FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689214

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689214
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences833792
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT28970.3474487642001842No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27220.32646031624194044No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22360.2681723979121891No Hit
GTAGTAGGCGGCCGTGTCCGCAGTGGTCACAGAGGTCAGCCTCAGGGAGAACTCATTCTTGGACGTGTCTACGGA22080.2648142462388701No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAGACCA19710.2363898910039914No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19280.23123272950568008No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18420.22091840650905742No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16920.20292830825913416No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15890.19057510746085354No Hit
GTCTTTGTCTCCGGGGGAAGGAGCCACCCTCTCCTGCAGGGCCAATCAGAGTGTTACCGGCTACTTAGCCTGGTA15510.18601761590420632No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15480.18565781393920786No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCGGCCTAGAGCCTGAAGATT15410.1848182760208781No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15250.18289933220755294No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15040.1803807184525637No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14800.17750230273257597No Hit
GACTTCACTCTCACCATCAGCGGCCTAGAGCCTGAAGATTTTGCAATTTATTATTGTCAACACCGTGGCGACTGG14130.16946672551427694No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13550.1625105541909733No Hit
GAGTGTTACCGGCTACTTAGCCTGGTACCAACACAAACCTGGCCAGGCTCCCAGGCTCCTCATCTTTGATACAT13300.15951220448265274No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13260.15903246852932146No Hit
GTTCAGGGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCGGCC13140.1575932606693276No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13110.15723345870432914No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12380.14847827755603316No Hit
GTCCCAGACCCACTGCCCCTGAACCTGCCCGGGATGCCAGTGGCCCTGTT12380.14847827755603316No Hit
GTCTCCAAGCACCCTGTCTTTGTCTCCGGGGGAAGGAGCCACCCTCTCCTGCAGGGCCAATCAGAGTGTTACCGG11930.14308124808105618No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCCCTGAACCTGCCCGGGATGCCA11830.14188190819772797No Hit
CTCCTGCAGGGCCAATCAGAGTGTTACCGGCTACTTAGCCTGGTACCAACACAAACCTGGCCAGGCTCCCAGGCT11750.1409224362910654No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11560.13864369051274178No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10630.12748982959778937No Hit
ACACAAACCTGGCCAGGCTCCCAGGCTCCTCATCTTTGATACATCTAACAGGGCCACTGGCATCCCGGGCAGGTT10600.1271300276327909No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC10270.1231722060178078No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9940.1192143844028247No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT9830.11789511053116365No Hit
GCCTACTACTGTGCGAGAGGCAGAGGGCGTTACTACGGTTTCGACGTCTGGGGCCAGGGGACCACGGTCATCGT9810.11765524255449801No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9670.1159761667178385No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT9630.11549643076450722No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9620.11537649677617438No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA9500.11393728891618055No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC9450.11333761897451641No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9230.11069907123119435No Hit
GGTTTGTGTTGGTACCAGGCTAAGTAGCCGGTAACACTCTGATTGGCCCT9070.10878012741786922No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9060.1086601934295364No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9050.10854025944120357No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8990.10782065551120663No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT8840.1060216456862143No Hit
GTCGTGTCCCTTGGCCGAAGGTGGGCCAGTCGCCACGGTGTTGACAATAA8640.10362296591955789No Hit
GTACCAACACAAACCTGGCCAGGCTCCCAGGCTCCTCATCTTTGATACATCTAACAGGGCCACTGGCATCCCGGG8590.10302329597789375No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8570.10278342800122812No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC8510.1020638240712312No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC8420.1009844181762358No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAA150.00247701968.128846
GGTATCA9500.041.26311
GTGGTAT2800.034.086911
TGGTATC2800.034.0848662
TATCAAC32150.034.0318572
TCAACGC32250.034.0197684
AACGCAG32600.033.9599276
ATCAACG32150.033.919733
CAACGCA32700.033.6477285
GACAGGT1950.033.2128831
ACGCAGA34500.032.0896727
CGCAGAG34750.031.46678
GCAGAGT35450.030.9414469
AGAGTAC35500.030.70595411
CGGGAAT803.0349784E-629.82246617
AGCGTTC703.947276E-529.46315837
GTATCAA39550.028.2692951
CAGAGTA39350.027.70168910
AATGGTA1357.0083677E-427.51000270
CGGGTAT500.008206665527.26625417
Produced by FastQC (version 0.11.5)