FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689211

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689211
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1044993
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGCTGCTTCACCGTGGACGTTCGGCCAAGGGA51640.49416598962863867No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31140.29799242674352844No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29840.2855521520239848No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA23350.22344647284718655No Hit
GTGTATTACTGTCAGCAGTATGCTGCTTCACCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA22140.21186744791591908No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT20770.1987573122499385No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20240.19368550794120154No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19400.185647176583958No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18890.1807667611170601No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18290.17502509586188617No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA17750.1698575971322296No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17540.1678480142929187No Hit
GAAATACACAGCCGTGTCCTCGACTCTCAGGCTGTTCATTTGCAGATACA17160.1642116262979752No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16460.15751301683360558No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT15780.1510057962110751No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15490.14823065800440768No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15340.1467952416906142No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15300.14641246400693592No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT15300.14641246400693592No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15240.14583829748141852No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG15170.14516843653498157No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15090.14440288116762506No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15030.14382871464210767No Hit
CTCCTGCAGGGCCGGTCAGAGTGTTAGCAGCAGTTACTTTGCCTGGTACC14720.1408621875936011No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG14310.1369387163358989No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14030.13425927255015105No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT13730.13138843992256408No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13500.12918746824141406No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT13430.12851760729497708No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13360.12784774634854015No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA13360.12784774634854015No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13330.12756066308578146No Hit
CTCCTGTGCAGCGTCTGGATTCATTTTCAGTAACTATGGCATGCACTGGGTTCGCCAGGCTCCAGGCAAGGGG12970.12411566393267706No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12160.11636441583819221No Hit
GTCTTTGTCTCCAGGGGATAGAGCCACCCTCTCCTGCAGGGCCGGTCAGA11990.1147376106825596No Hit
GTGTATTTCTGTGCGACAGATCGTGGGAGTTCCCCCTTTGACTACTGGGGCCAGGGAACCCCGGTCACCGTCTC11800.11291941668508784No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC11780.11272802784324872No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGTT11460.1096658063738226No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11450.10957011195290303No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC11390.10899594542738564No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC11210.10727344585083345No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGATAGAGCCACCCTCTCCTGCAGGGCCGGTCAGAGTGTTAGCAG11140.10660358490439648No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT11030.10555094627428127No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10980.10507247416968345No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGCTGCTTCA10780.10315858575129211No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10670.1021059471211769No Hit
CATCTACGGTGCATCCAACAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA10530.10076622522830296No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTCGA150.002331625269.17728441
TCGTATG4800.049.64337540
ATGCCGT5350.049.40383544
CGTATGC5300.048.9461941
CTTGAAA5300.048.4793657
ATCTCGT4550.048.29735637
GCCGTCT4800.048.00370846
CTAGCGC6800.047.8836228
CGTCTTA305.850374E-446.71299448
TATACAC7400.046.581233
TAGCGCT6950.046.40671529
CACGAGA6800.046.2517721
ACGAGAC6950.046.23336422
CATCTCG5050.046.15437736
ATCTCCG7050.046.01766610
CGCGTAT451.8797746E-645.93372739
ACTAGCG7050.045.66066427
GCGCTCA6950.045.03215831
CGCTCAT6300.044.87685832
CCGTCTT5400.044.682447
Produced by FastQC (version 0.11.5)