FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689174

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689174
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences408814
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28020.6853972711306364No Hit
GTAATATATGGCCGTGTCCTCGGCTCTCAGGCTGTTCATCTGCAGATACA12060.2949996820069763No Hit
ATATATTACTGTGCGAAAGATGATTGTCGTGGTGGTAGCTGCGACTATGAGGTTCTCGTGGATGCTTTTGATGT9140.22357355667858733No Hit
CTCTTATAGTGGTGCTAAGACATTCTATGTAGACTCCGTGAAGGGCCGGT6830.16706864246332073No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC6820.16682403244507282No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT6430.15728424173340444No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC6240.1526366513866942No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT5880.1438306907297695No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA5670.1386938803465635No Hit
CTCCTGTGCAGCCTCCGGATTCCCCTTTAGCAGATTTGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG5640.13796005029181974No Hit
CTATAAGAGATGCCTGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCG5320.13013252970788672No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC5150.1259741593976723No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT5070.12401727925168904No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG4940.12083734901446623No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC4870.11912507888673088No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA4780.11692358872249972No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT4640.11349904846702902No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG4630.11325443844878111No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT4620.1130098284305332No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC4590.11227599837578948No Hit
ATATTACTGTGCGAAAGATGATTGTCGTGGTGGTAGCTGCGACTATGAGG4390.10738379801083134No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG4330.10591613790134388No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG4300.10518230784660017No Hit
GTCCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG4290.10493769782835226No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT4180.10224698762762527No Hit
CAGTAATATATGGCCGTGTCCTCGGCTCTCAGGCTGTTCATCTGCAGATA4180.10224698762762527No Hit
GTGCAAAGTGGATGCGACATAGATCAGGCGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGCCTAAATCAT4120.10077932751813784No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)