FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689215

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689215
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1424159
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC42880.30108997661075765No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA35220.24730384739344416No Hit
CCCAAAGGGGGCTGGTGATTGTATATGAGGTGCAATAATAATCAGCCTCG32150.22574726557919444No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG31750.22293859042424335No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT29310.20580567197904165No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG28620.200960707336751No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA28090.19723921275644082No Hit
GTAATACACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGAAGATATA27050.18993665735356796No Hit
GAATATCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTATTGCACCT24320.17076744942102673No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT24260.17034614814778407No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC23600.16571183414211477No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23210.16297337586603744No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT21820.15321322970258236No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC21520.15110672333636904No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCATCAGTGACATT21120.14829804818141795No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG21030.14766609627155394No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT20970.14724479499831128No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC20920.1468937106039424No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAAGGGGGCTG20250.14218917971939932No Hit
GTTATATATAGCGAAGACAGTATATATTACGCAGACTACGCAGACTCTGT20050.14078484214192374No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT19890.13966137207994334No Hit
CTCCTGCACTGGAACCATCAGTGACATTGGTGGTTATTCCTATGTCTCCTGGTACCAGCAGCACCCAGGCAAGGC19490.13685269692499222No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG19400.1362207450151282No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19200.1348164074376527No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG19110.13418445552778868No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA19090.13404402177004113No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT18870.13249925043481803No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC18660.1310246959784687No Hit
GTCGATCACCATCTCCTGCACTGGAACCATCAGTGACATTGGTGGTTATTCCTATGTCTCCTGGTACCAGCAGCA18500.12990122591648826No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG17810.1250562612741976No Hit
GATTATTATTGCACCTCATATACAATCACCAGCCCCCTTTGGGTGTTCGG17730.1244945262432074No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG17720.12442430936433363No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC17540.12316040554460563No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC17200.12077303166289718No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA17030.11957934472204296No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA17000.11936869408542164No Hit
CCTCATATACAATCACCAGCCCCCTTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG16890.11859630841781009No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC16730.11747283835582965No Hit
ATATATAACTGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCC16590.11648980205159677No Hit
GTGTATTACTGTGCGAGGTGGGGCCGGGGCCATTGTGGTGGTGAGTGCAGATACTACTACAACGGTCTGGACGT16590.11648980205159677No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16390.11508546447412123No Hit
CAGTAATACACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGAAGATA16150.11340025938115057No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA15970.11213635556142257No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT15720.11038093358957814No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT15580.10939789728534526No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC15480.10869572849660748No Hit
CTCCTGTGCAGCCTCTGAGTTCACCGTCAGTAGCAACCACATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG15450.10848507785998612No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG15400.10813399346561725No Hit
GTATATGAGGTGCAATAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGA14800.10392098073319062No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG14550.10216555876134618No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14280.10026970303175417No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)