FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689180

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689180
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences358346
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC16360.4565420013060003No Hit
GCCTTGGGCTGACCGAGGACGGTCAGCTGGGTGCCTCCTCCGAACACAACATGATCACCACTACTATCCCACAC13480.3761727492423524No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12370.34519710000948806No Hit
CCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACG10690.2983150363056934No Hit
GGATAGTAGTGGTGATCATGTTGTGTTCGGAGGAGGCACCCAGCTGACCGTCCTCGGTCAGCCCAAGGCTGCCCC9960.2779436633867826No Hit
CTACTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGGTTCGAC9040.25227015231089506No Hit
GGATTACCTGTGGGGGAAACAACATTGGAGATAAAAGTGTGCACTGGTATCAGCAGAAGCCAGGCCAGGCCC8630.24082869628794518No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCGTAAGT7740.21599236492105398No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAGCTGGGTGCCTC7310.20399278909210652No Hit
GTGCTCCCTTCATGCGTGACCCGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG7180.2003650103531224No Hit
CCCCAGGACAGACGGCCAGGATTACCTGTGGGGGAAACAACATTGGAGAT6920.19310945287515419No Hit
CTTCATGCGTGACCCGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG6730.1878073147181774No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCCGGCAGCTGTAGCTTCT6580.18362141617319575No Hit
GCCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAGGATTAC6450.17999363743421162No Hit
GGCTCAGGTAGCTGCTGGCCGCATACTTGTTGTTGCTTTGTTTGGAGGGTTTGGTGGTCTCCACTCCCACCTTGA6280.1752496190832324No Hit
CCACAGGGGCCTGGCCTGGCTTCTGCTGATACCAGTGCACACTTTTATCT6270.17497055918023363No Hit
GGGTGGCCGTGTTGCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGGCCGCTGTCATCATAGA6230.17385431956823852No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGCCTCTGTGACCTCCTATGTGCTGACT5940.16576158238127398No Hit
GGGCTGGATGACTTGGGATGGGGGCAGAGACCCCTCCCCTGGGAACCTGC5830.16269192344828742No Hit
GTTTGGTGGTCTCCACTCCCACCTTGACGGGGCTGCCATCTGCCTTCCAG5810.16213380364228985No Hit
CTTTTATCTCCAATGTTGTTTCCCCCACAGGTAATCCTGGCCGTCTGTCCTGGGGCCACTGACACCGAGGGTGG5610.1565526055823143No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG5590.15599448577631675No Hit
CTATTACTGTCAGGTGTGGGATAGTAGTGGTGATCATGTTGTGTTCGGAG5550.15487824616432164No Hit
CCCCTGTGGTGGTCGTCTATGATGACAGCGGCCGGCCCTCAGGGATCCCT5450.15208764713433384No Hit
CCCTTCATGCGTGACCCGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG5410.15097140752233876No Hit
GGTGTGTCTCGTAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA5180.14455302975336684No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT5100.14232055052937664No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGC5020.14008807130538642No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC4980.1389718316933913No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGCCTCTGTGA4980.1389718316933913No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGG4880.13618123266340354No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGCCTCTGTGACCTCCTATGTGCTGACTCAGC4830.13478593314840964No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG4690.13087909450642676No Hit
ACACTGGTGTGTCTCGTAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC4660.1300419147974304No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCATACTT4650.12976285489443162No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC4630.12920473508843408No Hit
GGTAGCTGCTGGCCGCATACTTGTTGTTGCTTTGTTTGGAGGGTTTGGTGGTCTCCACTCCCACCTTGACGGGG4590.12808849547643897No Hit
GGATATTTATTGAGAAGGGCTGGATGACTTGGGATGGGGGCAGAGACCCCTCCCCTGGGAACCTGCAGCTCCAGG4420.12334447712545975No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT4400.1227863573194622No Hit
GGCCAGGCCCCTGTGGTGGTCGTCTATGATGACAGCGGCCGGCCCTCAGG4310.1202748181924732No Hit
CCCCTCCCCTGGGAACCTGCAGCTCCAGGCCCCTGTGGGTGGGGTGAGGG4280.11943763848347687No Hit
CCTCTCTCACTGCACAGCCTCTGTGACCTCCTATGTGCTGACTCAGCCAC4230.11804233896848297No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCACCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACGGCTC4160.11608891964749153No Hit
GCTGATACCAGTGCACACTTTTATCTCCAATGTTGTTTCCCCCACAGGTAATCCTGGCCGTCTGTCCTGGGGCCA4150.11580985974449275No Hit
GGTATCAGCAGAAGCCAGGCCAGGCCCCTGTGGTGGTCGTCTATGATGAC4140.11553079984149396No Hit
GTAATACACGGCCGTGTCCGCAGCGGTCACAGACGTCAGCCTCAGGGAGAACTGGTTCTTGGACGTGTCCACTGA4110.11469362013249763No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCGTAAGTGACTTCTACC4110.11469362013249763No Hit
CTGCACAGCCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGT4090.11413550032650008No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGCCTCTGTGACCTCCTA4040.11274020081150621No Hit
GGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGCCTCTGTGACCTC3970.11078678149051475No Hit
CGGCCACCCTGACCATCAGCAGCGTCGAACCCGGGGATGAGGCCGACTAT3900.10883336216952331No Hit
GTAATAGTCGGCCTCATCCCCGGGTTCGACGCTGCTGATGGTCAGGGTGG3810.10632182304253432No Hit
GGCCTGGCCTGGCTTCTGCTGATACCAGTGCACACTTTTATCTCCAATGTTGTTTCCCCCACAGGTAATCCTGGC3800.10604276313953553No Hit
CTCCAGGCCCCTGTGGGTGGGGTGAGGGTCGGGGGCCTAAGAGCATTCTG3690.10297310420654898No Hit
GGGTGAGGGTCGGGGGCCTAAGAGCATTCTGCAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC3660.10213592449755265No Hit
CTCACTGCACAGCCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGG3640.10157780469155508No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAGCTGGGTGCCTCCTCCGAACACAACATGATCAC3640.10157780469155508No Hit
GCTGATGGTCAGGGTGGCCGTGTTGCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGGCCG3630.10129874478855631No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)