCAGGCCAGGCCCCTGTGGCGGTGATATATAAAGATACTGAGAGGCCC11180.10155899414809445No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG11180.10155899414809445No Hit
GCAGAAGCCAGGCCAGGCCCCTGTGGCGGTGATATATAAAGATACTGAGAGGCCCTTAGGGATCCCTGAGCGATT11150.10128647448580082No Hit
GACATAACCTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTCTC11060.10046891549891993No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG11030.10019639583662629No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)