FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689172

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689172
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences779792
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGTAGTTATCCGTGGACGTTCGGCCAAGGGGCCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT75490.968078667131748No Hit
CTACTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGACTGCAGGCTGCTGATGGTGAGAGTGTATTCTGCC69610.8926739438209162No Hit
GAGTATTACTGGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATAGGACGT52360.6714611075774053No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37760.4842316925539118No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36760.4714077600180561No Hit
GAATACACTCTCACCATCAGCAGCCTGCAGTCTGATGATTTTGCAACTTATTACTGCCAACAGTATAGTAGTTAT29160.37394587274555263No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24870.31893120216673165No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22850.2930268584443031No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22730.2914879865400004No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21500.27571454952089786No Hit
GAGTGTATTCTGCCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA20390.26147998440609804No Hit
GTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA18450.23660155528653792No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18410.23608859798510373No Hit
GATCTATAGGACGTCTAGTTTAGAAAATGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGGCAGAATACA18260.23416500810472538No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC16680.21390319469807334No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16550.2122360834684121No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16410.21044073291339227No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16230.20813242505693827No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA16070.20608059585120134No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTACTGGCTGGTTGGCCTGGTATCAGCAGAAA16030.20556763854976712No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15770.20223341609044462No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15710.2014639801382933No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15280.19594968914787536No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG15250.19556497117179963No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14410.18479286784168086No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14120.1810739274062827No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG13690.17555963641586472No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13580.1741490038369206No Hit
GTAGTTATCCGTGGACGTTCGGCCAAGGGGCCAAGGTGGAAATCAAACGA13420.17209717463118368No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13330.1709430207029567No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13250.16991710610008823No Hit
CAGTATAGTAGTTATCCGTGGACGTTCGGCCAAGGGGCCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT13010.16683936229148286No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGCCC12830.16453105443502883No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT12560.16106859265034779No Hit
GATAACTACTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGACTGCAGGCTGCTGATGGTGAGAGTGTATT12560.16106859265034779No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT12280.1574778915403082No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11930.1529895151527587No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11850.15196360054989022No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC11010.14119149721977142No Hit
GTATTACTGGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCT11000.14106325789441287No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGCCCCTTGGCCGAACGTC10880.1395243859901102No Hit
ACTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGACTGCAGGCTGC10530.13503600960256068No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10500.134651291626485No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10370.13298418039682378No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10350.13272770174610665No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10280.13183002646859676No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG10140.13003467591357695No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG10040.1287522826599914No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA10010.12836756468391572No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9750.12503334222459322No Hit
CTATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGC9480.12157088043991218No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT9470.12144264111455362No Hit
ACCTTGATGGGACCCCATTTTCTAAACTAGACGTCCTATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTG9440.12105792313847795No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9410.12067320516240228No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9360.1200320085356095No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTACTGG9220.11823665798058969No Hit
ACTTATTACTGCCAACAGTATAGTAGTTATCCGTGGACGTTCGGCCAAGGGGCCAAGGTGGAAATCAAACGAA8970.11503067484662577No Hit
GGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAGCAGCAGGAGCCCCAGGAG8810.11297884564088886No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC8810.11297884564088886No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA8700.11156821306194473No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC8540.10951638385620782No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC8370.10733631532511233No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA8360.10720807599975378No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT8250.10579744342080966No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA8110.10400209286578985No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA8030.1029761782629214No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC7910.10143730635861871No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC7890.10118082770790159No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT7840.1005396310811088No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGGCAGAATACACTCTCACCATCAGCAGCCTGCAGTCTGATGATTTTG7830.10041139175575024No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)