FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689168

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689168
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences719712
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTATTACTGTGCAGCATGGGATGACAGCCTGAATGGTTGGGTGTTCGG49550.6884698323773953No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39170.544245475968165No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACTC24270.33721822062158197No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG22860.3176270508203281No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC22370.3108187719532257No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT20540.28539193455159845No Hit
GTAATAAATAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG20530.28525299008492283No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCCAACCATTCAGGCTGTCATCCCATG18170.2524620959494909No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT16530.2296752034146992No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15890.22078275754746343No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA15510.2155028678137922No Hit
ATATTACTGTGTGGGACGATCCGTGGGACCGTTTGAGTACTGGGGCCAGG15250.21189031168022762No Hit
CAGTAATATAGAGCCGTGTCCTCGTCTCTCAGGTTGTTCATTTGCAGATA14830.20605464407985416No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT14680.20397047707972077No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACTCCCGGGCAGAGGGTCAC13830.19216019741229826No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG13800.19174336401227157No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG13470.1871581966119781No Hit
GATTAATAGTAGAGATGAGGAGTTTGGGGGCCATTCCTGGGAGTTGTTGG13360.185629807478547No Hit
GTAATATAGAGCCGTGTCCTCGTCTCTCAGGTTGTTCATTTGCAGATACA12900.17923836201147125No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC12860.178682584144769No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC12740.1770152505446623No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12550.17437530567782666No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTC12400.1722911386776933No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC12050.1674280823440487No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA11850.16464919301053754No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG11770.16353763727713308No Hit
CTATATTACTGTGTGGGACGATCCGTGGGACCGTTTGAGTACTGGGGCCA11520.1600640256102441No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG11150.15492308034324842No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG11130.1546451914098973No Hit
CTATTAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG11030.1532557467431417No Hit
CCCCTGAGGGCCGCTGATTAATAGTAGAGATGAGGAGTTTGGGGGCCATTCCTGGGAGTTGTTGGTACCAGTTTA10980.1525610244097639No Hit
GTCTGAGGATGAGGCTATTTATTACTGTGCAGCATGGGATGACAGCCTGAATGGTTGGGTGTTCGGCGGAGGGAC10740.1492263572095505No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT10730.14908741274287493No Hit
CCCTCAGCGTCTGGGACTCCCGGGCAGAGGGTCACCTTCTCTTGTTCTGG10710.1488095238095238No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG10540.1464474678760393No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA10110.14047285580899027No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA9900.1375550220088035No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT9880.13727713307545242No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT9660.1342203548085901No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC9490.1318582988751056No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT9300.12921835400826998No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA9290.12907940954159441No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA9280.12894046507491885No Hit
GCATGGGATGACAGCCTGAATGGTTGGGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGG9170.1274120759414877No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACAC9080.12616157574140768No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTAATTCTGTAAACTGGTACCAACAACTCCCAGGAATGGCCCC9050.125744742341381No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC8900.12366057534124761No Hit
GGGTCACCTTCTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTAATTCT8840.12282690854119427No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC8680.1206037970743853No Hit
ACCATATATTACGCAGACTCTGTGAAGGGCCGCTTCACCGTCTTCAGAGACAATGCCAACAACTCACTGTATCTG8580.11921435240762972No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA8580.11921435240762972No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC8480.11782490774087412No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT8300.11532390734071407No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGAAGCTATACCATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGG8070.11212818460717622No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC8060.11198924014050064No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCCAACCATTCAGG8030.11157240674047396No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT7980.11087768440709617No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC7730.1074040727402072No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG7690.10684829487350497No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG7690.10684829487350497No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT7660.10643146147347829No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA7570.10518096127339824No Hit
GAGTTGGGGCTGTGCTGGGTTTTCCTTGTTGCTATTTTAGGAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGT7330.10184629407318482No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC7330.10184629407318482No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC7320.10170734960650928No Hit
GTCTGGGACTCCCGGGCAGAGGGTCACCTTCTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTAATTCTGTAAA7290.10129051620648259No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCCAACCATTCAGGCTGTCATCCCATGCTGCACAGTAAT7270.10101262727313148No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)