FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689209

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689209
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98
Sequences flagged as poor quality0
Sequence length29-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT22.0408163265306123No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGC11.0204081632653061No Hit
CAGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACACCTC11.0204081632653061No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA11.0204081632653061No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11.0204081632653061No Hit
CGCCAACGCAAGGCATCAGCAGCAGCCAGAGAAGGGCAGTCGGTACTTGATTAAGCTTAACAGTGATGACATCCT11.0204081632653061No Hit
GGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGTAGTTGAGGGAACTGGCCAGGGCACAGTCACAAC11.0204081632653061No Hit
AGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCATCCCTCACCCCCCACCACGGGA11.0204081632653061No Hit
GTACTATCCCTACTACTAGGTATCAATACTTACTACAAATGTATAATTATTAAATCAGTATGGCAGTGGCGTAGA11.0204081632653061No Hit
AAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTC11.0204081632653061No Hit
GTGCAGTTACAAAATGGATTGTTTCAAATGGCAATTTCTTACACTAACCTGATTATGAAAAAAAGAAGTCTGTA11.0204081632653061No Hit
GCACAGCCCAAACTCCATGGCGAGTTATATATTATCATTTCTAGAATACCAATCTCCTACCCCACGAAAACGCCG11.0204081632653061No Hit
AATGAACCATAACTGATTTAATGAGAAATTCGCAGTTTCACTGTACCGGA11.0204081632653061No Hit
ATATCACCCCGTAGAAACGGTTAGTAAGCAAGCGACCACACACCGGAAGG11.0204081632653061No Hit
GAACAGCGCTCTTCCCACTTGAGGGCGTCCAGGTGAAGGTGACACCTGAG11.0204081632653061No Hit
TTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGGC11.0204081632653061No Hit
GCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGGGATGCCCAAGTCAGGTACTTCTCGCGGGGCAGCTC11.0204081632653061No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGAGCTCGTTTGGCGT11.0204081632653061No Hit
CCTGAGAAGCGATCAGGGATCCCGTCCCCCTTGGTGAGGCTGCCATC11.0204081632653061No Hit
CAAAAGATATGACAATGAACTATAACTAACCATAACCAGACAAAATTCAA11.0204081632653061No Hit
GTAGCAGCCACACAGGTCACGGTAACGATGTCCTAGACCACAGCAATAACCAATATAGGGAGTCACTTTGAATGT11.0204081632653061No Hit
CAGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACG11.0204081632653061No Hit
GACTTCGACAAACACAAAGTCAACCACTCCGCAGAATACCATAAGTCCAAGAGGAAGCACGACACAAACACCTA11.0204081632653061No Hit
CCCTGGCCCTATGCCTGCAACACTGTCACCTGCACGTGGATTGGGAACCA11.0204081632653061No Hit
GTCCTGCCTGGCTGTGCCCAGCCATGGAACCATGGGGAGACCTTCACCTG11.0204081632653061No Hit
GCTATGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGG11.0204081632653061No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT11.0204081632653061No Hit
TATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGA11.0204081632653061No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTTTTTTTTTT11.0204081632653061No Hit
GCCTCAGAGAGTCAGTTCCGCGGTAGAGGTGACCTGACTCTCTGAGGCTCATTTTGCAGTTGTTGAAATTGTCCC11.0204081632653061No Hit
ACTCTTGTACAACACTACACCGAAGACATCCTAGCAGACACGGAAGATAAAGTCTGTCACTCCCTGTCCTCAT11.0204081632653061No Hit
