FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689189

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689189
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1438945
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCAAAAAGCAAATTGTTGTTGCCTGCAGATGAGCAG74160.5153775856617174No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAAGAGC69060.4799349523435572No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCAAAAAGCAAATTGTTGTTGCCTGCAGATGAGCAGCAGTAAAAAT61290.4259370580529485No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG52050.36172334592357597No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG51780.3598469712184969No Hit
CCTCTGAAGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT50670.35213298631983847No Hit
CTTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC48650.3380949237114692No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAAGAGCTTCAAGCCAACAAGG45730.3178022787528363No Hit
TCTCTGGGCTCCAGCCTGAGGATGAGGCTGATTTTTACTGCTGCTCATCT41070.2854174412503605No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTT40850.2838885433425183No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC39490.27443717445767557No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT37110.25789727890920083No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC36170.2513647151211478No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC34820.24198284159575242No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGC32700.2272498253929094No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCTTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG32510.22592941356340931No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC31860.22141221519933005No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT31820.2211342337615406No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG30930.21494914677072438No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG30690.21328125814398746No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA30470.21175236023614524No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAACA30320.21070992984443462No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCAAAAAGCAAATTGTTGTTGC29610.20577575932367118No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG29430.20452484285361844No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT28140.19555994148490735No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG27080.18819343338348582No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCTTCAGAGGA26420.1836067396599592No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC26360.18318976750327495No Hit
CTCTGTTCCCGCCCTCCTCTGAAGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC26120.18152187887653803No Hit
GGTTATAACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAACTCATAATTTATGAGGTCACTAAG26060.1811049067198538No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG26010.18075742992261692No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT25920.18013197168759057No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA25750.17895055057698522No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA25350.17617073619909032No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC25200.1751283058073797No Hit
GCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG24330.16908220953545827No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC23730.1649124879686159No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT22930.15935285921282605No Hit
CTCCCTGACCGTCTCTGGGCTCCAGCCTGAGGATGAGGCTGATTTTTACT22760.15817143810222073No Hit
CCCCTGAGGGCCGCTTAGTGACCTCATAAATTATGAGTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGA22670.15754597986719437No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT22530.15657304483493115No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC21640.15038795784411496No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC21640.15038795784411496No Hit
CCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCGTCTCTGGGCTCCAGC21430.14892855529572013No Hit
GCTTGGTCCCTCCGCCAAAAAGCAAATTGTTGTTGCCTGCAGATGAGCAG21280.14788612490400954No Hit
GTTCCCGCCCTCCTCTGAAGAGCTTCAAGCCAACAAGGCCACACTGGTGT21080.14649621771506208No Hit
GCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC20840.14482832908832513No Hit
GACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCT20820.14468933836943038No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA20530.14267397294545658No Hit
GTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATCAGGGAC19850.13794828850303523No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC19800.13760081170579835No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG19700.1369058581113246No Hit
CCCTGACCGTCTCTGGGCTCCAGCCTGAGGATGAGGCTGATTTTTACTGC19080.1325971458255875No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC18730.13016480824492943No Hit
GCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCA18570.12905288249377148No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT18550.12891389177487675No Hit
AGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCA18310.12724600314813977No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT18170.12627306811587655No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT18110.1258560959591923No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA18010.1251611423647186No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC17920.12453568412969224No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG17660.12272880478406054No Hit
GCCTGAGGATGAGGCTGATTTTTACTGCTGCTCATCTGCAGGCAACAACA17460.12133889759511308No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCTTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC17370.12071343936008673No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC17280.12008798112506038No Hit
CTCAGGACGTCACCACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTC17170.11932353217113928No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT17130.11904555073334978No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC17090.11876756929556029No Hit
GCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGG17080.11869807393611292No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG16800.11675220387158648No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG16790.1166827085121391No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAAGAGCTTCAAGCCAACAAGGCC16690.11598775491766537No Hit
TCCTCAGGCTGGAGCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATCAGGGACC16420.11411138021258632No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCTTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC16040.11147055655358613No Hit
GGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAAGAG15720.10924670505127021No Hit
GATTTTTACTGCTGCTCATCTGCAGGCAACAACAATTTGCTTTTTGGCGGAGGGACCAAGCTGACCGTCCTAGGT15360.10674487211116478No Hit
GCTCCAGCCTGAGGATGAGGCTGATTTTTACTGCTGCTCATCTGCAGGCA15230.10584143243834894No Hit
CCTGAGGGCCGCTTAGTGACCTCATAAATTATGAGTTTGGGGGCTTTGCC15180.10549395564111207No Hit
GTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTA14990.10417354381161198No Hit
CTATGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAACTCATAA14970.10403455309271722No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC14970.10403455309271722No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC14730.10236666446598029No Hit
GGCTGGAGCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC14690.10208868302819078No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT554.1836756E-1156.40489639
AGGGGGG4600.046.30446670
TATATCG306.6201825E-445.5570955
CGTATGC3050.045.30935341
AATCGCG851.8189894E-1244.4783836
GCGTATG704.4383341E-1044.35856240
TCGTATG2700.042.16801540
AATCTCG2950.041.9426336
CGAGACG4700.041.4462123
ATGCCGT3100.041.37618344
TCTCGTA2800.040.583738
GTATGCC3550.039.9472742
CGCTCAT4900.039.78494628
ATCTCGT3050.039.47646337
ATATCGC350.001413581539.048946
GACGCTC5000.038.9756926
ACGAGAC5100.038.86292322
GGTATCA12200.038.6649671
GTGGTAT3900.038.564391
GAGACGC5100.038.19552624
Produced by FastQC (version 0.11.5)