FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689195

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689195
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1042346
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTATACAGCTCCACCAATAGGAACTACTTAGCTTGGTACCAGCAGAA66830.6411498677022792No Hit
CTATTATGCTACTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT57440.5510646176989215No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT46190.44313500507509024No Hit
GCATAATAGTGTTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC38290.3673444326547998No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36170.34700569676479787No Hit
ATGCTACTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA34840.33424601811682497No Hit
GAGTAGCATAATAGTGTTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT33290.3193757159330971No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG31190.2992288549099819No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29680.2847423024600277No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAAGGAGTCTGGAGA26100.25039670128728847No Hit
CTCCTTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAGCTCCAC25870.24819014031809017No Hit
GTTTATTACTGTCAACACTATTATGCTACTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA25810.24761451571742973No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25570.24531201731478797No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24750.23744514777242873No Hit
CTCCTAAGATGCTCATTTACTGGGCATCTACCCGGGACTCCGGGGTCCCT24080.23101733973172056No Hit
ACCCGGGACTCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGC23880.22909859106285246No Hit
TCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCCTATTGGTGGAGCTGTATAAAACACT23690.22727577982742778No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23420.2246854691244558No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22440.21528360064700208No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21480.20607360703643512No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21030.20175642253148188No Hit
AACCAGGACAGCCTCCTAAGATGCTCATTTACTGGGCATCTACCCGGGAC21030.20175642253148188No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20670.1983026749275193No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20210.1938895529891226No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19980.19168299201992428No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC19600.18803736954907488No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19210.18429580964478207No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC18990.18218518610902712No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG18630.17873143850506454No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA18310.17566144063487554No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAACACTATTATGCTACT17490.1677945710925163No Hit
GTCTCCAGACTCCTTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATA16870.16184645021902516No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC16800.16117488818492134No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16800.16117488818492134No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC16540.15868051491539278No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA16300.15637801651275104No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16050.1539795806766659No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15730.15090958280647693No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15220.14601677370086324No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC15140.14524927423331602No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14940.1433305255644479No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14940.1433305255644479No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14770.14169958919591No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14740.14141177689557977No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14720.14121990202869297No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAGCTCCACCAATAGGAACTA14640.14045240256114572No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14610.1401645902608155No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT14440.13853365389227762No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCTTGGCTGTGTCTCTGGGCGAGAGGG14260.13680678009029631No Hit
GTCCCGGGTAGATGCCCAGTAAATGAGCATCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTT14170.13594334318930568No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14150.13575146832241886No Hit
CTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGATGCTCATTTACTGGGCATCTACCCGGGACTC14080.135079906288315No Hit
GTCAGGGACCCCGGAGTCCCGGGTAGATGCCCAGTAAATGAGCATCTTAGGAGGCTGTCCTGGTTTCTGCTGGTA13850.13287334531911668No Hit
ACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGATGCTCATT13790.13229772071845627No Hit
CCTTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC13740.13181803355123922No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC13560.13009115974925792No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13490.12941959771515407No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13260.12721303674595577No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG12780.12260803994067228No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA12590.12078522870524759No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCCTATTGGTGGAGCTG12400.11896241746982288No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAACACTATTATGCTACTCCGT12380.11877054260293607No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12260.11761929340161521No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12150.11656398163373774No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12000.11512492013208667No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA11980.11493304526519985No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC11950.11464523296486961No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAACACTATTATGCTACTCCGTGGACGTT11900.11416554579765259No Hit
GTAATACACGGCTGTGTCCTCGGTTTTCAGGCTATTCATTTGCAGATACA11850.11368585863043557No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11650.11176710996156747No Hit
GCCTCCTAAGATGCTCATTTACTGGGCATCTACCCGGGACTCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGT11530.1106158607602466No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11500.11032804845991638No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC11350.10888898695826531No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT11330.10869711209137847No Hit
GTAAATGAGCATCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTA11210.10754586289005762No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT11180.1072580505897274No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11170.107162113156284No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC11130.10677836342251038No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11130.10677836342251038No Hit
CCCTTAGACTCTCCTGTGCAGCCTCTGGATTCGCTTTCAGTAACGCCTGG11090.10639461368873676No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA11050.10601086395496313No Hit
CATTTACTGGGCATCTACCCGGGACTCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCA10940.10495555218708566No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCTTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG10840.10399617785265161No Hit
GAACTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGATGCTCATTTACTGGGCATCTACCCGGGA10750.10313274095166097No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC10530.10102211741590604No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG10480.10054243024868902No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT10480.10054243024868902No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCTCG100.008142674138.4376570
CGCGGAT252.5821634E-455.1345139
ATCGCGT252.603165E-455.04417837
GTGGTAT1300.052.7137221
CTTATGG400.00726521851.9141270
GCGTATG200.00736667651.7360540
TGGTATC1450.047.2401732
GTATGGC306.2745414E-446.05434842
CTAACCA306.4683746E-445.77089732
GGTATCA5150.044.576361
CGTATGC404.986832E-543.14672541
CATCGCG651.1719749E-842.3212836
CACGAGA1500.041.11867521
CGAGACT1450.040.17727723
ACGAGAC1450.040.1734222
CTTATAC1550.039.7903561
GCATCGC702.2631866E-839.27941535
AATGGTA2500.038.76254370
TATACAC1600.038.5302663
GACAGGT1800.038.0710221
Produced by FastQC (version 0.11.5)