FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689179

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689179
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences358346
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC16570.46240225926897466No Hit
GCCTTGGGCTGACCGAGGACGGTCAGCTGGGTGCCTCCTCCGAACACAACATGATCACCACTACTATCCCACAC13720.38287018691432306No Hit
CCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACG11560.32259324786658705No Hit
GGATAGTAGTGGTGATCATGTTGTGTTCGGAGGAGGCACCCAGCTGACCGTCCTCGGTCAGCCCAAGGCTGCCCC9880.2757111841627924No Hit
GGATTACCTGTGGGGGAAACAACATTGGAGATAAAAGTGTGCACTGGTATCAGCAGAAGCCAGGCCAGGCCC9300.25952570978886325No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCGTAAGT8370.23357313880997696No Hit
CCACAGGGGCCTGGCCTGGCTTCTGCTGATACCAGTGCACACTTTTATCT7690.21459706540606005No Hit
CTTCATGCGTGACCCGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG7560.21096928666707598No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAGCTGGGTGCCTC7550.2106902267640772No Hit
CTACTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGGTTCGAC7530.21013210695807963No Hit
CCCCAGGACAGACGGCCAGGATTACCTGTGGGGGAAACAACATTGGAGAT7480.20873680744308573No Hit
GCCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAGGATTAC7450.20789962773408938No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCCGGCAGCTGTAGCTTCT7350.2051090287041016No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG6530.18222611665820185No Hit
GTGCTCCCTTCATGCGTGACCCGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG6490.18110987704620674No Hit
CTTTTATCTCCAATGTTGTTTCCCCCACAGGTAATCCTGGCCGTCTGTCCTGGGGCCACTGACACCGAGGGTGG6160.17190090024724708No Hit
CCCCTGTGGTGGTCGTCTATGATGACAGCGGCCGGCCCTCAGGGATCCCT6100.1702265408292544No Hit
GTTTGGTGGTCTCCACTCCCACCTTGACGGGGCTGCCATCTGCCTTCCAG6050.16883124131426053No Hit
GGGCTGGATGACTTGGGATGGGGGCAGAGACCCCTCCCCTGGGAACCTGC6030.16827312150826296No Hit
CCCTTCATGCGTGACCCGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG5860.16352910315728375No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGCCTCTGTGACCTCCTATGTGCTGACT5810.16213380364228985No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT5680.15850602490330576No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGCCTCTGTGA5560.15515730606732042No Hit
CTATTACTGTCAGGTGTGGGATAGTAGTGGTGATCATGTTGTGTTCGGAG5550.15487824616432164No Hit
GGCTCAGGTAGCTGCTGGCCGCATACTTGTTGTTGCTTTGTTTGGAGGGTTTGGTGGTCTCCACTCCCACCTTGA5490.15320388674632895No Hit
GGCCAGGCCCCTGTGGTGGTCGTCTATGATGACAGCGGCCGGCCCTCAGG5430.1515295273283363No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT5150.1437158500443705No Hit
ACACTGGTGTGTCTCGTAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC5140.14343679014137176No Hit
GGATATTTATTGAGAAGGGCTGGATGACTTGGGATGGGGGCAGAGACCCCTCCCCTGGGAACCTGCAGCTCCAGG5060.1412043109173815No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGC5060.1412043109173815No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCATACTT4920.13729747227539862No Hit
GGTGTGTCTCGTAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA4800.1339487534394133No Hit
CCTCTCTCACTGCACAGCCTCTGTGACCTCCTATGTGCTGACTCAGCCAC4730.13199533411842188No Hit
CGGCCACCCTGACCATCAGCAGCGTCGAACCCGGGGATGAGGCCGACTAT4730.13199533411842188No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGCCTCTGTGACCTCCTA4690.13087909450642676No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC4660.1300419147974304No Hit
GGTAGCTGCTGGCCGCATACTTGTTGTTGCTTTGTTTGGAGGGTTTGGTGGTCTCCACTCCCACCTTGACGGGG4650.12976285489443162No Hit
GGGTGGCCGTGTTGCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGGCCGCTGTCATCATAGA4620.1289256751854353No Hit
GTAATACACGGCCGTGTCCGCAGCGGTCACAGACGTCAGCCTCAGGGAGAACTGGTTCTTGGACGTGTCCACTGA4560.12725131576744264No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC4530.12641413605844629No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCGTAAGTGACTTCTACC4510.12585601625244874No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG4500.12557695634944996No Hit
GGTATCAGCAGAAGCCAGGCCAGGCCCCTGTGGTGGTCGTCTATGATGACAGCGGCCGGCCCTCAGGGATCC4460.12446071673745487No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT4380.12222823751346464No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGCCTCTGTGACCTCCTATGTGCTGACTCAGC4340.12111199790146954No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCACCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACGGCTC4310.1202748181924732No Hit
GCTGATACCAGTGCACACTTTTATCTCCAATGTTGTTTCCCCCACAGGTAATCCTGGCCGTCTGTCCTGGGGCCA4300.11999575828947441No Hit
CCCCTCCCCTGGGAACCTGCAGCTCCAGGCCCCTGTGGGTGGGGTGAGGG4250.11860045877448054No Hit
CCACCAAACCCTCCAAACAAAGCAACAACAAGTATGCGGCCAGCAGCTAC4190.11692609935648787No Hit
CTCACTGCACAGCCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGG4070.11357738052050254No Hit
GCCATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA3990.11134490129651232No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGCCTCTG3920.10939148197552087No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTACGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG3920.10939148197552087No Hit
GCTGATGGTCAGGGTGGCCGTGTTGCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGGCCG3840.10715900275153065No Hit
CCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAGGATTACCTGTGGGGGAAACAACATTGGAGATAAAAGTGTG3790.10576370323653675No Hit
GACCATCAGCAGCGTCGAACCCGGGGATGAGGCCGACTATTACTGTCAGG3770.1052055834305392No Hit
GTAGTGGTGATCATGTTGTGTTCGGAGGAGGCACCCAGCTGACCGTCCTC3770.1052055834305392No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTACGAGACACACCAGTGTGGCC3760.10492652352754042No Hit
CTCCAGGCCCCTGTGGGTGGGGTGAGGGTCGGGGGCCTAAGAGCATTCTG3740.10436840372154287No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGG3730.10408934381854408No Hit
CTGCACAGCCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGT3700.10325216410954775No Hit
GACTATTACTGTCAGGTGTGGGATAGTAGTGGTGATCATGTTGTGTTCGG3650.10185686459455387No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCCGGCAGCTGTAGCTTCTGT3640.10157780469155508No Hit
ATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGGTTCGACGCTGC3640.10157780469155508No Hit
GGGTAGAAGTCACTTACGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG3600.10046156507955999No Hit
GTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAG3600.10046156507955999No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG1450.058.12390570
AATGGTA1350.057.22693370
GTTTTGG200.00780607850.9765471
TATGACC200.007810386450.9694374
CGTATGC1300.047.73482541
ATCTCGT1450.044.98327337
TATACAC1600.044.5982553
ATGCCGT1400.044.5713144
TCGTATG1400.044.27504740
TTATACA1550.043.8446772
AATCTCG1500.043.42872236
GCAATCT1500.043.33719334
GGCAATC1550.041.89800633
CTTATAC1650.041.1931761
GTGGTAT603.2671232E-739.648431
AGGCAAT1650.039.31458732
CACGAGA1700.037.97722621
CCGTCTT1750.037.79943547
CGGGATA451.0938126E-437.75514217
GTATGCC1650.037.6946542
Produced by FastQC (version 0.11.5)