FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689198

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689198
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1643524
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTATACAGGTCCAACAGTCAGAACTACATGAGCTACTTAAGTTGGTA91770.5583733489745206No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT69130.4206205689725249No Hit
CTCCTAAGCTGCTCATTTACTGGGCGTCTACCCGGGCATCCGGGGTCCCT59390.36135766803527053No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG57500.34985798807927354No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGCTGATACCAACTTAAGTAGCTCATG52310.31827950185090087No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC50570.30769249490728456No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC46320.2818334262231644No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC44390.27009036679719917No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGTCAGGGAATCTGGAGA39710.24161496881092093No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG39630.24112820987098452No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT37450.2278640287577182No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC35830.21800716022400646No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT32250.19622469766185344No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT32130.1954945592519489No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG31710.19293907481728287No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA30180.18362981009099957No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG29940.18216953327119043No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG29140.17730194387182663No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT28250.17188675066503442No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTTGGCCGAACGTC28090.17091323278516166No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC27830.1693312662303684No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC27220.1656197293133535No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26800.1630642448786875No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA26120.15892679388922826No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATGTGGCAGTTTATTATTGTCAGCAATACTTTCATGTT25690.15631046458707024No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25480.15503272236973722No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG25250.15363329041742013No Hit
GTCCCAGACCCGCTGCCACGGAATCGGTCAGGGACCCCGGATGCCCGGGT24360.1482180972106279No Hit
AACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCGTCTACCCGGGCA24130.1468186652583108No Hit
GTCCCTGACCGATTCCGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC22910.139395591424281No Hit
ACTTAAGTTGGTATCAGCAGAAACCAGGACAGCCTCCTAAGCTGCTCATT22790.13866545301437644No Hit
GCCTGAAGATGTGGCAGTTTATTATTGTCAGCAATACTTTCATGTTCCGT22680.13799615947196392No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACGGAATCGGTCAGGGACCCCG22660.1378744697369798No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG22320.13580574424225017No Hit
GAACTACATGAGCTACTTAAGTTGGTATCAGCAGAAACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCGT22240.13531898530231382No Hit
GAACATGAAAGTATTGCTGACAATAATAAACTGCCACATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAAT22100.13446715715742513No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT21990.1337978636150126No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG21890.13318941494009215No Hit
GTCTCCAGATTCCCTGACTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATA21870.13306772520510804No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21610.1314857586503148No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA21190.12893027421564882No Hit
TTCATGTTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAGATCAAACGA21150.12868689474568062No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21150.12868689474568062No Hit
GCTCATTTACTGGGCGTCTACCCGGGCATCCGGGGTCCCTGACCGATTCCGTGGCAGCGGGTCTGGGACAGATTT20930.1273483076608556No Hit
CATCATGATGACGCAGTCTCCAGATTCCCTGACTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAG20850.12686154872091918No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT20790.1264964795159669No Hit
GGTCCAACAGTCAGAACTACATGAGCTACTTAAGTTGGTATCAGCAGAAA20640.1255838065035862No Hit
CCTTGGTCCCTTGGCCGAACGTCCACGGAACATGAAAGTATTGCTGACAATAATAAACTGCCACATCTTCAGG20440.12436690915374525No Hit
TCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACGGAATCGGTCAGGGACC19990.12162889011660309No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT19840.1207162171042224No Hit
GGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC19550.11895171594695302No Hit
GTCAGGGACCCCGGATGCCCGGGTAGACGCCCAGTAAATGAGCAGCTTAGGAGGCTGTCCTGGTTTCTGCTGATA19300.11743059425965183No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA19220.11694383531971543No Hit
GTTTATTATTGTCAGCAATACTTTCATGTTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAGATCAAACGAA19200.11682214558473136No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA19090.11615285204231883No Hit
GTCCTGGTTTCTGCTGATACCAACTTAAGTAGCTCATGTAGTTCTGACTGTTGGACCTGTATAAAACACTCTGG18960.1153618687649222No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT18950.11530102389743016No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG18470.11238047025781187No Hit
GGATCTCTGGTGCCTACGGGGACATCATGATGACGCAGTCTCCAGATTCCCTGACTGTGTCTCTGGGCGAGAGGG18340.11158948698041526No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC17990.1094599166181936No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA17970.1093382268832095No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC17570.1069044321835276No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17480.10635682837609917No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC17430.10605260403863893No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT17270.10507908615876617No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC17240.10489655155629No Hit
GTAAATGAGCAGCTTAGGAGGCTGTCCTGGTTTCTGCTGATACCAACTTA17220.10477486182130594No Hit
CACCATCAGCAGCCTGCAGCCTGAAGATGTGGCAGTTTATTATTGTCAGCAATACTTTCATGTTCCGTGGACGTT17220.10477486182130594No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT17070.10386218880892521No Hit
ATAATAAACTGCCACATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGA16970.10325374013400473No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG16960.10319289526651268No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC16900.1028278260615604No Hit
GACCGATTCCGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAG16890.10276698119406835No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)