FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689218

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689218
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1075890
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATGCTGTAGACAGTAATAAGTTGCAAAATCCTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTC48520.4509754714701317No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG43380.4032010707414327No Hit
GCATAATACTTACCCTCGGACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT41380.3846118097575031No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT28610.2659193783751127No Hit
GATCTATGCTGCATCCGCTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA21470.1995557166624841No Hit
CCCTTAGACTCTCCTGTGCAGCCTCTGGATTCACTTTCAGTAACGCCTGG20960.19481545511158205No Hit
CTCCTGTGCAGCCTCTGGATTCACTTTCAGTAACGCCTGGATGAGCTGGGTCCGCCAGGCTCCAGGGACGGGG19250.17892163697032223No Hit
GGGCTGGAGTGGGTTGGCCGTATTAAAAGCGAAACTGATGGTGGGACAAC18810.17483199955385773No Hit
GTCCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG18470.17167182518658972No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17830.16572326167173224No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17300.1607971075109909No Hit
GGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC16710.15531327552073165No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16380.1522460474583833No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG16330.15178131593378505No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT15990.14862114156651704No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15940.1481564100419188No Hit
CTCCAGCCCCGTCCCTGGAGCCTGGCGGACCCAGCTCATCCAGGCGTTAC15920.14797051743207948No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15780.1466692691632044No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15130.1406277593434273No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13990.13003188058258744No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13870.12891652492355166No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA13850.12873063231371237No Hit
GCCTTGGGATTCCCAAGTGTTTTCATTCAGTGATCAGGACTGAACACAGA13350.12408331706772997No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13160.12231733727425666No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC12600.11711234419875638No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC12510.11627582745447954No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12360.11488163288068483No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGGCATTAGAAATGATTTGGCCTGGTATCAGCAGAAG12320.11450984766100623No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG12280.11413806244132765No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12170.11311565308721153No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12030.11181440481833643No Hit
GATCAGGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGG11980.11134967329373821No Hit
GCATAGATCAGGCGCTTAGGGGCTTTACCTGGCTTCTGCTGATACCAGGC11950.11107083437897926No Hit
GGGTTCCCTGGCCCCAGTAGTCAAGCCACTCCAAAAATCGTAAGCCCCGCGACTGATCTGTGGTACAATAATACA11820.10986253241502383No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT11810.10976958611010419No Hit
GTATCAACGCAGAGTACGGGAGCTCTGGGAGAGGAGCCCCAGCCTTGGGATTCCCAAGTGTTTTCATTCAGTGAT11770.1093978008904256No Hit
GGGTAGTCTGTTGTCCCACCATCAGTTTCGCTTTTAATACGGCCAACCCACTCCAGCCCCGTCCCTGGAGCCTGG11750.1092119082805863No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT11650.10828244523138982No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT11610.10791066001171123No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11380.10577289499855934No Hit
ATACTTACCCTCGGACCTTCGGCCAAGGGACACGACTGGAGATTAAACGA11370.10567994869363968No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11370.10567994869363968No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG11260.10465753933952356No Hit
ACTTATTACTGTCTACAGCATAATACTTACCCTCGGACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAA11060.1027986132411306No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG10910.10140441866733588No Hit
GAGTTTGGGCTGAGCTGGGTTTTCCTTGCTGCTATTTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGT10820.10056790192305905No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10820.10056790192305905No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)