FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689219

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689219
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1302887
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATACTACCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT82970.6368165466383501No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT53830.41315939141306957No Hit
ATACTACCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA48740.37409230424434353No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC48070.3689498782319572No Hit
GTATAATATTGCTGACAGTGATAAACTGCCACATCTTCAGGCTGCAGGCTGTTGATGGTGAGAGTGAAATGTGTC43870.3367137748707294No Hit
GTAATAGACGGCTGTGTCTTCGATTTCCAGGCTGTTCATTTGCAGATATA36100.277076983652458No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC35050.26901795781215104No Hit
GGGTAGTATAATATTGCTGACAGTGATAAACTGCCACATCTTCAGGCTGC33270.2553559901971545No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG31080.2385471648730857No Hit
CTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGGACCCGGGATCCCT30010.2303346337786777No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29530.22665050768025163No Hit
ATTTTATACAGTTTCAACAATAAAAATTACTTAGCTTGGTACCAACAGAA28780.22089406065146094No Hit
ACTTAGGAGGCTGTCCTGGTTTCTGTTGGTACCAAGCTAAGTAATTTTTATTGTTGAAACTGTATAAAATACT28420.21813096607764143No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG28100.2156748820120241No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG27780.2132187979464067No Hit
CCCTTAGACTCTCCTGTGCAGCCTCTGGTTTCGCTTTCGGTAACGCCTGG27760.21306529269230565No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27530.21129998227014316No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25780.1978682725362982No Hit
CATTTCACTCTCACCATCAACAGCCTGCAGCCTGAAGATGTGGCAGTTTATCACTGTCAGCAATATTATACTACC25710.19733100414694443No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25710.19733100414694443No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25160.1931096096591646No Hit
GTCTATTACTGTGCCGCAGTCAAGGCATACTATGATAACGATGGTTATTTCTCAAACTGGTTCGACCCCTGGGGC23740.1822107366179876No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22810.17507274230228717No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22560.17315392662602358No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22400.17192588459321492No Hit
GTTTATCACTGTCAGCAATATTATACTACCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA21520.16517165341276718No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20350.15619159604785374No Hit
CTCCTGTGCAGCCTCTGGTTTCGCTTTCGGTAACGCCTGGATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGC19680.1510491700354674No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA19180.14721153868294026No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18930.14529272300667673No Hit
GTATAAAATACTCTGACTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA18510.14206911267055392No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT18290.14038055487544202No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18220.13984328648608818No Hit
AACCAGGACAGCCTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGGA17990.13807797606392572No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17840.1369266866581676No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG17840.1369266866581676No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGTCAGAGTATTTTATA17500.13431709733844915No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17420.1337030763220448No Hit
GGCCTGGAGTGGGTCGGCCGTATTAAGGGCAAAACTGATGGTGAGACAAT17050.13086322912117476No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16970.13024920810477042No Hit
GGACTGAACACAGAGGACTCCCCATGGAGTTTGGGCTGAGCTGGATTTTC16750.12856065030965846No Hit
ATTCAGTGGCAGCGGGTCTGGGACACATTTCACTCTCACCATCAACAGCC16700.12817688717440576No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16560.12710235039569817No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16480.12648832937929383No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGTCAGAGTATTTTATACAGTTTCAA16430.12610456624404112No Hit
CTCCAGGCCCTTCCCTGGAGCCTGGCGGACCCAGCTCATCCAGGCGTTAC16040.12311121378906996No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC15890.12195992438331182No Hit
CACCATCAACAGCCTGCAGCCTGAAGATGTGGCAGTTTATCACTGTCAGCAATATTATACTACCCCGCTCACTTT15820.12142265599395803No Hit
GCCTGAAGATGTGGCAGTTTATCACTGTCAGCAATATTATACTACCCCGC15770.12103889285870531No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT15370.11796878777668363No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG15290.1173547667602793No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG15010.1152056932028641No Hit
GTACCAACAGAAACCAGGACAGCCTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGGACCCGGGATCCCTGA15010.1152056932028641No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14880.11420790905120705No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14560.11175182498558969No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14500.11129130922328645No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14230.10921898829292179No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14160.108681719903568No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14160.108681719903568No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG14060.10791419363306257No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14030.10768393575191093No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14020.10760718312486041No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13660.10484408855104088No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13270.10185073609606972No Hit
GATCAGGACTGAACACAGAGGACTCCCCATGGAGTTTGGGCTGAGCTGGA13150.1009297045714632No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA13060.10023893092800833No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG13040.10008542567390726No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10900.045.8137971
TCTTGAG11950.039.52013870
GTGGTAT2900.038.921171
TCGTATG2400.038.83678440
CGAGACG2750.038.55513823
ATCTCGT2600.038.38435437
CACGAGA2950.037.0920821
GATTCAC1800.036.1036451
GTATCAA25500.035.947161
CGTATGC2500.035.9261241
CTTATAC3150.035.8321881
ATTAGAG1450.035.3638733
TGGTATC3050.034.7454832
CCGTCTT2900.034.72344247
ATGCCGT2800.034.6383844
ACGAGAC3200.034.1942622
TCTCCGA3300.033.15043311
TAACGGC1154.9112714E-1132.891936
CATCTCG3250.032.79989236
CGAGCCC3450.032.7012815
Produced by FastQC (version 0.11.5)