FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689205

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689205
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1205639
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT52370.4343754639655817No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC48090.39887561699646407No Hit
GATTTTACACTGAGAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAGGCGCTACAAACT37460.31070660454746407No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG35640.29561087522882057No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34370.28507704213284407No Hit
GTAATGGATACGACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCCCCACATCTCCTGATCTATTTGGGTT33700.27951982309795886No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32240.26741006221597013No Hit
GGTCTAGTCAGAGCCTCCTGCATAGTAATGGATACGACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCCC29310.24310759688430783No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG29290.24294170974893814No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTCTCAGTGTAAAATCTGTGCCTGACCCACTGCCG29210.2422781612074593No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG29180.24202933050440473No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG28890.23962396704154393No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA28520.2365550550372043No Hit
GTGTAAAATCTGTGCCTGACCCACTGCCGCTGAACCTGTCAGGGACCCCG27200.22560650410280358No Hit
CTCCTACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGC26690.221376382150876No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26430.2192198493910698No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT26380.2188051315526455No Hit
AGCCAGGGCAGTCCCCACATCTCCTGATCTATTTGGGTTCTAATCGGGCC26110.21656565522515445No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25900.21482384030377255No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25740.21349674322081483No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG25560.2120037590024875No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG25320.21001311337805095No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGA25070.2079395241859296No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT24990.20727597564445077No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24740.2052023864523294No Hit
CTCCTGTACAGCGTCTGAATTCAGCATCAGTAACTATGGCATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGG24150.20030871595892305No Hit
AGGTAGGAGTTTGTAGCGCCTGCATGCAGTAATAAACCCCAACATCCTCA22930.1901896007013708No Hit
CTACAAACTCCTACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCAT22150.1837200024219522No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG21340.17700157343947898No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGTCCGTCACCC20930.17360088716439998No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCC20790.172439677216812No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20610.17094669299848464No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC20610.17094669299848464No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20400.1692048780771027No Hit
GTCTCCACTCTCCCTGTCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT19450.16132523914704153No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT19310.16016402919945355No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19160.15891987568418076No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA18920.15692923005974424No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTAGGAGTTTGTAG18720.15527035870604716No Hit
GTTTATTACTGCATGCAGGCGCTACAAACTCCTACCTTCGGCCAAGGGAC18520.15361148735235008No Hit
GCCCTGGCTTCTGCAGGTACCAATCCAAATAGTCGTATCCATTACTATGC18510.15352854378466524No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAACCCAAATAGATCAGGAGATGTGGGGACTGCCCTGGCTTCTGCAGGTA18150.1505425753480105No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17170.14241410571489477No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17140.14216527501184018No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17110.1419164443087856No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACT16440.1363592252739004No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT16420.13619333813853068No Hit
GTCCCTGACAGGTTCAGCGGCAGTGGGTCAGGCACAGATTTTACACTGAG16400.13602745100316097No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16310.1352809588939973No Hit
CTCCAGGGGTGACGGACAGGGAGAGTGGAGACTGAGTCATCACAATATCC16270.1349491846232579No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15870.1316314419158637No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15790.13096789337438486No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC15510.12864547347920896No Hit
GTCCCCACATCTCCTGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCC15290.12682071499014214No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT15250.12648894071940273No Hit
GGATACGACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCCCCACATCTCCTGATCTATTTGGGTTCTAAT15190.12599127931329362No Hit
GTCGTGTCCCTTGGCCGAAGGTAGGAGTTTGTAGCGCCTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCA15190.12599127931329362No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15180.12590833574560875No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT15150.1256595050425542No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15130.1254936179071845No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14650.12151232665831148No Hit
ATCCATTACTATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCC14430.11968756816924468No Hit
GCATAGTAATGGATACGACTATTTGGATTGGTACCTGCAGAAGCCAGGGC14230.11802869681554762No Hit
GAAGTACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAAGTACA14190.11769692254480818No Hit
GAGTTTGTAGCGCCTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTCTCAGTGTAAAAT14070.11670159973258994No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA13950.11570627692037169No Hit
CTCCCTGTCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA13950.11570627692037169No Hit
GACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGTGACGGACAGG13810.11454506697278373No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT13620.11296913918677148No Hit
CGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT13550.11238853421297751No Hit
AGTCAGAGCCTCCTGCATAGTAATGGATACGACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCCCCACAT13470.11172498567149867No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13260.10998317075011674No Hit
GGGATATTGTGATGACTCAGTCTCCACTCTCCCTGTCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGT13230.10973434004706217No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT13180.1093196222086379No Hit
TTATTACTGCATGCAGGCGCTACAAACTCCTACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGG13100.10865607366715908No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13100.10865607366715908No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC13040.10815841226104994No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12980.10766075085494081No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG12950.10741192015188628No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12940.1073289765842014No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG12940.1073289765842014No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12830.106416597339668No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12740.10567010523050432No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12600.10450889528291636No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12460.1033476853353284No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT12450.10326474176764355No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT12420.10301591106458899No Hit
GGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGTCCGTCACCCCTGGAGAGCCGG12260.1016888139816313No Hit
GAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAGGCGCTACAA12260.1016888139816313No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTCTCAGTGTAAAATCTGTGCCTGACCCACTGCCG12120.10052760403404334No Hit
GAGAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAGGCGCTACAAACTCCTACCTTCGG12100.10036171689867365No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA12060.10002994262793424No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGCC150.002476382368.135132
CGTCTTA200.007007073652.3954748
TCGTATT200.00742005151.6421340
TCGTATG5400.045.90411840
CGCGTAT306.4379454E-445.81525439
CGTATGC5800.045.78927641
ATGTCGT1500.045.6653737
ATGCCGT5750.044.63112644
CTCTTAT1450.044.3938941
GGTATCA10950.043.935121
CCGTCTT5650.043.8085247
ACGAGAC6850.043.52951422
TTATACA7050.043.232552
CACGAGA7000.043.0736421
AGCGATG1900.043.06863433
GTATGCC6250.042.56976742
AAAGGGG4650.042.48888868
CGGGATA405.5797587E-542.33362617
GAGCGAT6050.042.23249432
TATACAC7300.042.2158933
Produced by FastQC (version 0.11.5)