FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689216

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689216
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1424159
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG68230.47908976455578345No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC37770.2652091515062574No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG33200.2331200378609411No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA32840.2305922302214851No Hit
CCCAAAGGGGGCTGGTGATTGTATATGAGGTGCAATAATAATCAGCCTCG31020.2178127582664576No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG29320.20587588885791547No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT27750.19485183887473237No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG27560.19351771817613062No Hit
GTAATACACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGAAGATATA25550.17940412552250135No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA23870.16760768987170674No Hit
GAATATCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTATTGCACCT23630.16592248477873608No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT23620.16585226789986232No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG22550.1583390618603681No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC22300.1565836398885237No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21900.15377496473357258No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC21060.1478767469081753No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC20900.14675327684619485No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT20050.14078484214192374No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCATCAGTGACATT19870.13952093832219578No Hit
GTCGATCACCATCTCCTGCACTGGAACCATCAGTGACATTGGTGGTTATTCCTATGTCTCCTGGTACCAGCAGCA19680.138186817623594No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC19570.13741443195598244No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG18750.1316566478883327No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT18530.1301118765531096No Hit
GTTATATATAGCGAAGACAGTATATATTACGCAGACTACGCAGACTCTGT18330.12870753897563403No Hit
CTCCTGCACTGGAACCATCAGTGACATTGGTGGTTATTCCTATGTCTCCTGGTACCAGCAGCACCCAGGCAAGGC18200.12779471955027494No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA17930.12589886382068294No Hit
GATTATTATTGCACCTCATATACAATCACCAGCCCCCTTTGGGTGTTCGG17570.12337105618122696No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT17290.1214049835727612No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG17160.12049216414740209No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG17100.12007086287415941No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAAGGGGGCTG16980.11922826032767408No Hit
GTGTATTACTGTGCGAGGTGGGGCCGGGGCCATTGTGGTGGTGAGTGCAGATACTACTACAACGGTCTGGACGT16940.11894739281217899No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC16700.11726218771920832No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG16660.1169813202037132No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA16150.11340025938115057No Hit
CCTCATATACAATCACCAGCCCCCTTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG16020.11248743995579147No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC15790.11087245174169458No Hit
CTCCTGTGCAGCCTCTGAGTTCACCGTCAGTAGCAACCACATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG15470.1086255116177337No Hit
ATATATAACTGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCC15430.10834464410223858No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA15330.1076424753135008No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC15180.10658922213039415No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15030.10553596894728749No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14950.10497423391629726No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA14880.10448271576418082No Hit
CCCTTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC14350.10076122118387063No Hit
CAGTAATACACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGAAGATA14290.10033991991062795No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15900.039.9719731
GTGGTAT5150.039.5963481
GTATCAA39300.035.7164761
TGGTATC5550.035.5215452
ATCAACG45050.031.3119543
TATCAAC44850.031.1484362
GCTTGCC1101.2441888E-930.897151
AACGCAG45700.030.792226
TCAACGC45700.030.6434654
CAACGCA46200.030.3118275
CGCAGAG47900.029.165088
TAGTAAC605.9160654E-428.3253733
CTCTGGA11750.028.23449169
AGAGTAC49400.028.06815111
CAGAGTA49650.027.93172510
GCAGAGT49850.027.8196649
CCGGTTA500.00830658227.19999919
ACAGGCC3300.026.777531
ACAGGTA2450.026.357161
GTGGCCG10900.026.34242470
Produced by FastQC (version 0.11.5)