FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689199

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689199
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences873019
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT28170.32267338969713144No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25920.29690075473729666No Hit
GGTCAGGATATTGCCAGCTGGTTAGCCTGGTATCAACAGAAACCAGGGAAAGCCCCTAAGCTCCTAATCTATG20670.2367646064976822No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19460.22290465614150434No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19220.220155575079122No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18520.2121374219805067No Hit
GGATATTGCCAGCTGGTTAGCCTGGTATCAACAGAAACCAGGGAAAGCCCCTAAGCTCCTAATCTATGCTGCATC17540.20091200764244538No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17050.19529930047341468No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGGGTCAGGATATTGCCAGCTGGTTAGCCTGGTATCAACAGAAA15860.18166844020576872No Hit
AATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGCGACAGATTTCA15670.179492084364716No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15490.17743027356792923No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15460.17708663843513142No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAACTCCTGGGGCTC15240.1745666474612809No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15190.17399392223995125No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14640.16769394480532498No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14250.1632266880789536No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14220.16288305294615582No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14050.16093578719363497No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC13420.15371944940488122No Hit
GCATGGACATGAGGGTCCCCGCTCAACTCCTGGGGCTCCTGCTGCTCTGG13330.15268854400648785No Hit
ACTTACTACTGTCAACAGGTTCTCAGTTTCCCTCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA13250.15177218365236037No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12300.1408904044470968No Hit
CTCCTAATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTT12210.13985949904870343No Hit
CAGCATGGACATGAGGGTCCCCGCTCAACTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT12170.13940131887163967No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCGGAGGGAAA12080.1383704134732463No Hit
GTAATATGTGGCTGTGTCCACAGGGTCCATGTTGGTCATTGTAAGGACCA11960.1369958729420551No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11790.13504860718953424No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT11100.12714499913518493No Hit
GTAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCGCAGATCCACTGCCG10830.12405228294000473No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10680.12233410727601576No Hit
GATTAGGAGCTTAGGGGCTTTCCCTGGTTTCTGTTGATACCAGGCTAACC10590.12130320187762236No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10570.1210741117890905No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10400.11912684603656964No Hit
ATCCTGACCCGCCCGACAAGTGATGGTGACTCTGTCTCCTACAGATGCAGACACGGAAGATGGAGACTGGGTCAT10390.11901230099230371No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10340.11843957577097407No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10250.11740867037258068No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT10100.11569049470859169No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10080.11546140462005983No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10050.11511776948726202No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG10020.11477413435446422No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAACTCCTGGGGCTCCTGCT9590.10984869745102914No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC9350.10709961638864676No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9250.10595416594598743No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9160.10492326054759404No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG9130.10457962541479623No Hit
CACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTACTGTCAACAGGTTCTCAGTTTCCCTCCGACGTT9060.10377781010493471No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAACTCCTGG8900.1019450893966798No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGCGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTG8810.10091418399828642No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG8740.10011236868842488No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAT408.4342355E-751.59639439
TGTCGTA408.5397005E-751.50355538
GGTACGA306.900956E-445.1726262
GTACGAC350.001473383938.719393
ATGTCGT557.73007E-637.39201737
CGTATGC3350.037.0961741
GACAGGT1200.036.7216871
ATGCCGT3500.036.6766944
GTGGTAT1250.035.252821
GTATGCC3600.034.572542
TCGTATG3700.034.4657440
GCCGTCT3650.034.35592746
CACGAGA3750.033.446921
ATCTCGT2900.033.09408637
CTTATAC3900.033.027791
ACGAGAC3800.033.0068122
GATCTCG2950.032.4917536
CCGTCTT3950.031.7990447
TATACAC4200.031.4595073
ATCTCCG4050.030.95161210
Produced by FastQC (version 0.11.5)