FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689200

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689200
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences873019
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32070.36734595696084504No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT28340.3246206554496523No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22040.2524572775621149No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20070.22989190384172625No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19670.22531010207108895No Hit
GGTCAGGATATTGCCAGCTGGTTAGCCTGGTATCAACAGAAACCAGGGAAAGCCCCTAAGCTCCTAATCTATG19110.21889557959219674No Hit
GGATATTGCCAGCTGGTTAGCCTGGTATCAACAGAAACCAGGGAAAGCCCCTAAGCTCCTAATCTATGCTGCATC16100.18441752126815109No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16060.18395934109108739No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16060.18395934109108739No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14690.16826667002665463No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGGGTCAGGATATTGCCAGCTGGTTAGCCTGGTATCAACAGAAA14350.16437213852161292No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14330.16414304843308106No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13930.15956124666244378No Hit
AATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGCGACAGATTTCA13620.15601035029019986No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC13310.15245945391795598No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13220.15142854851956258No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13140.15051218816543513No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13120.15028309807690327No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13040.14936673772277578No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAACTCCTGGGGCTC13020.14913764763424392No Hit
ACTTACTACTGTCAACAGGTTCTCAGTTTCCCTCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA12420.14226494497828798No Hit
CTCCTAATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTT12420.14226494497828798No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12290.14077585940283086No Hit
GCATGGACATGAGGGTCCCCGCTCAACTCCTGGGGCTCCTGCTGCTCTGG11730.13436133692393865No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11440.1310395306402266No Hit
GTAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCGCAGATCCACTGCCG11020.12622863878105745No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCGGAGGGAAA10790.12359410276294103No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10790.12359410276294103No Hit
GTAATATGTGGCTGTGTCCACAGGGTCCATGTTGGTCATTGTAAGGACCA10770.12336501267440915No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10650.12199047214321795No Hit
CAGCATGGACATGAGGGTCCCCGCTCAACTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT10620.12164683701042017No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10340.11843957577097407No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT10270.11763776046111252No Hit
GATTAGGAGCTTAGGGGCTTTCCCTGGTTTCTGTTGATACCAGGCTAACC9860.1129414136462093No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9830.1125977785134115No Hit
ATCCTGACCCGCCCGACAAGTGATGGTGACTCTGTCTCCTACAGATGCAGACACGGAAGATGGAGACTGGGTCAT9830.1125977785134115No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT9810.11236868842487964No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAACTCCTGGGGCTCCTGCT9580.1097341524067632No Hit
GTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGCGACAGATTTCACT9440.10813052178704015No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9270.1061832560345193No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9250.10595416594598743No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9010.10320508488360505No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8950.10251781461800946No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8790.10068509390975455No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA8790.10068509390975455No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATAG150.002435209568.4221836
CGCACAA200.00777230751.03864331
TCCGTAG200.00795449850.74037611
GTGGTAT2150.042.485181
TAACGGC759.458745E-1041.0533136
TTAACGG759.567884E-1041.00816335
CGGTACC852.7685019E-936.788445
AATGGTA2950.034.83303570
ACGGGAT601.5306021E-533.84436816
TCGTACC400.002858464133.8288542
TTTAACG1155.2750693E-1132.65553734
GCCAACG450.004209981331.25749451
TATACTG450.005086770730.0718155
ATCAACG20100.029.7912963
TCAACGC20250.029.735984
CAACGCA20400.029.684865
GTTTAAC1052.604611E-829.23401333
CGAAATT853.6777183E-629.0960553
CGCAGAG21200.029.0434178
ACGCAGA21350.028.6809067
Produced by FastQC (version 0.11.5)