FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689204

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689204
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1607621
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG70620.4392826418664598No Hit
GCATAAGCCTGGCCAGCCTCCCAGGCTCCTCATCTATGATGCATTCAAAAAAGCCACTGGCGTCCCAGGCAGGTT53390.3321056393266821No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT50280.3127602836738261No Hit
GGCTTATGCTGGTACCAGGCTAAGTGCGTATAAATATCTTCACTGGCCCTGCAGGAGAGGGTGGCTGTTTCCC37360.2323930826979742No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36510.22710576684429976No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33760.2099997449647647No Hit
CACTTAGCCTGGTACCAGCATAAGCCTGGCCAGCCTCCCAGGCTCCTCATCTATGATGCATTCAAAAAAGCCA33410.20782261490736934No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30840.19183625991449477No Hit
GTCTTTGTCTCCAGGGGAAACAGCCACCCTCTCCTGCAGGGCCAGTGAAGATATTTATACGCACTTAGCCTGGTA30420.18922370384562032No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29850.18567809203786215No Hit
GTAGTAAACTGCAAAATCTTCAGGCTCCAGGTTGTGGATAGTGAGAGTGAAGTCTGTCGCAGACCCACCGCCA27330.1700027556246155No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT26580.16533747693019685No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG26570.16527527321427127No Hit
CTCCTGCAGGGCCAGTGAAGATATTTATACGCACTTAGCCTGGTACCAGCATAAGCCTGGCCAGCCTCCCAGGCT26480.16471543977094105No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25440.1582462533146805No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAACAGCCACCCTCTCCT25080.15600691954135956No Hit
GTTTACTACTGTCAGCAGGGCACCAACTGGCCCCCCTTCGTTTTCGGCCC24650.15333215975655953No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24140.15015977024435487No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24080.14978654794880136No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23350.1452456766862339No Hit
ATCCACAACCTGGAGCCTGAAGATTTTGCAGTTTACTACTGTCAGCAGGG23200.14431262094735015No Hit
CTCCTGTGTAGCCTCTCAATTCTCTTTCACCGACGCCTGGTTGACCTGGGTCCGCCAAGGTCCAGGAAAGGGC22140.13771902705923847No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22070.13728360104775938No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21800.13560410071776868No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21500.13373798924000121No Hit
GTATTATTGTGCCGCAGGACTGAACGAGGTGGTCCCGGGGGCATATTACA20710.12882389568188024No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAACCTCCACTGTGGTCC20540.12776643251114536No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA20370.12670896934041045No Hit
GCTCAATTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT19700.12254132037339646No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19480.12117283862303366No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT19120.1189335048497127No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC18760.11669417107639177No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18380.11433042987121964No Hit
GTTCAGTGGCGGTGGGTCTGCGACAGACTTCACTCTCACTATCCACAACCTGGAGCCTGAAGATTTTGCAGTTTA17890.11128244779086613No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17740.11034939205198241No Hit
GTCCAGGAAAGGGCCTGGAGTGGGTTGGCCGCATTAAGAGCGAGAGTCACGGCGGGGCAGCAGAGTACGCTGCAT17470.10866989172199168No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17420.10835887314236377No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17030.1059329282212661No Hit
GTGCAGCCACAGTTCGTTTAACCTCCACTGTGGTCCCAGGGCCGAAAACGAAGGGGGGCCAGTTGGTGCCCTG16960.10549750220978701No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG16800.10450224275497769No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA16120.10027239007203811No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)