FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689197

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689197
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1643524
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTATACAGGTCCAACAGTCAGAACTACATGAGCTACTTAAGTTGGTA101510.6176362499117749No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT69630.4236628123471272No Hit
CTCCTAAGCTGCTCATTTACTGGGCGTCTACCCGGGCATCCGGGGTCCCT64900.39488319002338873No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC61220.37249227878631525No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGCTGATACCAACTTAAGTAGCTCATG54640.332456355976548No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGTCAGGGAATCTGGAGA52730.32083498628556684No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC46670.28396299658538604No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC46270.28152920188570413No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG43530.2648577081928831No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG39000.23729498321898554No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT36680.22317897396083053No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT34910.2124094324147381No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC34560.21027986205251642No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG33440.2034652368934071No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG32500.1977458193491546No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA31420.1911745736600135No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT30550.18588107018820535No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT29480.17937066936655624No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC28490.17334702748484354No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC28430.17298195827989124No Hit
AACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCGTCTACCCGGGCA27490.1672625407356388No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG27360.1664715574582422No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTTGGCCGAACGTC27130.1650721255059251No Hit
ACTTAAGTTGGTATCAGCAGAAACCAGGACAGCCTCCTAAGCTGCTCATT26820.1631859346136716No Hit
GTCCCTGACCGATTCCGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC26070.15862256955176804No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATGTGGCAGTTTATTATTGTCAGCAATACTTTCATGTT26010.15825750034681577No Hit
GAACTACATGAGCTACTTAAGTTGGTATCAGCAGAAACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCGT25940.1578315862743714No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT25440.15478934289976903No Hit
GGTCCAACAGTCAGAACTACATGAGCTACTTAAGTTGGTATCAGCAGAAA24430.14864401128307222No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC24300.1478530280056756No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG23790.14474993976358116No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23780.14468909489608914No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA23360.14213361046142314No Hit
TTCATGTTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAGATCAAACGA23350.1420727655939311No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG23180.14103840284656627No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG23120.140673333641614No Hit
GCCTGAAGATGTGGCAGTTTATTATTGTCAGCAATACTTTCATGTTCCGT22590.13744855566453548No Hit
CATCATGATGACGCAGTCTCCAGATTCCCTGACTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAG22550.1372051761945673No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG22430.13647503778466272No Hit
GTCTCCAGATTCCCTGACTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATA22370.13610996857971042No Hit
GAACATGAAAGTATTGCTGACAATAATAAACTGCCACATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAAT22240.13531898530231382No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT22200.13507560583234562No Hit
GCTCATTTACTGGGCGTCTACCCGGGCATCCGGGGTCCCTGACCGATTCCGTGGCAGCGGGTCTGGGACAGATTT22070.134284622554949No Hit
GGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC21960.13361532901253648No Hit
GTCAGGGACCCCGGATGCCCGGGTAGACGCCCAGTAAATGAGCAGCTTAGGAGGCTGTCCTGGTTTCTGCTGATA20900.12716577305837942No Hit
CCTTGGTCCCTTGGCCGAACGTCCACGGAACATGAAAGTATTGCTGACAATAATAAACTGCCACATCTTCAGG20620.12546211676860208No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA20450.12442775402123729No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC19750.12016861329679396No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG19690.11980354409184168No Hit
GTTTATTATTGTCAGCAATACTTTCATGTTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAGATCAAACGAA19630.1194384748868894No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA19520.11876918134447687No Hit
GTAAATGAGCAGCTTAGGAGGCTGTCCTGGTTTCTGCTGATACCAACTTA19300.11743059425965183No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA19300.11743059425965183No Hit
GTCCTGGTTTCTGCTGATACCAACTTAAGTAGCTCATGTAGTTCTGACTGTTGGACCTGTATAAAACACTCTGG19260.11718721478968364No Hit
GACCGATTCCGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAG19090.11615285204231883No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT19010.11566609310238243No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC18750.1140841265475892No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT18710.11384074707762103No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT18540.1128063843302562No Hit
CACCATCAGCAGCCTGCAGCCTGAAGATGTGGCAGTTTATTATTGTCAGCAATACTTTCATGTTCCGTGGACGTT18390.1118937113178755No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC18320.11146779724543117No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT18160.1104942793655584No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA17920.10903400254574927No Hit
GGATCTCTGGTGCCTACGGGGACATCATGATGACGCAGTCTCCAGATTCCCTGACTGTGTCTCTGGGCGAGAGGG17750.10799963979838444No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAGGTCCAACAGTCAGAACTA17740.1079387949308924No Hit
GGTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGAT17580.10696527705101963No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT17550.1067827424485435No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17280.10513993102625821No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC17280.10513993102625821No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG17250.10495739642378207No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT17220.10477486182130594No Hit
TTCCCTGACTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAGGTCCAA17160.10440979261635364No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT17110.1041055682788934No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT17060.10380134394143317No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG16780.10209768765165583No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG16700.10161092871171945No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC16660.10136754924175127No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA16600.10100248003679897No Hit
CATTTACTGGGCGTCTACCCGGGCATCCGGGGTCCCTGACCGATTCCGTGGCAGCGGGTCTGGGACAGATTTCA16440.10002896215692621No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT204.82955E-4102.83111670
GTACGTA401.2212473E-554.74113569
TGCGTTA250.006925313752.55148769
ATCGTAT306.383078E-445.89591639
GTGGTAT3350.044.5876731
TCGTATG4650.042.28204340
CGTATGC4750.042.18981641
CTTATAC5400.042.1199651
TGGTACG456.4209176E-541.33846367
ATATCGT350.001383247439.22196637
AATCGCG350.001390794139.17857436
ATGCCGT4800.039.06094444
GTATGCC5150.038.98296442
CGTAATC350.001456920438.81005521
TATACAC6050.038.708663
GCCGTCT4550.038.2643346
ACGAGAC6000.037.92643422
ATCTCCG6150.037.52737810
TCTCCGA6150.037.52737811
CCTCCTA45150.037.5035670
Produced by FastQC (version 0.11.5)