FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689193

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689193
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1771268
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC68320.3857123823159454No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT56610.31960155097929843No Hit
CCCCTGGAGAGTCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG51080.28838097905003646No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC51020.2880422386674405No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA44300.25010331581669176No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC42970.24259457066914775No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG42690.24101378221703323No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG40690.22972243613050086No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC38890.21956022465262176No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG38140.21532596987017208No Hit
GTAATGGATACAACTATTTAAATTGGTACCTGCAGAAGCCAGGGCGGTCTCCACAACTCCTGATCTTTTTGGGGT37820.21351935449632695No Hit
CTACAAACGCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACG37630.21244667661810637No Hit
GGTCTAGTCAGAGCCTCCTGCAGAGTAATGGATACAACTATTTAAATTGGTACCTGCAGAAGCCAGGGCGGTCTC36480.20595415261835023No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG33990.19189642674061744No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT33350.1882831959929271No Hit
CTCCAGGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAATGTCC32400.18291980660182422No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG31720.17908074893240322No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT31360.17704830663682739No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30960.17479003741952093No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT30340.1712897201326959No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTCAA29440.16620861439375634No Hit
GCGTTTGTAGAGGTTGCATGCAGTAATAAATCCCAACATCCTCAGCCTCAACTCTGCTGATTTTGAGTGTAAAAT28670.1618614461504414No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28590.16140979230698008No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT27840.15717553752453045No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGACATTGTGATGA27760.15672388368106915No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG27610.15587703272457923No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC27340.15435270100289736No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGACATTGTGATGACTCAGTCTCCACTCTCC27290.15407041735073407No Hit
GAGTTGAGGCTGAGGATGTTGGGATTTATTACTGCATGCAACCTCTACAAACGCCGCTCACTTTCGGCGGAGGGA27030.15260254235948484No Hit
GTCAGGGACCCCGGAGGGCCGATTGGACCCCAAAAAGATCAGGAGTTGTGGAGACCGCCCTGGCTTCTGCAGGTA26460.14938450872482312No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGACATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCC26430.14921513853352514No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26370.14887639815092918No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG26140.14757789335097796No Hit
GACTAGACCTGCAGGAGATGGAGGCCGACTCTCCAGGGGTGACGGGCAGG25960.14656167220319002No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT25400.14340009529896097No Hit
GAGTAATGGATACAACTATTTAAATTGGTACCTGCAGAAGCCAGGGCGGT24510.13837544629045406No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG23970.13532678284709035No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC23850.1346493020818984No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG23650.13352016747324516No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG23630.13340725401237982No Hit
GATTTTACACTCAAAATCAGCAGAGTTGAGGCTGAGGATGTTGGGATTTATTACTGCATGCAACCTCTACAAACG23440.13233457613415925No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG22770.12855197519517092No Hit
GTAATAAATCCCAACATCCTCAGCCTCAACTCTGCTGATTTTGAGTGTAAAATCTGTGCCTGATCCACTGCCA22750.12843906173430558No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA22710.12821323481257493No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA22620.12770512423868097No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT22400.12646307616916244No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC22250.1256162252126725No Hit
ATCCATTACTCTGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCC22200.1253339415605092No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA22090.12471291752574991No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21850.12335795599536603No Hit
AGCCAGGGCGGTCTCCACAACTCCTGATCTTTTTGGGGTCCAATCGGCCC21840.12330149926493338No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT21820.12318858580406805No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG21710.12256756176930877No Hit
CTCCACAACTCCTGATCTTTTTGGGGTCCAATCGGCCCTCCGGGGTCCCT21390.12076094639546359No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTCAAAATCAGCAGAG20930.11816393679556114No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT20850.11771228295209986No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA20620.11641377815214864No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGTCGGCCTCCATCTCCT20400.11517173008263007No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC20260.11438133585657281No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGACATTGTGATGACTCAGTCTCCACT20050.1131957445174869No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT19980.11280054740445827No Hit
GCAGAAGCCAGGGCGGTCTCCACAACTCCTGATCTTTTTGGGGTCCAATCGGCCCTCCGGGGTCCCTGACAGGTT19710.1112762156827764No Hit
GTTGTGGAGACCGCCCTGGCTTCTGCAGGTACCAATTTAAATAGTTGTATCCATTACTCTGCAGGAGGCTCTGA19330.10913085992633526No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT19110.1078888118568167No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG18970.10709841763075943No Hit
GAAATAGACAGCCGTGTCCTCGGCTCTCAGACTGTTCATTTGGAGATGCA18960.10704196090032678No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC18920.10681613397859613No Hit
GGATACAACTATTTAAATTGGTACCTGCAGAAGCCAGGGCGGTCTCCACAACTCCTGATCTTTTTGGGGTCCAAT18810.10619510994383684No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT18470.10427558110912634No Hit
GCCTCAACTCTGCTGATTTTGAGTGTAAAATCTGTGCCTGATCCACTGCC18420.10399329745696305No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG18380.10376747053523237No Hit
GGCTTCTGCAGGTACCAATTTAAATAGTTGTATCCATTACTCTGCAGGAG18360.10365455707436706No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT18300.10331581669177109No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT18220.1028641628483098No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG18160.10252542246571383No Hit
CCACAACTCCTGATCTTTTTGGGGTCCAATCGGCCCTCCGGGGTCCCTGA17890.10100109074403195No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT17890.10100109074403195No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17840.10071880709186865No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA17810.10054943690057067No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCGT306.5877126E-445.6031337
TGGTATC4200.044.2565042
GTGGTAT4200.042.6604231
GGTATCA13900.042.5619851
CGTATGC6150.040.33329441
ATGCCGT5650.039.88219544
ACGCGTA6250.039.55845627
AGACGCG6250.038.98788525
GCGTAGT6300.038.75514229
GACGCGT6300.038.69376826
CGCGTAG6400.038.65207728
TCGTATG6250.038.50573740
GTAGTAA6650.037.80916631
AATCTCG5250.037.73801836
GTATGCC6600.037.66892642
GCCGTCT5850.037.4909246
CGAGACG6600.037.40880223
ACGAGAC6750.037.0701822
CGTAGTA6800.036.9332130
GTAATCT5500.036.55715634
Produced by FastQC (version 0.11.5)