FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689182

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689182
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1619554
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGAGCCTCACCTCCGTGGTACACTTTTGGCC67780.418510281225572No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT60020.3705958554021663No Hit
GTAATAGACGGCCGTGTCCGCGGCGGTCACAAAACTCAGTCTCAGGGAGAACTGGTTCTTGGACGTGTCTATTGA51630.31879146975031397No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG50640.3126786757341836No Hit
CAGTAATACACTGCAGAATCTGCAGGCTCCAGCCTGCTGATGGTGAGAATGAAGTCTGTCCCAGACCCACTGCCA43920.2711857708974199No Hit
CTCCTGCAGGGCCAGTCAGAATATTAAGAACAAGGACTTAGTCTGGTATC43640.2694568998625547No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC40810.25198295333159626No Hit
GTGTATTACTGTCAGCAGTATGGAGCCTCACCTCCGTGGTACACTTTTGG40730.2514889901787776No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC38030.2348177337711493No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATCA37350.23061904697219113No Hit
GTATGGAGCCTCACCTCCGTGGTACACTTTTGGCCAGGGGACCAAACTGG35880.22154247403914903No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33280.20548867157254405No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG32480.2005490400443579No Hit
GTCTGGGACAGACTTCATTCTCACCATCAGCAGGCTGGAGCCTGCAGATT32440.20030205846794857No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT32390.19999333149743698No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTT32100.19820271506846948No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTG31210.19270737499336238No Hit
CTCCTGCACTGTCTCTGGTGACTCCATCAATAATTACTATTGGAATTGGATCCGGCAGCCCGCCGGGAAGGGA30820.19029930462337163No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCGCCCTCTCCTGCAGGGCCAGTCAGAATATTAAGAA29430.18171669484314817No Hit
GTAATACACTGCAGAATCTGCAGGCTCCAGCCTGCTGATGGTGAGAATGAAGTCTGTCCCAGACCCACTGCCA29090.17961735144366905No Hit
GTCTCTGGTGACTCCATCAATAATTACTATTGGAATTGGATCCGGCAGCC28730.1773945172559853No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA26520.16374878515937105No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26220.16189642333630125No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCGCCCTCTCCTGC25930.16010580690733373No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCGCCCTCTCCTGCAGGGCCAGTCAGA25730.1588708990252872No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25600.15806820890195697No Hit
GTCTATTACTGTGCGAGAGATCCATTTTTGTCGTCCTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTC25090.15491919380273828No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT24650.1522023964622359No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG24300.15004130766865445No Hit
CTTTTGGCCAGGGGACCAAACTGGAGATCAAAAGAACTGTGGCTGCACCA23350.1441754952289334No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22640.13979157224766817No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22270.1375069926658821No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22120.13658081175434716No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22080.13633383017793788No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21710.1340492505961518No Hit
GCTCCATACTGCTGACAGTAATACACTGCAGAATCTGCAGGCTCCAGCCTGCTGATGGTGAGAATGAAGTCTGTC21480.13262910653179827No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21200.1309002354969331No Hit
GACTTAGTCTGGTATCAGCAAAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCAGCAGGGCCA21090.13022103616180752No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21040.12991230919129587No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20630.12738074803310045No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTTTGCTGATACCAGACTAAGTCCTTGTTCTTAATATTCTGA20360.12571362239233763No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCTTTTGATCTCCAGTTTGGTCC19830.12244111650491431No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC19340.11941559219390029No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC19180.11842766588826308No Hit
GGCCAAAAGTGTACCACGGAGGTGAGGCTCCATACTGCTGACAGTAATACACTGCAGAATCTGCAGGCTCCAGC18980.11719275800621654No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAT18420.11373501593648622No Hit
ATCCATAACAGTGGAAGTACCTACTACAACCCCTCCCTCAAGAGTCGAGT18390.11354977975417925No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT18110.11182090871931408No Hit
CTGCTACTCTGGCTCCCAGATATCACCGGAGAAATTGTGTTGACGCAGTC18030.11132694556649546No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT17880.11040076465496057No Hit
GACTTCATTCTCACCATCAGCAGGCTGGAGCCTGCAGATTCTGCAGTGTATTACTGTCAGCAGTATGGAGCCTCA17540.10830142125548145No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17360.10719000416163957No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17110.10564636930908138No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17070.10539938773267209No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16790.10367051669780694No Hit
GTGCAGCCACAGTTCTTTTGATCTCCAGTTTGGTCCCCTGGCCAAAAGTGTACCACGGAGGTGAGGCTCCATA16520.10200339105704409No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16220.10015102923397429No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCGA306.563983E-445.6363135
GTGGTAT3000.039.6073651
CTATTGA15900.036.65973769
GTATCAA30800.036.374111
CAAAAGA35850.033.8790270
AATGGTA6800.033.62111770
GAGTAAG851.3923818E-731.952161
AAAGTCG450.003930627431.70072759
TATCAAC35050.031.6680872
TCAGTCT16250.031.19448336
CGGTCAC16150.031.13632224
GTGTCTA16600.030.85095465
GCGGTCA16500.030.68841423
TCTATTG17400.030.5863368
GTAGTTT1652.6743492E-630.30994870
CGTGTCT16650.030.18336764
CTCAGTC16900.030.17667635
GGCGGTC16950.030.06859222
TCAACGC36700.030.0630514
ATCAACG36850.030.0290953
Produced by FastQC (version 0.11.5)