FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689212

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689212
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1044993
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG58720.5619176396396913No Hit
GTATTACTGTCAGCAGTATGCTGCTTCACCGTGGACGTTCGGCCAAGGGA47180.45148627789851226No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31960.30583936925893285No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA23400.22392494495178436No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23110.22114980674511694No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21480.20555161613522768No Hit
GTGTATTACTGTCAGCAGTATGCTGCTTCACCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA20740.1984702289871798No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20220.19349411909936237No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16800.16076662714487083No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16450.15741732241268602No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16300.15598190609889254No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA16000.15311107347130554No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG15240.14583829748141852No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15140.14488135327222287No Hit
GAAATACACAGCCGTGTCCTCGACTCTCAGGCTGTTCATTTGCAGATACA15020.14373302022118808No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14300.13684302191497932No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT14180.13569468886394453No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14090.13483343907566844No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14060.13454635581290975No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13790.13196260644808147No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13760.13167552318532277No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13760.13167552318532277No Hit
CTCCTGCAGGGCCGGTCAGAGTGTTAGCAGCAGTTACTTTGCCTGGTACC13360.12784774634854015No Hit
CTCCTGTGCAGCGTCTGGATTCATTTTCAGTAACTATGGCATGCACTGGGTTCGCCAGGCTCCAGGCAAGGGG13300.12727357982302276No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA13200.12631663561382708No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC12740.12191469225152705No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12430.11894816520302051No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12180.11655580468003135No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT12140.11617302699635308No Hit
GTGTATTTCTGTGCGACAGATCGTGGGAGTTCCCCCTTTGACTACTGGGGCCAGGGAACCCCGGTCACCGTCTC12050.11531177720807699No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11800.11291941668508784No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11790.11282372226416827No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGAACCCAGTGCATGCCATAGTTAC11580.11081413942485739No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11500.11004858405750087No Hit
GTCTTTGTCTCCAGGGGATAGAGCCACCCTCTCCTGCAGGGCCGGTCAGA11440.10947441753198348No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGTT11210.10727344585083345No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11190.10708205700899433No Hit
GGGCTGGAGTGGGTGGCAGTTATATGGGCTGATGGAAGTAAAGACATTTA11090.10612511279979867No Hit
GTATGCTGCTTCACCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT11020.10545525185336169No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT10740.10277580806761384No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG10650.10191455827933775No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT10610.10153178059565948No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGCTGCTTCA10580.10124469733290078No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT10550.10095761407014209No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCCG208.773622E-568.5822937
ACGTATT252.191844E-456.99377456
TACGTAT305.4555363E-447.37980755
GGTATCA14250.040.47361
GTATCAA34900.037.7869571
AACTGTG20650.037.12641570
CCTAACG601.5679254E-533.7263832
GTAGTTT1801.1145494E-732.76326870
TCTGGAG8200.032.36371670
GTACGTA450.0040436431.51658854
CGCATAA450.004161540431.33207551
CCGCATA450.004195281331.28042850
TATCAAC42600.030.7970962
AACGCAG43150.030.5652626
ATCAACG43500.030.0838263
AATACGG450.005162272629.981885
TCAACGC43850.029.847994
CAACGCA44400.029.5513975
CGCAGAG45100.029.3184998
ACGCAGA45200.029.2536337
Produced by FastQC (version 0.11.5)