FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689173

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689173
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences408814
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATATGGCCGTGTCCTCGGCTCTCAGGCTGTTCATCTGCAGATACA12850.31432387344856094No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT11320.2768985406566311No Hit
ATATATTACTGTGCGAAAGATGATTGTCGTGGTGGTAGCTGCGACTATGAGGTTCTCGTGGATGCTTTTGATGT9290.22724270695230592No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC8800.21525681605815847No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT7060.1726946728830226No Hit
CTCTTATAGTGGTGCTAAGACATTCTATGTAGACTCCGTGAAGGGCCGGT6970.17049318271879144No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG6460.15801807178814814No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT6430.15728424173340444No Hit
CTATAAGAGATGCCTGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCG6420.1570396317151565No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA6070.14847828107647978No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC5980.1462767909122486No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT5980.1462767909122486No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA5840.1428522506567779No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG5690.13918310038305928No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG5670.1386938803465635No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC5240.12817564956190344No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG5210.12744181950715974No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT5160.1262187694159202No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT5150.1259741593976723No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC5010.12254961914220158No Hit
CAGTAATATATGGCCGTGTCCTCGGCTCTCAGGCTGTTCATCTGCAGATA4930.12059273899621833No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG4760.1164343686860039No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG4730.11570053863126017No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT4660.11398826850352484No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT4650.11374365848527693No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC4610.11276521841228529No Hit
CTCCTGTGCAGCCTCCGGATTCCCCTTTAGCAGATTTGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG4600.11252060839403738No Hit
GTCCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG4430.10836223808382296No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC4410.10787301804732714No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG4300.10518230784660017No Hit
ATATTACTGTGCGAAAGATGATTGTCGTGGTGGTAGCTGCGACTATGAGG4260.10420386777360853No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC4110.10053471749988993No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAGTA200.00615043354.14468457
TAGGCAA1051.5243131E-953.2489770
ACTAGTA200.007241634751.95460542
TCTGGAG5250.050.58651770
CGTATGC2600.039.86461641
TTAGGCA1051.6880222E-938.6811269
GCCGTCT2450.038.50551646
CTTGAAA2550.038.21977657
CTGCTAA2750.038.1214270
AGACCGA2500.037.71862825
TATACAC2700.037.35523
ACCGAGG2550.037.03349327
ATGCCGT2550.036.8316444
ACGAGAC2750.036.69845622
GATCTCG2050.036.62163536
CGTCTTC2500.036.52350648
CCGTCTT2600.036.37652647
GACCGAG2600.036.28124626
TTATACA2700.036.1100272
GAGACCG2650.035.56182524
Produced by FastQC (version 0.11.5)