FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689217

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689217
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1075890
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATGCTGTAGACAGTAATAAGTTGCAAAATCCTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTC45980.4273671100205411No Hit
GCATAATACTTACCCTCGGACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT44110.40998615100056696No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26540.24667949325674557No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24240.22530184312522655No Hit
GATCTATGCTGCATCCGCTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA23270.21628605154802072No Hit
CCCTTAGACTCTCCTGTGCAGCCTCTGGATTCACTTTCAGTAACGCCTGG22920.21303293087583303No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA20680.19221295857383192No Hit
GTCCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG20520.19072581769511754No Hit
GGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC19720.1832901133015457No Hit
CTCCTGTGCAGCCTCTGGATTCACTTTCAGTAACGCCTGGATGAGCTGGGTCCGCCAGGCTCCAGGGACGGGG18360.17064941583247356No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT18190.16906932864883956No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG18130.16851165081932168No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17560.16321371143890173No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT17370.16144773164542844No Hit
GGGCTGGAGTGGGTTGGCCGTATTAAAAGCGAAACTGATGGTGGGACAAC16780.15596389965516919No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG16760.1557780070453299No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16440.15280372528790118No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT15680.14573980611400794No Hit
CTCCAGCCCCGTCCCTGGAGCCTGGCGGACCCAGCTCATCCAGGCGTTAC15630.1452750745894097No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14640.13607339040236455No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14580.13551571257284667No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14520.1349580347433288No Hit
GCCTTGGGATTCCCAAGTGTTTTCATTCAGTGATCAGGACTGAACACAGA14290.13282026973017688No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGGCATTAGAAATGATTTGGCCTGGTATCAGCAGAAG14180.13179786037606075No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13950.12966009536290884No Hit
GATCAGGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGG13610.1264999209956408No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT13580.12622108208088187No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13510.12557045794644434No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG13490.12538456533660505No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13340.12399037076281033No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT13330.12389742445789068No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG13180.12250322988409595No Hit
ATACTTACCCTCGGACCTTCGGCCAAGGGACACGACTGGAGATTAAACGA13100.12175965944473878No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC13020.12101608900538159No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC13010.12092314270046195No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT12790.1188783239922297No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12780.11878537768731004No Hit
GCATAGATCAGGCGCTTAGGGGCTTTACCTGGCTTCTGCTGATACCAGGC12760.11859948507747076No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12440.115625203320042No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12440.115625203320042No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12360.11488163288068483No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA12280.11413806244132765No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG11990.11144261959865785No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT11980.11134967329373821No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11970.11125672698881857No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11900.11060610285438104No Hit
GGGTAGTCTGTTGTCCCACCATCAGTTTCGCTTTTAATACGGCCAACCCACTCCAGCCCCGTCCCTGGAGCCTGG11830.10995547871994349No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC11790.10958369350026488No Hit
GAGTTTGGGCTGAGCTGGGTTTTCCTTGCTGCTATTTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGT11710.1088401230609077No Hit
GGGTTCCCTGGCCCCAGTAGTCAAGCCACTCCAAAAATCGTAAGCCCCGCGACTGATCTGTGGTACAATAATACA11590.10772476740187195No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT11470.10660941174283615No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC11410.10605173391331826No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC11290.10493637825428251No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT11210.10419280781492532No Hit
ACTTATTACTGTCTACAGCATAATACTTACCCTCGGACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAA10990.10214798910669305No Hit
GTATCAACGCAGAGTACGGGAGCTCTGGGAGAGGAGCCCCAGCCTTGGGATTCCCAAGTGTTTTCATTCAGTGAT10970.10196209649685377No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)