FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689194

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689194
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1771268
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG67460.3808571034987365No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC58590.3307799836049655No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT57970.3272796663181404No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC52410.29588972419758053No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC46020.25981387345110957No Hit
CCCCTGGAGAGTCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG40490.22859330152184762No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA40290.22746416691319438No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC39390.2223830611742548No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG37740.21306770065286562No Hit
GGTCTAGTCAGAGCCTCCTGCAGAGTAATGGATACAACTATTTAAATTGGTACCTGCAGAAGCCAGGGCGGTCTC37730.21301124392243295No Hit
GTAATGGATACAACTATTTAAATTGGTACCTGCAGAAGCCAGGGCGGTCTCCACAACTCCTGATCTTTTTGGGGT36630.20680100357484016No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG34500.1947757199926832No Hit
CTACAAACGCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACG33630.18986398444504166No Hit
CTCCAGGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAATGTCC33030.18647658061908193No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT32590.1839924844800448No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG32110.18128256141927704No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT32000.18066153738451776No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT31860.1798711431584605No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT31530.17800807105418265No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG31210.1762014556803375No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT30230.1706686960979366No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG29190.16479719613293978No Hit
GCGTTTGTAGAGGTTGCATGCAGTAATAAATCCCAACATCCTCAGCCTCAACTCTGCTGATTTTGAGTGTAAAAT29080.1641761720981805No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTCAA27150.15328002312467678No Hit
GAGTTGAGGCTGAGGATGTTGGGATTTATTACTGCATGCAACCTCTACAAACGCCGCTCACTTTCGGCGGAGGGA26930.15203797505515823No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGACATTGTGATGACTCAGTCTCCACTCTCC26480.14949742218568846No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT26480.14949742218568846No Hit
GTCAGGGACCCCGGAGGGCCGATTGGACCCCAAAAAGATCAGGAGTTGTGGAGACCGCCCTGGCTTCTGCAGGTA26360.1488199414204965No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGACATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCC26040.14701332604665135No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC25510.14402111933372025No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGACATTGTGATGA25350.14311781164679768No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25190.1422145039598751No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA25090.14164993665554845No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG24520.13843190302088673No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24420.1378673357165601No Hit
GAGTAATGGATACAACTATTTAAATTGGTACCTGCAGAAGCCAGGGCGGT24310.13724631168180085No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG24260.13696402802963753No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23490.13261685978632257No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG23060.1301892203777181No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22980.1297375665342568No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG22780.12860843192560356No Hit
GATTTTACACTCAAAATCAGCAGAGTTGAGGCTGAGGATGTTGGGATTTATTACTGCATGCAACCTCTACAAACG22770.12855197519517092No Hit
ATCCATTACTCTGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCC22630.12776158096911366No Hit
GACTAGACCTGCAGGAGATGGAGGCCGACTCTCCAGGGGTGACGGGCAGG22430.12663244636046042No Hit
GTAATAAATCCCAACATCCTCAGCCTCAACTCTGCTGATTTTGAGTGTAAAATCTGTGCCTGATCCACTGCCA22360.1262372492474318No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC22330.1260678790561338No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA22200.1253339415605092No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA22090.12471291752574991No Hit
CTCCACAACTCCTGATCTTTTTGGGGTCCAATCGGCCCTCCGGGGTCCCT20880.11788165314339782No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTCAAAATCAGCAGAG20740.11709125891734057No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG20400.11517173008263007No Hit
GTTGTGGAGACCGCCCTGGCTTCTGCAGGTACCAATTTAAATAGTTGTATCCATTACTCTGCAGGAGGCTCTGA20340.1148329897000341No Hit
AGCCAGGGCGGTCTCCACAACTCCTGATCTTTTTGGGGTCCAATCGGCCC20100.11347802816965022No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT19950.11263117721316028No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19850.11206660990883367No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA19760.1115584993349397No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC19750.11150204260450705No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT19680.11110684549147842No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC19480.1099777108828252No Hit
GGGTCTCTGGATCCAGTGGGGACATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGTCGG19460.10986479742195986No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT19450.1098083406915272No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT19440.10975188396109455No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGACATTGTGATGACTCAGTCTCCACT19360.10930023011763323No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA18670.10540471571777957No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT18610.1050659753351836No Hit
GCAGAAGCCAGGGCGGTCTCCACAACTCCTGATCTTTTTGGGGTCCAATCGGCCCTCCGGGGTCCCTGACAGGTT18590.10495306187431828No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG18500.10444495130042433No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT18500.10444495130042433No Hit
GGATACAACTATTTAAATTGGTACCTGCAGAAGCCAGGGCGGTCTCCACAACTCCTGATCTTTTTGGGGTCCAAT18320.10342873015263643No Hit
GAAATAGACAGCCGTGTCCTCGGCTCTCAGACTGTTCATTTGGAGATGCA18140.1024125090048485No Hit
GGCTTCTGCAGGTACCAATTTAAATAGTTGTATCCATTACTCTGCAGGAG18090.1021302253526852No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA18060.10196085516138721No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA17870.10088817728316664No Hit
GCCTCAACTCTGCTGATTTTGAGTGTAAAATCTGTGCCTGATCCACTGCC17730.10009778305710938No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)