FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689175

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689175
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1490271
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTATTGGAGTTGAATAAAACACT66870.44871033523432985No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT61610.41341474134570155No Hit
CTCCTAAAGTGATCATTTACTGGGCATCTTCCCGGGAATTTGGGGTCCCT60160.403684967364996No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC58890.3951630273956884No Hit
ATATTATATTACCCCACTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT49710.3335634928143942No Hit
ATATTACCCCACTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA48810.3275243227574045No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC45960.308400284243604No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG43740.29350366476969625No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC43610.29263134020590886No Hit
GGGTAATATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT40970.27491644137207255No Hit
ATATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC38970.2614960634676512No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG37810.25371224428308675No Hit
GTTTATTACTGTCAGCAATATTATATTACCCCACTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA35230.23639995678638315No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC34530.23170282451983565No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG34330.2303607867293935No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT34210.22955556405512825No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA33810.22687148847424393No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT33780.22667018280567763No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG33640.22573075635236814No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT32760.21982579007442268No Hit
AACCAGGACAGCCTCCTAAAGTGATCATTTACTGGGCATCTTCCCGGGAA30950.2076803480709213No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA29920.2007688534501443No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG29300.19660853629977368No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT28370.1903680605742177No Hit
GTTTTATTCAACTCCAATAATAAGAACTACTTAGCTTGGTACCAGCAGAA26740.17943045258211426No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAAGTGCAAGTCCAGCCAGAGTGTTTTATT26610.17855812801832685No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG26450.17748449778597317No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAAGTGCAAGTCCA26440.17741739589645103No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT26160.17553854298983204No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATATTACC25860.17352548630416884No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC24570.16486934255581703No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24460.16413122177107387No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG24360.16346020287585278No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG23960.1607761272949685No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA23930.1605748216264022No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG23370.15681711581316418No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC22780.15285810433135985No Hit
CTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAAGTGATCA22470.15077794575617456No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT22070.14809387017529027No Hit
GTGTTGCAGACCCAGGTCCTCATTTCTCTGCTGCTCTGGATCTCTGGTGC21620.14507428514679543No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA21580.14480587758870703No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTATTGGAGTTG21440.14386645113539753No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC21070.14138368122307957No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT20870.14004164343263742No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCCTCATTTCTCTGCT20670.13869960564219527No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA20230.13574712250322257No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAAGTGCAAGTCCAGC20140.1351432054975236No Hit
GTCTCTGGGCGAGAGGGCCACCATCAAGTGCAAGTCCAGCCAGAGTGTTTTATTCAACTCCAATAATAAGAACTA20000.1342037790442141No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT19670.13198941668998457No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA19640.13178811102141824No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG19630.13172100913189613No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT19600.13151970346332983No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC19540.13111709212619718No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA19460.1305802770100203No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC19190.12876852599292343No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGTGGGGTAAT18990.1274264882024813No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC18680.12534632962729597No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA18580.1246753107320749No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATATTACCCCACTCACTTT18540.12440690317398649No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA18460.12387008805780962No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATATTACCCCAC18180.12199123515119062No Hit
GCTCTGGATCTCTGGTGCCTGCGGGGACATCGTGATGACCCAGTCTCCAG18170.12192413326166851No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT18140.12172282759310218No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT18080.12132021625596956No Hit
ACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAAGTGATCATT17990.12071629925027058No Hit
GGATCTCTGGTGCCTGCGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG17980.12064919736074849No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17470.11722700099512103No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC17260.11581786131515677No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC17200.11541524997802413No Hit
AGTCCAGCCAGAGTGTTTTATTCAACTCCAATAATAAGAACTACTTAGCTTGGTACCAGCAGAAACCAGGACAGC16840.11299958195522827No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC16830.11293248006570616No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC16780.11259697061809562No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC16730.11226146117048509No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA16480.11058391393243241No Hit
TTATTACTGTCAGCAATATTATATTACCCCACTCACTTTCGGCGGAGGGA16450.11038260826386609No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA16340.10964448747912292No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC16320.10951028370007872No Hit
GAATAAAACACTCTGGCTGGACTTGCACTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA16300.10937607992103449No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG16230.10890636669437974No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT16190.10863795913629132No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC16090.10796694024107026No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC16040.10763143079345971No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT15980.10722881945632706No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG15870.10649069867158391No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT15790.10595388355540704No Hit
GTGCAAGTCCAGCCAGAGTGTTTTATTCAACTCCAATAATAAGAACTACT15740.10561837410779651No Hit
GTAAATGATCACTTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTA15740.10561837410779651No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC15580.10454474387544278No Hit
TTTCTCTGCTGCTCTGGATCTCTGGTGCCTGCGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGT15560.10441054009639858No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG15040.100921241841249No Hit
GTCAGGGACCCCAAATTCCCGGGAAGATGCCCAGTAAATGATCACTTTAGGAGGCTGTCCTGGTTTCTGCTGGTA14960.10038442672507215No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCCA350.00439525658.94782370
AACGTAT200.007714925351.13621518
GGTATCA10450.047.9587141
TCGTATG1050.042.6803440
CGTATGC1200.040.26857441
CGGTTGC350.00134488839.44676241
AGGGTCG700.001369563339.2985570
ACGAGAC2250.037.8901222
TGGTATC2000.037.4910852
GTATCAA23950.035.7301371
ATCTCCG2550.034.75342610
GACAGGT3050.034.6520041
CGAGACT2600.034.10912323
GTGGTAT2300.034.09311
GTCGGTT652.104049E-433.7879569
AATGGTA3900.033.5045370
CACGAGA2650.033.45432721
ATGCCGT1253.6379788E-1233.2686744
TTACATA450.003862691831.8136663
TGCGTGA450.004191736731.28675755
Produced by FastQC (version 0.11.5)