FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689213

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689213
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences833792
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT28050.33641483727356464No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25870.3102692278170095No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAGACCA19470.2335114752840037No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19070.2287141157506908No Hit
GTAGTAGGCGGCCGTGTCCGCAGTGGTCACAGAGGTCAGCCTCAGGGAGAACTCATTCTTGGACGTGTCTACGGA18420.22091840650905742No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18340.21995893460239482No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18040.21636091495241017No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16430.1970515428308259No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16030.19225418329751306No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15870.1903352394841879No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15590.1869770878108689No Hit
GTCTTTGTCTCCGGGGGAAGGAGCCACCCTCTCCTGCAGGGCCAATCAGAGTGTTACCGGCTACTTAGCCTGGTA15580.1868571538225361No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCGGCCTAGAGCCTGAAGATT15080.180860454405895No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14670.1759431608842493No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14430.1730647451642616No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14120.16934679152594412No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13760.16502916794596253No Hit
GACTTCACTCTCACCATCAGCGGCCTAGAGCCTGAAGATTTTGCAATTTATTATTGTCAACACCGTGGCGACTGG13730.16466936598096407No Hit
GTTCAGGGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCGGCC13710.16442949800429843No Hit
GTCCCAGACCCACTGCCCCTGAACCTGCCCGGGATGCCAGTGGCCCTGTT13600.1631102241326374No Hit
GAGTGTTACCGGCTACTTAGCCTGGTACCAACACAAACCTGGCCAGGCTCCCAGGCTCCTCATCTTTGATACAT13470.16155108228431073No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT13080.15687365673933065No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11840.1420018421860608No Hit
GTCTCCAAGCACCCTGTCTTTGTCTCCGGGGGAAGGAGCCACCCTCTCCTGCAGGGCCAATCAGAGTGTTACCGG11570.1387636245010746No Hit
CTCCTGCAGGGCCAATCAGAGTGTTACCGGCTACTTAGCCTGGTACCAACACAAACCTGGCCAGGCTCCCAGGCT11470.1375642846177464No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCCCTGAACCTGCCCGGGATGCCA11330.1358852087810869No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11210.13444600092109302No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT10990.13180745317777096No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC10750.12892903745778322No Hit
ACACAAACCTGGCCAGGCTCCCAGGCTCCTCATCTTTGATACATCTAACAGGGCCACTGGCATCCCGGGCAGGTT10500.1259306877494627No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10340.12401174393613754No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10250.12293233804114216No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10160.12185293214614676No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT10110.12125326220448265No Hit
GGTTTGTGTTGGTACCAGGCTAAGTAGCCGGTAACACTCTGATTGGCCCT9970.11957418636782316No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9840.11801504451949647No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9840.11801504451949647No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC9670.1159761667178385No Hit
GTCGTGTCCCTTGGCCGAAGGTGGGCCAGTCGCCACGGTGTTGACAATAA9630.11549643076450722No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC9600.11513662879950874No Hit
CCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCA9590.11501669481117592No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG9440.1132176849861836No Hit
GCCTACTACTGTGCGAGAGGCAGAGGGCGTTACTACGGTTTCGACGTCTGGGGCCAGGGGACCACGGTCATCGT9430.11309775099785079No Hit
GTACCAACACAAACCTGGCCAGGCTCCCAGGCTCCTCATCTTTGATACATCTAACAGGGCCACTGGCATCCCGGG9080.10890006140620204No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8850.10614157967454713No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT8820.10578177770954866No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8800.10554190973288302No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC8790.1054219757445502No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8710.10446250383788762No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA8680.10410270187288917No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT8620.10338309794289224No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT8480.10170402210623274No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT8480.10170402210623274No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT8460.10146415412956708No Hit
GTGTTGACACAGTCTCCAAGCACCCTGTCTTTGTCTCCGGGGGAAGGAGC8460.10146415412956708No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8420.1009844181762358No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT8350.10014488025790605No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG8350.10014488025790605No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCGG300.00218129870.3419270
CCGTCCG200.006433842253.53693863
ATCTCGT2950.046.61252237
CGTATGC3350.046.38776841
GGTATCA9700.045.434951
ATGCCGT3500.044.58360344
ACGAGAC3650.043.04858422
CGAGCCC3750.042.80123515
TCGTATG3550.042.74457640
CTTATAC3550.042.3444441
CACGAGA3750.041.90062321
CCGTCTT3850.041.53494647
CGTCTTC3800.041.21697648
TATACAC3900.041.147633
GCCGTCT3950.040.44638446
TTATACA3800.040.433422
GTATGCC3850.040.4320542
AGAGCGG700.001225097740.1953870
GCACATT350.001418137339.021466
ATCTCCG4150.038.67117710
Produced by FastQC (version 0.11.5)