FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689170

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689170
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1518167
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG91000.599407048104721No Hit
GTATTACTGTCAGCAGTCTGGTGGGTCACGGGGGACGTTCGGCCAAGGGA84640.5575144236437757No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT63040.4152375858518858No Hit
CAGTAATACACCGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACGCACTGCCA52760.3475243500879679No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC43040.2834997730816175No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAACCA42790.28185305042198916No Hit
GTGTATTACTGTCAGCAGTCTGGTGGGTCACGGGGGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAAACGAA41880.27585897994094194No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC39450.25985283568935436No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC37240.24529580737823967No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG35140.2314633370373615No Hit
CATCTATGGTGCATCCACCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGCGTCTGGGACAGACTTCA32320.2128883054367537No Hit
GTAATACACCGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACGCACTGCCA31820.20959486011749695No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT31320.20630141479824027No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTGGCAGCAGCTACTTAGCCTGGTACC29160.19207373101905126No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA28980.19088809070411883No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG28420.18719943194655134No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT28370.18687008741462569No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27970.18423533115922028No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27230.17936103208672036No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG26440.17415738848229478No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTAGCTGCTGCCAACACTCTGA25930.17079807425665294No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG25400.16730702221824081No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA25000.16467226596283546No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGGCAG24350.16039078704780171No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24180.15927101563925444No Hit
GAGTGAAGTCTGTCCCAGACGCACTGCCACTGAACCTGTCTGGGATGCCA24010.15815124423070717No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22380.1474146124899303No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21800.1435942159195925No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCGGTGTATTACTGTCAGCAGTCTGGTGGGTCA21730.14313313357489657No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21700.14293552685574118No Hit
GTCAGAGTGTTGGCAGCAGCTACTTAGCCTGGTACCAGCAGAAACCTGGC20720.13648037402999802No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCGGTGTATTACTGTCAGCAGT20300.13371387996182238No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC20170.13285758417881563No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT19990.1316719438638832No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAAC19650.12943240104678866No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19550.12877371198293733No Hit
GTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCCCTTGGCCGAACGTC18810.12389941291043739No Hit
GTCCCAGACGCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGGT18340.12080357431033609No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT18270.12034249196564015No Hit
GTAATACATGGCGGTGTCCGAGGCCTTCAGGCTACTCCACTGCAGGTAGG17850.11757599789746451No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17790.11718078445915371No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17480.11513884836121455No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17200.1132945189824308No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAACCACCGGAGAAATTGTGTTGACGCAGTCTC17050.11230648538665378No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16640.10960586022486327No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT16590.1092765156929376No Hit
GTCTGGTGGGTCACGGGGGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAAACGAACTGTGGCTGCACCAT16580.10921064678655248No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16410.10809087537800519No Hit
CTGCTACTCTGGCTCCCAGAAACCACCGGAGAAATTGTGTTGACGCAGTC16380.10789326865884978No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA16300.1073663174077687No Hit
GTTCAGTGGCAGTGCGTCTGGGACAGACTTCACTCTCACCATCAGCAGAC15850.10440221662043768No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15760.10380939646297145No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15440.10170159145864717No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG15420.10156985364587691No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC15290.10071355786287015No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTGTG33400.041.17442370
GGTATCA14550.038.6913721
GTATCAA38400.038.538421
GTGGTAT4650.038.4223181
AACGCAC552.1224788E-433.73838466
TGGTATC5200.033.0596922
TATCAAC45850.031.9802552
ATCAACG46200.031.4440633
TCAACGC46450.031.277924
AACGCAG46700.031.2548456
CAACGCA47450.030.6897835
ACGCAGA48750.030.0096867
CGCAGAG49150.029.9026228
AGAGTAC50400.029.28510511
TTAACGG953.4906407E-728.77049335
GCAGAGT51400.028.724829
TGTTAGA15750.028.01580270
GAACTGT30650.027.5789669
AATGGTA7000.027.31540570
CGCTAAG500.00866236426.9672287
Produced by FastQC (version 0.11.5)