FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689167

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689167
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences719712
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTATTACTGTGCAGCATGGGATGACAGCCTGAATGGTTGGGTGTTCGG48490.6737417189097862No Hit
GTAATAAATAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG43280.6013516517718198No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC24750.3438875550220088No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACTC21370.2969243252856698No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCCAACCATTCAGGCTGTCATCCCATG21240.29511804721888757No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG20510.28497510115157176No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA18000.2501000400160064No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT17090.2374560935485305No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT16610.23078675914810368No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTC16400.22786892534791695No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG14930.20744408874660974No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT14830.20605464407985416No Hit
ATATTACTGTGTGGGACGATCCGTGGGACCGTTTGAGTACTGGGGCCAGG14150.19660642034591613No Hit
GATTAATAGTAGAGATGAGGAGTTTGGGGGCCATTCCTGGGAGTTGTTGG13720.1906318082788671No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC13430.1866024187452759No Hit
GTAATATAGAGCCGTGTCCTCGTCTCTCAGGTTGTTCATTTGCAGATACA13290.18465719621181806No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13140.18257302921168467No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG13100.18201725134498245No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT12940.1797941398781735No Hit
CAGTAATATAGAGCCGTGTCCTCGTCTCTCAGGTTGTTCATTTGCAGATA12910.17937730647814681No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC12800.17784891734471567No Hit
CTATATTACTGTGTGGGACGATCCGTGGGACCGTTTGAGTACTGGGGCCA12780.17757102841136455No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT12080.1678449157440754No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA12030.16715019341069762No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA12000.16673336001067093No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11500.15978613667689298No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11450.1590914143435152No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG11420.15867458094348852No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG11410.15853563647681296No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG11280.15672935841003066No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCCAACCATTCAGG11070.15381152460984393No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC10850.15075474634298164No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA10800.15006002400960386No Hit
CCCCTGAGGGCCGCTGATTAATAGTAGAGATGAGGAGTTTGGGGGCCATTCCTGGGAGTTGTTGGTACCAGTTTA10630.14769796807611932No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC10530.14630852340936376No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACTCCCGGGCAGAGGGTCAC10400.14450224534258146No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA10100.14033391134231468No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG9900.1375550220088035No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC9840.13672135520875017No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT9780.1358876884086968No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG9720.13505402160864347No Hit
TCCTCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACC9680.13449824374194122No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC9590.1332477435418612No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9550.13269196567515895No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC9540.1325530212084834No Hit
GTCTGAGGATGAGGCTATTTATTACTGTGCAGCATGGGATGACAGCCTGAATGGTTGGGTGTTCGGCGGAGGGAC9510.1321361878084567No Hit
CCCTCAGCGTCTGGGACTCCCGGGCAGAGGGTCACCTTCTCTTGTTCTGG9250.12852363167489217No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT9220.1281067982748655No Hit
GCATGGGATGACAGCCTGAATGGTTGGGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGG8900.12366057534124761No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC8700.12088168600773642No Hit
CTATTAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG8580.11921435240762972No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACAC8470.11768596327419857No Hit
ACCATATATTACGCAGACTCTGTGAAGGGCCGCTTCACCGTCTTCAGAGACAATGCCAACAACTCACTGTATCTG8450.11740807434084743No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT8430.11713018540749634No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA8340.11587968520741632No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT8180.11365657374060735No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTC8150.11323974034058068No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG8040.11171135120714953No Hit
CCTTGGTCCCTCCGCCGAACACCCAACCATTCAGGCTGTCATCCCATGCTGCACAGTAATAAATAGCCTCATCCT8030.11157240674047396No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG8010.11129451780712285No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC7980.11087768440709617No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC7970.11073873994042062No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGAAGCTATACCATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGG7900.1097661286736917No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTATTTATTACTGTGCAG7890.10962718420701614No Hit
GAGTTGGGGCTGTGCTGGGTTTTCCTTGTTGCTATTTTAGGAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGT7850.10907140634031391No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT7830.10879351740696278No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT7650.10629251700680273No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC7590.10545885020674936No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT7470.10379151660664265No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG7460.1036525721399671No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT7450.10351362767329153No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG7340.10198523853986038No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA7300.10142946067315814No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG7290.10129051620648259No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCCAACCATTCAGGCTGTCATCCCATGCTGCACAGTAAT7290.10129051620648259No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT208.495907E-569.0263439
ATCGCGT150.00236799268.9056737
GTCGTAT309.101874E-657.5219539
ATGTCGT309.196747E-657.42139437
AGGGGGG3000.054.55612670
GACGGAT200.00758985951.345358
TCCCTAG554.3505384E-449.59647470
GGTATCA5950.047.766331
GGAAACG1258.900315E-943.644970
GCCAGAG15400.042.9540970
GTCCTAG18650.041.31931770
GTATCAA16200.040.37191
GTGGTAT1450.040.1459471
GTCTTAT350.001282224939.8286349
TCGTATG3300.039.78145240
CGTATGC3500.039.52668441
GTATGCC3700.039.28994842
TGTCGTA451.004306E-438.31307638
CATGTCG451.01219644E-438.26220336
GCCGTCT3300.037.89494346
Produced by FastQC (version 0.11.5)