FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689186

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689186
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences804250
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG104441.2986011812247436No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC21470.2669567920422754No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACGGGGGTGTTGCTTGTATATGAGGTG21430.2664594342555176No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG20610.25626359962698164No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG18840.23425551756294685No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG18330.22791420578178426No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACGGGGGTGTTGCTTGTATATGAGGTGCAGTAATAAT17900.22256760957413738No Hit
GATTATTACTGCACCTCATATACAAGCAACACCCCCGTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT17840.22182157289400065No Hit
GGAATATAGAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA16690.20752253652471248No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT16140.20068386695679205No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC14230.17693503263910476No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAAGTAGCAGTGACATT14210.17668635374572583No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA14200.17656201429903637No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG13820.1718371153248368No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC13320.1656201429903637No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC13230.16450108797015853No Hit
CTCCTGTGCTGCCTCTGGTTTCACCTTTAGTAGTTATTGGATGACCTGGTTCCGCCAGGCTCCAGGGAAGGGG13100.16288467516319552No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT12630.15704072116879078No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC12140.15094808828100714No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11910.14808828100714952No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG11780.1464718682001865No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT11750.14609884986011812No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG11550.1436120609263289No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG11470.14261734535281317No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG11040.13727074914516632No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCACCTCATAT10930.13590301523158224No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG10840.13478396021137706No Hit
GTCGATCACCATCTCCTGCACTGGAAGTAGCAGTGACATTGGTGGTTATAACTATGTCTCCTGGTACCAACAACA10670.1326701896176562No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC10640.1322971712775878No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA10520.13080509791731426No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT10160.12632887783649363No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC10150.12620453838980417No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG9980.12409076779608331No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC9980.12409076779608331No Hit
CTCCTGCACTGGAAGTAGCAGTGACATTGGTGGTTATAACTATGTCTCCTGGTACCAACAACACCCAGGCAGAGT9840.12235001554243084No Hit
CCACGGGGGTGTTGCTTGTATATGAGGTGCAGTAATAATCAGCCTCGTCC9770.1214796394156046No Hit
CTATATTCCTGTGCGAGCTCCCCACCAATCTGCTGCTATGCGCAGGGATT9540.11861983214174697No Hit
GTCCCCAAACTCATCGTTTATGAGGTCAGTAATCGGCCCTCAGGGGTTTCTGATCGCTTCTCTGGCTCCAAGT9540.11861983214174697No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG9510.11824681380167858No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA9230.11476530929437365No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA8980.11165682312713708No Hit
GATCAGCACTGAGCACGGAGGACTCACCATGAAATTGGGGCTGAGCTGGG8820.1096673919801057No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT8760.10892135529996891No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC8560.10643456636617966No Hit
CCTCATATACAAGCAACACCCCCGTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTG8360.10394777743239043No Hit
GTTTGGGGACTCTGCCTGGGTGTTGTTGGTACCAGGAGACATAGTTATAA8350.10382343798570097No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGC8150.10133664905191173No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC8070.10034193347839603No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGTT200.007325799751.80771641
CTACTAG200.00819836850.3538368
GCTACTA200.00819836850.3538367
GTAGTTT901.0577332E-649.91820570
GGATGGG7350.046.8619970
GTGGTAT1850.043.5492631
TGGTATC1900.042.392682
GTATCAA13400.037.577491
GGTATCA5450.036.9569441
AACTGTA850.002338726335.2363870
TCCGAGA4100.034.6992470
AGGATGG6300.034.1736669
ACCGGTA4400.034.0351470
GCTCGGG2204.3837645E-1034.0351470
GACAGCC1350.032.325921
CCGAAAT552.9811426E-431.83889847
CAACCGA553.0988257E-431.62945744
TATCAAC16000.031.4633182
GGTAGTT1002.030758E-631.3970569
TTGGTAG753.2117558E-530.36605867
Produced by FastQC (version 0.11.5)