FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689190

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689190
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1438945
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCAAAAAGCAAATTGTTGTTGCCTGCAGATGAGCAG69300.48160284097029415No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAAGAGC63800.4433803932742391No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCAAAAAGCAAATTGTTGTTGCCTGCAGATGAGCAGCAGTAAAAAT58470.40633936668878934No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG56190.39049442473478835No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG53730.3733985663107346No Hit
CCTCTGAAGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT46620.3239873657436525No Hit
CTTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC45770.3180802601906258No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAAGAGCTTCAAGCCAACAAGG43140.29980298065596667No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42170.29306193078957155No Hit
TCTCTGGGCTCCAGCCTGAGGATGAGGCTGATTTTTACTGCTGCTCATCT41340.28729381595543957No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTT40260.2797883171351233No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC39680.27575758628717567No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC38510.26762662923183306No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG37460.26032961648985886No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT34830.24205233695519984No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC34300.23836908290448905No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGC33650.2338518845404098No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC32140.2233580852638565No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG32110.2231495991855144No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT31760.22071726160485633No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG29530.20521979644809218No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCTTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG29430.20452484285361844No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAACA29220.20306544030522364No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG28150.19562943684435474No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA27270.1895138452129859No Hit
CTCTGTTCCCGCCCTCCTCTGAAGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC27110.18840191946182794No Hit
GGTTATAACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAACTCATAATTTATGAGGTCACTAAG26360.18318976750327495No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCAAAAAGCAAATTGTTGTTGC25860.17971499953090633No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT25820.17943701809311682No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCTTCAGAGGA25150.17478082901014286No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT25050.1740858754156691No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA24870.1728349589456164No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG24860.17276546358616904No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC24280.1687347327382214No Hit
GCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG24150.16783129306540556No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC23710.16477349724972115No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT23040.16011730816674716No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC22970.15963084065061556No Hit
CTCCCTGACCGTCTCTGGGCTCCAGCCTGAGGATGAGGCTGATTTTTACT22790.15837992418056285No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA22370.1554611190837732No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA21540.14969300424964121No Hit
CCCCTGAGGGCCGCTTAGTGACCTCATAAATTATGAGTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGA21490.14934552745240437No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC20780.1444113569316409No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT20670.14364690797771978No Hit
GCTTGGTCCCTCCGCCAAAAAGCAAATTGTTGTTGCCTGCAGATGAGCAG20300.14107557967816697No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC20250.14072810288093013No Hit
GCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC20220.140519616802588No Hit
GACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCT20150.14003314928645638No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT20120.13982466320811426No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC19830.13780929778414047No Hit
CCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCGTCTCTGGGCTCCAGC19680.13676686739242988No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC19520.1356549416412719No Hit
CCCTGACCGTCTCTGGGCTCCAGCCTGAGGATGAGGCTGATTTTTACTGC19050.13238865974724537No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG18730.13016480824492943No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT18710.1300258175260347No Hit
GTTCCCGCCCTCCTCTGAAGAGCTTCAAGCCAACAAGGCCACACTGGTGT18660.12967834072879783No Hit
GTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATCAGGGAC18440.12814944282095564No Hit
GCCTGAGGATGAGGCTGATTTTTACTGCTGCTCATCTGCAGGCAACAACA18440.12814944282095564No Hit
AGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCA18240.12675953563200817No Hit
GCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCA17810.12377123517577114No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT17540.12189486047069206No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCTTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC17510.12168637439234994No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG17230.1197405043278235No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC17140.11911504609279716No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC17140.11911504609279716No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA16790.1166827085121391No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT16780.11661321315269173No Hit
CTCAGGACGTCACCACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTC16750.11640472707434961No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCAAAAAG16620.11550128740153377No Hit
GCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGG16020.11133156583469138No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC15930.11070610759966502No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG15870.11028913544298079No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCTTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC15580.10827377001900698No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC15450.10737033034619113No Hit
GGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAAGAG15300.10632789995448054No Hit
GATTTTTACTGCTGCTCATCTGCAGGCAACAACAATTTGCTTTTTGGCGGAGGGACCAAGCTGACCGTCCTAGGT15300.10632789995448054No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC15240.1059109277977963No Hit
GCTCCAGCCTGAGGATGAGGCTGATTTTTACTGCTGCTCATCTGCAGGCA15040.10452102060884885No Hit
CTATGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAACTCATAA14690.10208868302819078No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG14680.10201918766874342No Hit
GGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG14490.10069877583924333No Hit
GGCTGGAGCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC14400.10007331760421698No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTTTA150.002466690368.203221
ACGAAGT501.897996E-458.67527870
ACGGTCG200.00770839451.147089
TTGCGCA450.0092069348.8960670
GTGGTAT2700.045.468811
GGTATCA10600.044.3964351
AATGGTA6400.043.54805470
GTATCAA27850.036.734231
GTAGTTT1655.4296834E-835.56077270
TGGTATC3650.035.5018122
TATCAAC30900.032.4450652
ATCAACG30950.032.2802283
TCTCGCG3900.031.970570
TCAACGC31500.031.5001074
CAACGCA31700.031.1938025
AACGCAG32200.030.7094276
CCAAGGG4350.030.34927770
CCCTTAT450.004893304330.312541
ACGCAGA33600.029.531357
CGCAGAG34200.029.0132588
Produced by FastQC (version 0.11.5)