FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689203

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689203
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1607621
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATAAGCCTGGCCAGCCTCCCAGGCTCCTCATCTATGATGCATTCAAAAAAGCCACTGGCGTCCCAGGCAGGTT53650.3337229359407472No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT49880.3102721350368028No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC45920.28563946353027236No Hit
GGCTTATGCTGGTACCAGGCTAAGTGCGTATAAATATCTTCACTGGCCCTGCAGGAGAGGGTGGCTGTTTCCC37800.23513004619869984No Hit
CACTTAGCCTGGTACCAGCATAAGCCTGGCCAGCCTCCCAGGCTCCTCATCTATGATGCATTCAAAAAAGCCA36710.22834984116281137No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33630.20919109665773214No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32120.19979833555296925No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30010.18667335149267147No Hit
GTCTTTGTCTCCAGGGGAAACAGCCACCCTCTCCTGCAGGGCCAGTGAAGATATTTATACGCACTTAGCCTGGTA28920.17989314645678303No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG27940.17379718229607602No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG27340.17006495934054108No Hit
GTAGTAAACTGCAAAATCTTCAGGCTCCAGGTTGTGGATAGTGAGAGTGAAGTCTGTCGCAGACCCACCGCCA26020.16185406883836428No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAACAGCCACCCTCTCCT25960.16148084654281075No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25790.16042338337207587No Hit
CTCCTGCAGGGCCAGTGAAGATATTTATACGCACTTAGCCTGGTACCAGCATAAGCCTGGCCAGCCTCCCAGGCT25790.16042338337207587No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25580.15911710533763865No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25180.15662895670061538No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24900.15488725265469908No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA24790.15420301177951767No Hit
GTTTACTACTGTCAGCAGGGCACCAACTGGCCCCCCTTCGTTTTCGGCCC24370.15159045571064322No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23840.1482936587665874No Hit
ATCCACAACCTGGAGCCTGAAGATTTTGCAGTTTACTACTGTCAGCAGGG23800.14804484390288508No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22990.14300634291291292No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22740.1414512500147734No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAACCTCCACTGTGGTCC22340.1389631013777501No Hit
CTCCTGTGTAGCCTCTCAATTCTCTTTCACCGACGCCTGGTTGACCTGGGTCCGCCAAGGTCCAGGAAAGGGC21340.13274272978519192No Hit
GTATTATTGTGCCGCAGGACTGAACGAGGTGGTCCCGGGGGCATATTACA21090.13118763688705234No Hit
GCTCAATTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT20350.1265845619085593No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT20180.1255270987378244No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG19250.11974215315674527No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT19100.11880909741786155No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG18610.11576111533750802No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18410.11451704101899639No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18390.11439263358714524No Hit
GTTCAGTGGCGGTGGGTCTGCGACAGACTTCACTCTCACTATCCACAACCTGGAGCCTGAAGATTTTGCAGTTTA18360.11420602243936849No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT18080.1124643183934522No Hit
GTCCAGGAAAGGGCCTGGAGTGGGTTGGCCGCATTAAGAGCGAGAGTCACGGCGGGGCAGCAGAGTACGCTGCAT18050.11227770724567546No Hit
GGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGATATTC17970.11178007751827079No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17780.11059820691568473No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17690.1100383734723545No Hit
CAATAATACACGCCAGAGTCTTCGGTTTTCAGGCTCTTTATGTGAAGATA17640.10972735489272659No Hit
CCACTGAACCTGCCTGGGACGCCAGTGGCTTTTTTGAATGCATCATAGATGAGGAGCCTGGGAGGCTGGCCAGG17620.10960294746087541No Hit
CAGTAGTAAACTGCAAAATCTTCAGGCTCCAGGTTGTGGATAGTGAGAGTGAAGTCTGTCGCAGACCCACCGCCA16870.10493766876645677No Hit
GTGCAGCCACAGTTCGTTTAACCTCCACTGTGGTCCCAGGGCCGAAAACGAAGGGGGGCCAGTTGGTGCCCTG16850.1048132613346056No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA16320.10151646439054976No Hit
CCCTGTATCTTCACATAAAGAGCCTGAAAACCGAAGACTCTGGCGTGTAT16240.10101883466314511No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16150.10045900121981487No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16090.10008577892426138No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16080.1000235752083358No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT451.3714889E-462.6610470
TCGTATG3000.046.0109840
TATCTCG3550.045.41280436
ATCTCGT3500.043.17707437
GACCGAT5550.042.91220526
CGTATGC3700.042.0265941
GTGGTAT3850.041.472841
ACGAGAC6150.041.4478522
ACCGATC5750.040.84444427
ATGCCGT3400.040.83151244
GGTATCA16000.040.5545921
CACGAGA6300.040.45343821
TATACAC6600.040.1330033
CCGATCA5950.039.50230828
AGACCGA6150.039.2690825
TCAGTAT6200.039.14018232
GAGACCG6600.038.63629524
ATCTCCG6700.038.5203110
CCCACGA6750.038.24925219
GTATGCC4350.038.19394342
Produced by FastQC (version 0.11.5)