FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689185

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689185
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences804250
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT49210.6118744171588437No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC24200.3009014609884986No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACGGGGGTGTTGCTTGTATATGAGGTG22070.27441715884364315No Hit
GGAATATAGAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA19060.236990985390115No Hit
GATTATTACTGCACCTCATATACAAGCAACACCCCCGTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT18530.23040099471557351No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG18380.22853590301523158No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACGGGGGTGTTGCTTGTATATGAGGTGCAGTAATAAT17870.22219459123406898No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG17850.2219459123406901No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG17280.21485856387939073No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT17210.2139881877525645No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAAGTAGCAGTGACATT15130.18812558284115635No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG14810.18414672054709358No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC14510.1804165371464097No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT13380.16636617967050046No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC13350.1659931613304321No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA13120.16313335405657445No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG12780.15890581286913272No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC12550.1560460055952751No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC12500.1554243083618278No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCC12310.153061858874728No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG12230.1520671433012123No Hit
CTATATTCCTGTGCGAGCTCCCCACCAATCTGCTGCTATGCGCAGGGATT12060.14995337270749146No Hit
CTCCTGTGCTGCCTCTGGTTTCACCTTTAGTAGTTATTGGATGACCTGGTTCCGCCAGGCTCCAGGGAAGGGG11610.14435809760646565No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCACCTCATAT11510.14311470313957103No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG11460.14249300590612374No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG11140.13851414361206094No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT10830.1346596207646876No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG10730.13341622629779296No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC10670.1326701896176562No Hit
GTCGATCACCATCTCCTGCACTGGAAGTAGCAGTGACATTGGTGGTTATAACTATGTCTCCTGGTACCAACAACA10610.13192415293751944No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA10480.13030774013055643No Hit
CTCCTGCACTGGAAGTAGCAGTGACATTGGTGGTTATAACTATGTCTCCTGGTACCAACAACACCCAGGCAGAGT10440.12981038234379857No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT10260.12757227230338825No Hit
GTCCCCAAACTCATCGTTTATGAGGTCAGTAATCGGCCCTCAGGGGTTTCTGATCGCTTCTCTGGCTCCAAGT10000.12433944668946223No Hit
CCACGGGGGTGTTGCTTGTATATGAGGTGCAGTAATAATCAGCCTCGTCC9990.12421510724277277No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA9840.12235001554243084No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC9780.12160397886229406No Hit
GATCAGCACTGAGCACGGAGGACTCACCATGAAATTGGGGCTGAGCTGGG9630.11973888716195213No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG9580.11911718992850481No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT9550.11874417158843643No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG9440.11737643767485235No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT9400.1168790798880945No Hit
CCCCCGTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC9310.11576002486788933No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA9300.11563568542119987No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC8910.11078644700031086No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT8810.10954305253341623No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG8770.10904569474665837No Hit
GTTTGGGGACTCTGCCTGGGTGTTGTTGGTACCAGGAGACATAGTTATAA8760.10892135529996891No Hit
CCTCATATACAAGCAACACCCCCGTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTG8710.10829965806652161No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGAACCAGGTCATCCAATAACTAC8600.10693192415293752No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT8590.10680758470624806No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA8430.10481815355921666No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACGGGGGTGTTGC8380.10419645632576936No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGC8360.10394777743239043No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC8310.10332608019894311No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC8190.10183400683866958No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG6450.0100.2152870
GACGTGT150.002144092770.65378650
TGGTATC1550.052.019982
TTATGCC200.007469043651.5546239
TTTTCCG200.00817234650.39435613
GGATGGG6450.048.9688370
CTCTTAT3150.046.945591
GTAGTTT803.453069E-545.90827670
GTGGTAT1650.044.8117031
CTAACGC307.1919884E-444.7949833
TCGTATG11100.044.08345840
TCTCGTA9900.043.98138838
AAAGGGG7900.043.84257568
TATACAC11900.043.7598083
ATCTCGT9850.043.75663437
TGCCGTC11000.043.73875845
TATCTCG10000.043.70695536
CTTGAAA11300.043.68672657
CTTATAC11850.043.6772271
TTATACA11850.043.6609342
Produced by FastQC (version 0.11.5)