FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689181

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689181
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1619554
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGAGCCTCACCTCCGTGGTACACTTTTGGCC71480.44135607704343294No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT58320.3600991384047707No Hit
GTAATAGACGGCCGTGTCCGCGGCGGTCACAAAACTCAGTCTCAGGGAGAACTGGTTCTTGGACGTGTCTATTGA51130.3157042000451976No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC46690.28828924506376447No Hit
CTCCTGCAGGGCCAGTCAGAATATTAAGAACAAGGACTTAGTCTGGTATC45820.28291739577686204No Hit
CAGTAATACACTGCAGAATCTGCAGGCTCCAGCCTGCTGATGGTGAGAATGAAGTCTGTCCCAGACCCACTGCCA45120.2785952181896991No Hit
GTGTATTACTGTCAGCAGTATGGAGCCTCACCTCCGTGGTACACTTTTGG41260.2547614960662009No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATCA38140.23549693310627495No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC37450.23123650091321438No Hit
GTATGGAGCCTCACCTCCGTGGTACACTTTTGGCCAGGGGACCAAACTGG36010.22234516416247932No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTT35360.21833171354582806No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTG34910.21555317081122333No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32780.20240140186742772No Hit
GTCTGGGACAGACTTCATTCTCACCATCAGCAGGCTGGAGCCTGCAGATT31900.19696780718642293No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG31350.19357181051079494No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCGCCCTCTCCTGCAGGGCCAGTCAGAATATTAAGAA31040.19165770329362283No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30630.18912614213542742No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG29700.183383820483911No Hit
GTCTCTGGTGACTCCATCAATAATTACTATTGGAATTGGATCCGGCAGCC28800.1778267350147016No Hit
GTAATACACTGCAGAATCTGCAGGCTCCAGCCTGCTGATGGTGAGAATGAAGTCTGTCCCAGACCCACTGCCA28550.1762831001621434No Hit
CTCCTGCACTGTCTCTGGTGACTCCATCAATAATTACTATTGGAATTGGATCCGGCAGCCCGCCGGGAAGGGA28150.17381328439805033No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27870.17208441336318517No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCGCCCTCTCCTGC27200.16794747195832926No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG27110.16739176341140832No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26350.16269911345963148No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT26210.1618346779421989No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA25950.1602292976955384No Hit
GTCTATTACTGTGCGAGAGATCCATTTTTGTCGTCCTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTC25310.15627759247298947No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCGCCCTCTCCTGCAGGGCCAGTCAGA24260.14979432609224513No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23810.14701578335764046No Hit
GCTCCATACTGCTGACAGTAATACACTGCAGAATCTGCAGGCTCCAGCCTGCTGATGGTGAGAATGAAGTCTGTC23550.14541040311097994No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23420.14460771298764968No Hit
CTTTTGGCCAGGGGACCAAACTGGAGATCAAAAGAACTGTGGCTGCACCA22760.14053251697689612No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22620.13966808145946352No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTTTGCTGATACCAGACTAAGTCCTTGTTCTTAATATTCTGA21810.13466670453717505No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21800.13460495914307272No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21350.131826416408468No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21030.12985056379719354No Hit
GACTTAGTCTGGTATCAGCAAAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCAGCAGGGCCA20980.1295418368266819No Hit
ATCCATAACAGTGGAAGTACCTACTACAACCCCTCCCTCAAGAGTCGAGT20900.1290478736738633No Hit
GGCCAAAAGTGTACCACGGAGGTGAGGCTCCATACTGCTGACAGTAATACACTGCAGAATCTGCAGGCTCCAGC20690.12775122039771444No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCTTTTGATCTCCAGTTTGGTCC20590.12713376645669117No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT19580.12089748165235614No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC19350.11947733758800262No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT18720.11558737775955602No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18530.1144142152716118No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18470.11404374290699786No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT18350.11330279817776993No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18350.11330279817776993No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18100.11175916332521177No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAT17730.10947458374342565No Hit
CTGCTACTCTGGCTCCCAGATATCACCGGAGAAATTGTGTTGACGCAGTC17420.10756047652625352No Hit
GACTTCATTCTCACCATCAGCAGGCTGGAGCCTGCAGATTCTGCAGTGTATTACTGTCAGCAGTATGGAGCCTCA17170.10601684167369535No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17080.10546113312677441No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC16790.10367051669780694No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16750.10342353512139763No Hit
GTTATGGATACGCCCAATCCACTCCAGTCCCTTCCCGGCGGGCTGCCGGA16610.10255909960396503No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16380.10113895553961152No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16280.10052150159858826No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3050.036.798751
TCGTATG4300.036.05734340
GTATCAA30700.035.7835241
CGAGACG5850.035.46552323
CGTATGC4600.035.25456241
CTTATAC6200.035.1080931
AGACGTA6000.034.59063725
CGTTATA400.002769764334.04883227
ACGAGAC6000.034.00571422
CATTCCG652.040414E-433.96532469
ACGTAGA6100.033.49065427
GACGTAG6100.033.47926326
GAGACGT6100.033.45754224
TATACAC6700.032.9783973
CACGAGA6100.032.8887421
CGTAGAG6150.032.67786828
TATCAAC34050.032.046372
ATGCCGT5000.031.89604644
ATCTCGT4000.031.7277137
TTATACA7000.031.0794182
Produced by FastQC (version 0.11.5)