FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689178

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689178
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1436962
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG64510.44893323553441217No Hit
GACTATTACTGTGTGCTATATGTGGGTGGTGGCATATTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTACGT34840.24245595916941434No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG32590.226797925066912No Hit
ATATGTGGGTGGTGGCATATTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCAGCCCAAGGCTGCCCC30610.21301885505670992No Hit
GTAATAGTCAGATTCATCATCTGTCTGGGCCCCCGTGATGCTGAGGGCAG29940.20835624045729811No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT27850.19381166655764037No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG27580.19193270246534008No Hit
GTGGTGACCCAGGAGCCATCGATCTCAGTGTCCCCTGGAGGGACAGCCACATTCACTTGTGGCTTGGGTTCTGG27040.1881747742807395No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC26410.18379052473203886No Hit
CAGTAATAGTCAGATTCATCATCTGTCTGGGCCCCCGTGATGCTGAGGGC25320.17620507710015992No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA24400.16980268093380338No Hit
CACTTAGCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCT23610.16430497118225812No Hit
GTGCTATATGTGGGTGGTGGCATATTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCAGCCCAAGG23220.16159091193782438No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG23210.1615213206751466No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT22990.15999031289623525No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC22980.15992072163355747No Hit
CTCATCTACAGTACAAATATTCGCTCTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCCTTGGGAACAAAG22760.15838971385464612No Hit
GGATGATGCTTCTCCTCGGACTCCTTGCTTTTGGATCAGGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCAT21980.15296159536577864No Hit
CTATTACTGTGTGCTATATGTGGGTGGTGGCATATTGGTGTTCGGCGGAG21580.1501779448586671No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20470.1424533147014326No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTC20070.13966966419432109No Hit
GCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCAATATGCCACCACCCACATATAGCACA20060.13960007293164328No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG19650.13674683116185396No Hit
GTAGATGAGGGTGCGTGGAGCCTGGCCTGGGGTCTGCTGGTACCAGCTGG19550.1360509185350761No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT19330.13451991075616473No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT19220.13375440686670906No Hit
CTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCCTTGGGAACAAAGCTGCCCTCAGCATCACGGGGGCCCAGA19170.13340645055332012No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC18860.1312491214103087No Hit
GTCTCTAGTAGTCATAACCCCAGCTGGTACCAGCAGACCCCAGGCCAGGC18840.13110993888495312No Hit
CCCTCATCTACAGTACAAATATTCGCTCTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCCTTGGGAACAAAG18740.13041402625817525No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA18530.12895260974194167No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG18210.12672568933625247No Hit
CCCCAGGCCAGGCTCCACGCACCCTCATCTACAGTACAAATATTCGCTCT17830.1240812213544965No Hit
GTACAAATATTCGCTCTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATC17400.1210887970593516No Hit
GGCCCAGACAGATGATGAATCTGACTATTACTGTGTGCTATATGTGGGTG17110.11907065044169574No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG17040.11858351160295123No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA17000.11830514655224006No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC16500.11482558341835065No Hit
GACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCAATATGCCACCACCCACATATAGCACACAGTAATAGT16320.11357294069015048No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC16240.11301621058872817No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC15860.11037174260697222No Hit
CATATAGCACACAGTAATAGTCAGATTCATCATCTGTCTGGGCCCCCGTG15780.1098150125055499No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC15740.10953664745483875No Hit
CTCTAGTAGTCATAACCCCAGCTGGTACCAGCAGACCCCAGGCCAGGCTC15680.10911909987877202No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15630.10877114356538309No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG15410.10724013578647174No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT15080.10494362411810473No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA15050.10473485033007136No Hit
CCCCTGGAGGGACAGCCACATTCACTTGTGGCTTGGGTTCTGGCTCAGTC14980.10424771149132683No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT14840.1032734338138378No Hit
GCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACT14820.10313425128848222No Hit
GGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTTTGGATCAGGAGTGG14810.10306466002580443No Hit
CCCCAGAAGAGCGAATATTTGTACTGTAGATGAGGGTGCGTGGAGCCTGG14580.10146406098421532No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)