AGGCTGCAGGCTGAAGATGCGGCTGTTTATTACTGTCAGCAATATTATCT11.0204081632653061No Hit
GAGCAAGAGTTGAAAAGTGGATTGCTGACCATTCAAAGAGTGATCGAATGACTCGAGGACTCCGAGCCAAAGTAG11.0204081632653061No Hit
GCAAACGGCCTTTCCCAGCATTCACCTGATACCAGGCCAACCACGTGCTA11.0204081632653061No Hit
GCAACATGAAGGTGCTCCTTGCCGCCGCCCTCATCGCGGGGTCCGTCTTCTTCCTGCTGCTGCCGGGACCTTCTG11.0204081632653061No Hit
GGGCCGGCGTGATATTGATATAGAATTGCTAATTATAAGATGCATCTACA11.0204081632653061No Hit
GAGCTGCAGGATCCCAGGGGAGGGGTCTCTCTCCCCATCCCAAGTCATCCAGCCCTTCTACCTGAACTCATGAAA11.0204081632653061No Hit
GAGAAGGGCTTGATGCCTTGGGGTGCGAGGAGAGACCCCTCCCCTGGGATCCGGCAGCTCTAGTCTACTGTGGTG11.0204081632653061No Hit
GTGATTCACGTGGCATGTCATGGACTTGCCGGCTAGGCACTGTGTGGCCG11.0204081632653061No Hit
CTGCTGCACGGACTCTGGAACCATGAACATTTTTGATCGAAAGATCAACT11.0204081632653061No Hit
GAATAAAACACTCTGGCTGGACTTGCACCTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA11.0204081632653061No Hit
CACACGCACAGAACTATTATATTTTTTTAAATTGAAAATTATTAATAATTTAACGGTGCGAACAAACTGG11.0204081632653061No Hit
ACACTTCACACACAACACCATCGAAAGCAACCTCCTAAAAAACAACCAATGAAAATTATAAAAATAAAAGAACCA11.0204081632653061No Hit
GCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTA11.0204081632653061No Hit
TCTTATACACATCTCCGAGCCCACCAGACTAGGCATGAGGTCGTATGCCGTCTACTGCTTGAAAAAAAAAAGAGG11.0204081632653061Illumina Paired End PCR Primer 2 (95% over 21bp)
AAATAAACATAAACATCCTCCATTATGATTATTAACCAGAATAATAATGAGAGACGTGTGG11.0204081632653061No Hit
CCTTTGGGGTCCTGAGTCTTATCTGAGGC11.0204081632653061No Hit
CTACATCAATAACCTTACATCACAAGATTAAAAAGGAAAACAACACAAAAGAAGTCAGGCTCACATACATGAA11.0204081632653061No Hit
GTATAAAGCACTCCAATCCCGAAAATCCCAGGATACTATCACACATCAGGACAACAATCACAGCACCAACA11.0204081632653061No Hit
AATTAACTATATATGAAAACACATGAAAAATCAATTATAAAGATAACTAAAACCACTCATTCACAAAATTAAAAG11.0204081632653061No Hit
CTGTCTTTGTCTCCACGGGAAAGTGTCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGAAGCAACTACGTAGC11.0204081632653061No Hit
ATTTCCACCTTGGTCCCTTGGCCGAACGGCCGAGCAGTATAATATTATTGACAGTAATAAACTGCCACAT11.0204081632653061No Hit
ATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGCGGGGAAATATAATATTG11.0204081632653061No Hit
CACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGACC11.0204081632653061No Hit
GCCTCCCTAGTAGCTGGGACTACAGGTGCCCGCCATGACGCCCGGCTAAT11.0204081632653061No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGCAATCTCGTATGCCGTC11.0204081632653061No Hit
GTCTTACTGAGTTAAGACTTGGGGTGATTTGTTACAGACCCCAGTGTCGT11.0204081632653061No Hit
CCACCTACCCAATATCCCTCATGCTGCCCCCCCCGACTCTCACTGCACCAACCCTCCATCGACGACCTGCTCTAA11.0204081632653061No Hit
GTTGAAAATATTGATATTCAAAGAAACAAACAGCTTTGGATCCATAGCCA11.0204081632653061No Hit
TTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTT11.0204081632653061No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC11.0204081632653061No Hit
GTAAACTACTTAACAAACTTGTTACAAACCACATAGCACAACAAAAATAAAAAAGAAAAACCAAGGTT11.0204081632653061No Hit
GTGATCCACTGGGTGTCAGGATTTCTTCTGATAGCTTTATGGAATGGATC11.0204081632653061No Hit
