FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689177

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689177
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1436962
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTATTACTGTGTGCTATATGTGGGTGGTGGCATATTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTACGT36240.2521987359443047No Hit
GTAATAGTCAGATTCATCATCTGTCTGGGCCCCCGTGATGCTGAGGGCAG34240.23828048340874705No Hit
ATATGTGGGTGGTGGCATATTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCAGCCCAAGGCTGCCCC31050.2160808706145326No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG29540.20557258995018657No Hit
CAGTAATAGTCAGATTCATCATCTGTCTGGGCCCCCGTGATGCTGAGGGC29300.20390239964591964No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT28940.20139711418951928No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA26890.18713090534057267No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC26790.1864349927137948No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG24500.17049859356058128No Hit
CCCACATATAGCACACAGTAATAGTCAGATTCATCATCTGTCTGGGCCCCCGTGATGCTGAGGGCAGCTTTGTTC24260.16882840325631435No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT23930.16653189158794734No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTC23770.16541843138510273No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC23760.16534884012242493No Hit
CACTTAGCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCT23320.16228682456460225No Hit
GTGGTGACCCAGGAGCCATCGATCTCAGTGTCCCCTGGAGGGACAGCCACATTCACTTGTGGCTTGGGTTCTGG23180.16131254688711322No Hit
CTATTACTGTGTGCTATATGTGGGTGGTGGCATATTGGTGTTCGGCGGAG22730.15818094006661276No Hit
CTCATCTACAGTACAAATATTCGCTCTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCCTTGGGAACAAAG22560.15699788860109037No Hit
GTGCTATATGTGGGTGGTGGCATATTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCAGCCCAAGG22450.1562323847116347No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG22360.1556060633475346No Hit
GTAGATGAGGGTGCGTGGAGCCTGGCCTGGGGTCTGCTGGTACCAGCTGG22210.15456219440736776No Hit
GGATGATGCTTCTCCTCGGACTCCTTGCTTTTGGATCAGGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCAT21790.15163936137490067No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT21590.1502475361213449No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT21190.14746388561423335No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG20490.14259249722678818No Hit
GCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCAATATGCCACCACCCACATATAGCACA19710.1371643787379207No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19620.1365380573738206No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA19610.1364684661111428No Hit
CTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCCTTGGGAACAAAGCTGCCCTCAGCATCACGGGGGCCCAGA19290.1342415457054536No Hit
CCCCAGGCCAGGCTCCACGCACCCTCATCTACAGTACAAATATTCGCTCT19200.1336152243413535No Hit
GTACAAATATTCGCTCTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATC19060.13264094666386445No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG19020.13236258161315329No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC18900.13152748646101986No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC18900.13152748646101986No Hit
CATATAGCACACAGTAATAGTCAGATTCATCATCTGTCTGGGCCCCCGTG18880.13138830393566428No Hit
CCCTCATCTACAGTACAAATATTCGCTCTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCCTTGGGAACAAAG18850.13117953014763092No Hit
GTCTCTAGTAGTCATAACCCCAGCTGGTACCAGCAGACCCCAGGCCAGGC18760.1305532087835308No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT18700.1301356612074641No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG18160.12637773302286354No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC17610.12255021357558515No Hit
GACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCAATATGCCACCACCCACATATAGCACACAGTAATAGT17180.11955778928044027No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA16980.11816596402688449No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGT16840.11719168634939546No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG16810.11698291256136209No Hit
GGCCCAGACAGATGATGAATCTGACTATTACTGTGTGCTATATGTGGGTG16670.11600863488387306No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC16580.11538231351977297No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA16460.11454721836763951No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC16320.11357294069015048No Hit
CCCCTGGAGGGACAGCCACATTCACTTGTGGCTTGGGTTCTGGCTCAGTC16100.11204193291123914No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC16010.11141561154713903No Hit
GTCTGGGCCCCCGTGATGCTGAGGGCAGCTTTGTTCCCAAGGATGGAGCC15590.10849277851467191No Hit
CCCCAGAAGAGCGAATATTTGTACTGTAGATGAGGGTGCGTGGAGCCTGG15550.10821441346396078No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA15520.10800563967592741No Hit
CTACTAGAGACTGAGCCAGAACCCAAGCCACAAGTGAATGTGGCTGTCCCTCCAGGGGACACTGAGATCGATGG15040.10466525906739357No Hit
CTCTAGTAGTCATAACCCCAGCTGGTACCAGCAGACCCCAGGCCAGGCTC14720.10243833866170435No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT14710.10236874739902656No Hit
ACTAGAGACTGAGCCAGAACCCAAGCCACAAGTGAATGTGGCTGTCCCTCCAGGGGACACTGAGATCGATGGCTC14520.10104651340814857No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG6100.047.6594970
AATCGCG1250.046.51628536
CGCGTAT851.8189894E-1244.48673239
GGTATCA19350.043.3696671
CGTATGC4500.040.6556241
TCGTATG4100.040.33220340
TCTCGTA4000.040.31494538
GTGGTAT5050.038.8644031
TGGTATC5250.038.658152
CGCTCAT7650.037.6813428
ATCTCGT4750.037.4922637
GAGACGC7900.037.2772324
ATCTCCG8100.037.16810
ATCGCGT1300.036.88217537
GACGCTC7900.036.8731526
ATGCCGG852.7921487E-936.76091844
ACGCTCA7850.036.69825727
AATCTCG5150.036.52763736
TTATACA8450.036.427872
GAATCGC1700.036.17691835
Produced by FastQC (version 0.11.5)