FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004689164

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689164
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1100838
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG62280.5657508189215852No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC40580.3686282631958563No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACATGGAAAGAATTACCGCTTTCCG39830.3618152716385154No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG35700.324298398129425No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT32950.2993174290858419No Hit
GGCTCTGAGGCCTCCTATGAATTGACACAGCCACCCTCGGTGTCGGTGTCCCCAGGACAGACGGCCATGATCAC28640.2601654376029897No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC28180.255986802781154No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG27670.2513539685221622No Hit
CTATTATTGTCAATCAGCGGAAAGCGGTAATTCTTTCCATGTGGTTTTCG27120.24635777471344555No Hit
CCCCAGGACAGACGGCCATGATCACCTGCTCTGGAGATGCATTGCCAACA26830.24372341797794045No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT26150.23754630563261805No Hit
GTAATATATGGCCGTGTCCTCGGCTCTCAGACTGTTCAGTTGAAGATAGAGTGTGTTCTTGGATTTGTCCCTGGA25040.2274630781277536No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT23750.2157447326491273No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC21610.19630499673884802No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGGTCAGGCGTCAGGCTCAGATAG21580.1960324770765544No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21400.1943973591027926No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG20580.18694848833343325No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC19890.18068053610067966No Hit
GAATTACCGCTTTCCGCTGATTGACAATAATAGTCAGCCTCGTCTTCTGC19510.17722862037829362No Hit
CCCTTAGGGATCCCTGAGCGATTCTCTGGCTCCAGCTCAGGGACAACAGC19510.17722862037829362No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG18960.172232426569577No Hit
CTCTACTTCTCCCCCTCTTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAATTGACACAGCCACCCTCGGTGT18860.17132402769526486No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC18820.17096066814554003No Hit
GGTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA18600.1689621906220534No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT18290.16614615411168582No Hit
GTCCAGGAGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTAC18080.16423851647563037No Hit
CCTATGAATTGACACAGCCACCCTCGGTGTCGGTGTCCCCAGGACAGACGGCCATGATCACCTGCTCTGGAGATG17770.16142247996526282No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA17180.15606292660682136No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC17080.15515452773250923No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA17050.1548820080702156No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGGTCAGGCGTCAGGCTCAGATAGCTGCTGG16530.1501583339237926No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGGTCAGGCGTCAGGCTCAGATAGCTG16350.1485232159500308No Hit
CTCCTGTGCAGTGTCTGAGTTTTCCGTCGACAGCATGGACATAACCTGGGTCCGCCAGGCTCCAGGGAAGGGG15910.1445262609030575No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACATGGAAAGAA15770.14325450247902052No Hit
ATATATTACTGTGCGAGAGTGAAGAGGGAGATACTTACGGGGGCCTGGTT15700.14261862326700206No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC15630.14198274405498357No Hit
CTCTTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAATTGACACAGCCACCCTCGGTGTCGGTGTCCCCAGGA15160.13771326934571665No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA15060.13680487047140452No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA14780.13426135362333058No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC14520.1318995165501191No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC14440.1311727974506694No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACATGGAAAGAATTACCGCTTTCCGCTGATTGACAAT14440.1311727974506694No Hit
GACTATTATTGTCAATCAGCGGAAAGCGGTAATTCTTTCCATGTGGTTTTCGGCGGAGGGACCAAGCTGACCGTC14350.1303552384637885No Hit
GGCCAGGCCCCTGTGGCGGTGATATATAAAGATACTGAGAGGCCCTTAGG14240.12935599970204517No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT14210.12908348003975154No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC14180.1288109603774579No Hit
CTGCACAGGCTCTGAGGCCTCCTATGAATTGACACAGCCACCCTCGGTGT14160.12862928060259549No Hit
GCTTGGTCCCTCCGCCGAAAACCACATGGAAAGAATTACCGCTTTCCGCTGATTGACAATAATAGTCAGCCTCGT14040.12753920195342092No Hit
GCTCTGGAGATGCATTGCCAACACAATATGTTTATTGGTATCAGCAGAAG13980.12699416262883367No Hit
ATCTGAGCCTGACGCCTGACCAGTGGAAGTCCCACAGAAGCTACAGCTGC13940.12663080307910882No Hit
CTCTCTGCACAGGCTCTGAGGCCTCCTATGAATTGACACAGCCACCCTCGGTGTCGGTGTCCCCAGGACAGACGG13760.124995685105347No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGGTCAGGCGTCAGGCTCAGATAGC13760.124995685105347No Hit
CCCTAAGGGCCTCTCAGTATCTTTATATATCACCGCCACAGGGGCCTGGC13670.12417812611846611No Hit
CACAATATGTTTATTGGTATCAGCAGAAGCCAGGCCAGGCCCCTGTGGCGGTGATATATAAAGATACTGAGAGGC13390.12163460927039219No Hit
CAATAATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAAGGT13350.12127124972066736No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13230.1201811710714928No Hit
GTGGAGTCCAGGCAGAAGACGAGGCTGACTATTATTGTCAATCAGCGGAA13060.11863689298516221No Hit
GGCCTGGCCTGGCTTCTGCTGATACCAATAAACATATTGTGTTGGCAATGCATCTCCAGAGCAGGTGATCATGGC12740.11573001658736345No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG12630.11473077782562012No Hit
GTGTCGGTGTCCCCAGGACAGACGGCCATGATCACCTGCTCTGGAGATGC12540.11391321883873923No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC12530.11382237895130799No Hit
GTCCTGGGGACACCGACACCGAGGGTGGCTGTGTCAATTCATAGGAGGCC12080.10973458401690349No Hit
TCAGTGGAGTCCAGGCAGAAGACGAGGCTGACTATTATTGTCAATCAGCG11760.10682770761910472No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT11630.10564678908249897No Hit
CCACTGATGGTCAAGGTGGCTGTTGTCCCTGAGCTGGAGCCAGAGAATCG11560.10501090987048049No Hit
AGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTACTTCTCCCCCTCTTCACTCTCTGCACAGGCT11390.10346663178414989No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)