CTGTAGCAGCCACAGAGGTCACGGTCAGGTGGTCCTTGAACAGCGCTCTT11.0204081632653061No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC11.0204081632653061No Hit
GTTATCCAACAACCACAGAACTTTCGCCTCTCTGGGATATAAGGTAGTCA11.0204081632653061No Hit
AAAAATAAATTAACAATATTTAAGCAAATAATAAAACTACACATTTAATATTTAATAAAAAAGAAAAAAAATA11.0204081632653061No Hit
CCTCCTCCACTGCACAGGGTCTCTCTCCCAGCT11.0204081632653061No Hit
TCAACAACCATCAGCATGTTCAATGTCAGAATGAAAACAATCCCATAACCCACGACACTTAAAAGAAAAGGGACA11.0204081632653061No Hit
CAATTACATATACAATTACCACAATTAACCAAATATAATACAAGACAACC11.0204081632653061No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTAGCTGTCCTGCTCT11.0204081632653061No Hit
GAATCATATTAACAATCGACCAAAGAAAACATAACTTATATAAAAATCCA11.0204081632653061No Hit
AAAAACGCAGAGTACGGGGAGAGCTCTCGGTAGTCTGCACCATGGCTTGGC11.0204081632653061No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTG11.0204081632653061No Hit
CTCTCGGAGCTCGGAGGACACGAAGCAAATGATGAGTAGCTTAATGCTGT11.0204081632653061No Hit
GTGGATAAAGAAATACAAACAAGTAACTCCCAGGAGAGTGTCAAAGACCACGACAGCAAGGACAGCACCTACAGC11.0204081632653061No Hit
GGTCACAGGAGCTGCCCCGCGAGAAGTACCTGACTTGGGCATCCCGGCAG11.0204081632653061No Hit
GTGGCGCAGCGCAGCACAGCACCGTCAGCACCTGCCTCCTCAGCGGCTCC11.0204081632653061No Hit
AAGAAGAAGTACAAGTGAAAGTATTACAATAGTTAAATGGCAAGGCAAAACACACAACCACACTGGAAAAC11.0204081632653061No Hit
AGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA11.0204081632653061No Hit
AAAAAAAACAAAAACCATCAAGATCCCCCAACCGACAATCAATGAACACAAACGACCTCTAGCACCACCAAA11.0204081632653061No Hit
GTAATACACAGCCGTGTCCTCGGTTCTCAGGCTGTTCATTTGCAGAGTCA11.0204081632653061No Hit
GAGCATAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11.0204081632653061No Hit
CAGCTGAACCTGCGGGAGTCGGCCACCATCACGTGCCTGGTGACGGGCTT11.0204081632653061No Hit
AAACAAAGCATAGCGACAATCTTTATCAGGAAGCAATCCCACAAAATGCT11.0204081632653061No Hit
ACAAGACAAAAAGCAATTAAACAAAATAATAGCATACTGTGACAAATGAAACCAAAAAAAAAAAAAAAAAAA11.0204081632653061No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT11.0204081632653061No Hit
GTCTTGCAGATCTGTGTGTTCCGGTCCCAATATTCCGGCCCCTCCTGCTCTATCCACGGCGCCCGCGGCTCCTCT11.0204081632653061No Hit
GGTGAATCCAGAGGCTGCACAGGAGAGTCTCAGGGACCCCCCAGGC11.0204081632653061No Hit
GGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGC11.0204081632653061No Hit
CACCTATAACAGACCTCAACCAAATATATCACCTCAACCACAACCTCAAACATGACTAAAATAAAACTAATGTAA11.0204081632653061No Hit
CCATGGGAAGACCTTCACTTGCACTGATGCCAACCCCGCGTCCAAGAACACGCAAACCGCCACCATCTCAAAATA11.0204081632653061No Hit
GGACACCAAAAGCTCCACGATGCCCAGGTAGCCATGGATAGAGGCTAAGT11.0204081632653061No Hit
GGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGAAGGACACTGGACACG11.0204081632653061No Hit
ACAGGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT11.0204081632653061No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT11.0204081632653061No Hit
GTAATGCGAGGAACATTTTAATACACATCCTGAATTAAACACCTATAGTA11.0204081632653061No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)